Producing method of functional quantum point used for specific recognizating tumor cell
A technology of tumor cells and quantum dots, applied in biological testing, material inspection products, etc., can solve problems such as non-specific staining, and achieve the effects of safe raw materials, safe and simple operation, and convenient preparation
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Embodiment 1
[0026] 1. Preparation of quantum dots
[0027] (1) Prepare the solution of AOT and n-heptane
[0028] Prepare 30 mL of 0.1 M AOT solution in n-heptane.
[0029] (2) Configuration of reverse micellar system
[0030] Add CdCl to the above solution 2 aqueous solution, so that the concentration of the aqueous phase in the system is 0.1M. Add CdCl 2 After the aqueous solution was dispersed by ultrasonic waves for 30 minutes, until the system was clear and transparent, a reverse-phase micromicelle system was obtained.
[0031] (3) Reaction to generate CdS quantum dots
[0032] At room temperature, nitrogen gas was passed into the above-mentioned reversed-phase micromicelle system for 5 minutes to remove the oxygen in the system, and then under the condition of magnetic stirring, sufficient H was introduced into the above-mentioned micromicelle system. 2 S gas. When the color of the system no longer changes, it means that the reaction is complete and the quantum dots have been...
Embodiment 2
[0043] The greatest creativity of the method of the present invention lies in the step of functional modification of the surface of quantum dots (that is, linking of quantum dots and folic acid). This step is:
[0044] After the surface of the prepared quantum dots was treated by a small molecular compound containing mercapto groups, wash with acetone 3-5 times, wash, centrifuge, and dry alternately; -30 μL 0.05M NHS DMSO solution and the same volume of 0.05M EDC DMSO solution, stirred for 30-60 minutes, added 20-30 μL 0.05M folic acid DMSO solution, stirred, and reacted for 12-24 hours. Finally, the reactant was dialyzed with deionized water for 5-8 hours to remove unreacted small molecules.
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