Producing method of functional quantum point used for specific recognizating tumor cell

A technology of tumor cells and quantum dots, applied in biological testing, material inspection products, etc., can solve problems such as non-specific staining, and achieve the effects of safe raw materials, safe and simple operation, and convenient preparation

Inactive Publication Date: 2007-06-06
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Quantum dots used for biological research must have specific recognition functions for different types of cells. The existing quantum dots c

Method used

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  • Producing method of functional quantum point used for specific recognizating tumor cell
  • Producing method of functional quantum point used for specific recognizating tumor cell
  • Producing method of functional quantum point used for specific recognizating tumor cell

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1:

[0026] 1. Preparation of quantum dots

[0027] (1) Prepare a solution of AOT and n-heptane

[0028] Prepare 30 mL of 0.1M AOT in n-heptane solution.

[0029] (2) Configuration of reverse micelle system

[0030] Add CdCl to the above solution 2 The concentration of the aqueous phase in the system is 0.1M. Join CdCl 2 After the aqueous solution is dissolved, use ultrasonic dispersion for 30 minutes until the system is clear and transparent, thus obtaining a reversed-phase micromicelle system.

[0031] (3) Reaction to generate CdS quantum dots

[0032] At room temperature, bubbling nitrogen into the reversed-phase micromicelle system for 5 minutes to remove the oxygen in the system, and then under the condition of magnetic stirring, add sufficient H into the micromicelle system. 2 S gas. When the color of the system no longer changes, the reaction is complete and the quantum dots have been successfully prepared.

[0033] 2. Surface modification of thioglycolic aci...

Example Embodiment

[0042] Example 2:

[0043] The greatest creativity of the method of the present invention lies in the step of functional modification of the surface of the quantum dots (that is, the connection of the quantum dots and folic acid). This step is:

[0044] After surface of the prepared quantum dots with sulfhydryl-containing small molecule compounds, wash 3-5 times with acetone, wash, centrifuge, and dry alternately; then, disperse 5 mg of quantum dot powder in 5-10 mL PBS, and add 20 -30 μL of 0.05M NHS in DMSO solution and the same volume of 0.05M EDC in DMSO solution, stirring for 30-60 minutes, adding 20-30 μL of 0.05M folic acid in DMSO solution, stirring, and reacting for 12-24 hours. Finally, the reactant was dialyzed with deionized water for 5-8 hours to remove unreacted small molecules.

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Abstract

A preparing method of the functional quanta point to identify the tumor cell specially belongs to the nanometer material and biological field. The method includes the below process: (1) preparing the CdS quanta point; (2) modifying the surface by small molecular compound with the mercapto; (3) the functional modification to the quanta point surface: add the DMSO solution with the NHS and the EDC to form a high activity midbody which can react with the amido in the folic acid. The mercapto can improve the water solution. So the fluorescent quanta point can identify the tumor cell expressing the high level folic acid acceptor, so it can be used as the fluorescent probe in cell biological study.

Description

Technical field [0001] A method for preparing functionalized quantum dots for specific recognition of tumor cells belongs to the field of nanometer materials and biotechnology of the present invention. Background technique [0002] Tumor is the number one disease that harms all human beings. Early diagnosis, early treatment and targeted therapy of tumors are the content that people have been paying attention to and researching. Traditional fluorescent dyes used in cell biology research have many limitations that cannot be overcome, such as strict requirements on excitation wavelength, low fluorescence efficiency, easy overlapping of emission wavelength and excitation wavelength, poor photostability, etc., which bring great challenges to biological research. A lot of inconvenience. [0003] With the development of technology, the research and development focus of nanotechnology has gradually shifted from the synthesis of a single nanomaterial to the function, application, a...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 张培根余德才贺志鹏张海黔
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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