Sphingol single-cell bacteria and method for producing microorganism polysaccharide- 'sanzan' gum using the strain

Abstract

The invention discloses a making method of sphingol single-cell bacterium and microbe polysaccharide, which comprises the following steps: inoculating sphingol single-cell bacterium CGMCCNo. 1650 in the culture liquid with carbon source; adding necessary nitrogen and nourishing material; adjusting pH value of culture liquid; aerating; stirring; fermenting; adjusting isoelectric point through neutral salt for fermented liquid; sedimenting; extracting; dehydrating; obtaining the product.

Description

Technical field [0001] The invention relates to a preparation method of Sphingomonas and microbial polysaccharides. Background technique [0002] At present, xanthan gum is the representative product of microbial polysaccharides. In the preparation process, Xanthomonas vascularis, Xanthomonas begonia, Xanthomonas wild rape and other strains in the genus Xanthomonas are used as fermentation strains In the extraction process, organic solvent-ethanol is used as the extractant or acid extraction is used as the main extraction method. The finished microbial polysaccharide-xanthan gum product produced by the fermentation method of the above-mentioned strains is yellow or light yellow. , And the product's aqueous solution viscosity (800cp), shear performance value (6.3) and temperature resistance are all poor. Due to its low aqueous solution viscosity and shear performance value and other main technical indicators, the product is in the process of promotion and application There are gre...

AI Technical Summary

Problems solved by technology

[0002] At present, microbial polysaccharides represented by xanthan gum are prepared by using Xanthomonas vascularus, Xanthomonas begonia, Xanthomonas campestris and other bacteria in the genus Xanthomonas as fermentation strains. , obtained by using organic solvent-ethanol as the extraction agent or acid extraction as the main extraction method in the extraction process, the finished microbial polysaccharide made by the fermentation method of the above-mentioned strains - xanthan gum product color is yellow or light yellow , and the product's aqueous solution viscosity (800cp), shear performance value (6.3) and temperature resistance are poor. Due to the low main technical indicators such as its aqueous solution viscosity and shear performance value, the product is in the process of popularization and application. there are great difficulties

In the extraction process after fermentation, organic solvents and acids are used as extractants, which have problems such as easy volatility, low safety factor during use, and high cost, which bring great safety hazards to industrial production.