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Culture medium for liquid deep fermentation for producing truffle polysaccharide

A technology of liquid submerged fermentation and culture medium, which is applied in the field of medium for the production of truffle polysaccharide by liquid submerged fermentation, can solve the problems of high price of raw materials, great difficulty in manual discovery, large labor and material resources consumption, etc., and achieves reduction of production cost, Avoid limited raw materials, avoid expensive effects

Inactive Publication Date: 2007-07-11
HUBEI UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0011] 2. In order to make up for the lack of natural yield of truffles, many scholars have conducted research on semi-artificial simulated cultivation, but it usually takes 7-9 years from inoculation to harvest, which is a long cycle and requires a lot of manpower and material resources
[0012] 3. Since truffles grow underground, it is difficult to find artificially, and they are easily damaged during the collection process. At the same time, truffles growing in nature are greatly affected by environmental changes, and the quality is difficult to guarantee
Therefore, if the fruiting bodies of truffles are directly used as raw materials for the production of truffle polysaccharides, problems such as expensive raw materials and limited output cannot be avoided.
[0014] The current literature reports only focus on the pure culture of the physiological characteristics of Chinese truffles and the preliminary research on the mycelium growth of truffles in the semi-artificial simulated cultivation process of black spore truffles, which involves submerged fermentation The research on the pure culture of truffles producing truffle polysaccharide has not yet seen the relevant reports, and the relevant research on the medium formula belongs to the first report in the present invention

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] The strain used: Chinese truffle (Tuber sinense Taoet Liu).

[0026]A, slant strain culture: the formula of the slant strain culture medium adopted in this embodiment is: maltose 50 grams / liter, magnesium sulfate 5 grams / liter, potassium dihydrogen phosphate 5 grams / liter, agar 15 grams / liter, potato Extract 1.0 L, pH 5 or 6 or 7 or 8. Sterilization conditions are: 121°C, 20 minutes. The culture temperature is 15 or 17 or 19 or 24 or 27 or 29 or 34 or 36 or 40° C., and the culture time is 2 or 5 or 7 or 9 or 14 or 16 or 17 or 20 days.

[0027] B, liquid seed cultivation: in the present embodiment, the formula of the first-level liquid seed and the second-level liquid seed culture medium is: maltose 70 grams / liter, peptone 25 grams / liter, magnesium sulfate 5 grams / liter, potassium dihydrogen phosphate 6 g / l, pH 5 or 6 or 7 or 8. The first-level liquid seed culture can be carried out by transferring the slant bacteria into the shake flask equipped with the first-level ...

Embodiment 2-4

[0031] The carbon sources in the liquid submerged fermentation medium were 80 g / L lactose, 80 g / L fructose, and 80 g / L galactose respectively, and other conditions were the same as in Example 1. Analyzing and testing the results, the yields of exopolysaccharides from truffles were 1.83 g / L, 1.13 g / L, and 1.17 g / L, respectively.

Embodiment 5-12

[0033] The carbon source in the liquid submerged fermentation medium is lactose, and the concentrations are 30 g / L, 50 g / L, 60 g / L, 70 g / L, 90 g / L, 110 g / L, 130 g / L, 150 g / L g / L, other conditions are the same as in Example 1. Analysis and test results show that the yields of truffle exopolysaccharides are 0.88 g / L, 1.15 g / L, 1.35 g / L, 2.18 g / L, 1.60 g / L, 1.35 g / L, 1.19 g / L, 0.91 g / Lift.

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Abstract

The invention discloses a culture medium of liquid deep-layer ferment manufacturing block granulose, which is characterized by the following: allocating the culture medium through lactin, peptone, magnesium sulfate and potassium dihydrogen phosphate according to certain proportion; making inclined bacteria; culturing liquid seed; fermenting liquid; considering different carbon sources, nitrogen sources and original pH values to produce the granulose.

Description

technical field [0001] The invention belongs to the field of bioengineering and technology, and in particular relates to a culture medium for producing truffle polysaccharide by submerged liquid fermentation, and also relates to a method for submerged fermentation of truffle polysaccharide. The medium is suitable for submerged fermentation of truffle And industrialized large-scale fermentation to produce truffle polysaccharide. technical background [0002] Truffle is one of the most precious medicinal and edible fungi in the world. Its ascomycetes are spherical, hemispherical or block-shaped, generally 1.5-12 cm in diameter, brown, reddish-brown to dark brown when mature, and often concave at the base , with small warts of different sizes on the surface. The origin of truffles is narrow, mainly distributed in the northern temperate and frigid regions of the European continent. Its output has been in short supply in the market, and its value is outstanding. [0003] Truffl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04
Inventor 汤亚杰李冬生孔国平
Owner HUBEI UNIV OF TECH
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