Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product

a technology of polyisoprenoid and transformed plant, which is applied in the direction of transferase, peptide sources, other domestic articles, etc., can solve the problems of insufficient understanding of the association between cpt and cpt, limited period during which natural rubber can be extracted, and difficult to greatly increase the production of natural rubber using hevea brasiliensis, etc., to achieve the effect of enhancing the activity of the cpt family protein, enhancing the activity of rubber

Active Publication Date: 2019-08-20
SUMITOMO RUBBER IND LTD +1
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach stabilizes and enhances rubber synthesis activity, leading to increased natural rubber production efficiency and the ability to produce environmentally friendly rubber products like pneumatic tires without continuous chemical treatment.

Problems solved by technology

Moreover, Hevea brasiliensis requires about seven years from planting to mature enough for rubber extraction, and the period during which natural rubber can be extracted is limited to 20 to 30 years.
Although more natural rubber is expected to be demanded mainly by developing countries in years to come, for the reason mentioned above it is difficult to greatly increase the production of natural rubber using Hevea brasiliensis.
However, the associations between these proteins and CPT are not completely understood.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product
  • Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product

Examples

Experimental program
Comparison scheme
Effect test

example 1

Extraction of Total RNA from Hevea Latex

[0246]Total RNA was extracted from the latex of Hevea brasiliensis by the hot phenol method. To 6 mL of the latex were added 6 mL of 100 mM sodium acetate buffer and 1 mL of a 10% SDS solution, and then 12 mL of water-saturated phenol pre-heated at 65° C. The mixture was incubated for 5 minutes at 65° C., agitated in a vortex, and centrifuged at 7000 rpm for 10 minutes at room temperature. After the centrifugation, the supernatant was transferred to a new tube, 12 mL of a phenol:chloroform (1:1) solution was added, and the mixture was agitated by shaking for 2 minutes. After the agitation, the resulting mixture was centrifuged again at 7000 rpm for 10 minutes at room temperature, the supernatant was transferred to a new tube, 12 mL of a chloroform:isoamyl alcohol (24:1) solution was added, and the mixture was agitated by shaking for 2 minutes. After the agitation, the resulting mixture was centrifuged again at 7000 rpm for 10 minutes at room t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
Login to View More

Abstract

Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.

Description

REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB[0001]This application includes an electronically submitted sequence listing in .txt format. The .txt file contains a sequence listing entitled “2016-11-01 5051-0413PUS1 ST25.txt” created on Nov. 1, 2016 and is 10,519 bytes in size. The sequences listing contained in this .txt file is part of the specification and is hereby incorporated by reference herein in its entirety.TECHNICAL FIELD[0002]The present invention relates to a method for producing a polyisoprenoid, a transformed plant, a method for producing a pneumatic tire, and a method for producing a rubber product.BACKGROUND ART[0003]Nowadays natural rubber (one example of polyisoprenoids) for use in industrial rubber products are obtained by cultivating rubber-producing plants, such as para rubber tree (Hevea brasiliensis) belonging to the family Euphorbiaceae, or Indian rubber tree (Ficus elastica) belonging to the family Moraceae, to biosynthesize natural rubber by the lacti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(United States)
IPC IPC(8): C12N15/82C12P5/00C12N9/10B29D30/08B29D30/06C07K14/415B29K607/00
CPCC12P5/007B29D30/0601B29D30/08C12N9/1085C12N15/8243C07K14/415C12Y205/0102B29K2607/00
Inventor INOUE, YUKINOYAMAGUCHI, HARUHIKOFUSHIHARA, KAZUHISATAKAHASHI, SEIJIYAMASHITA, SATOSHINAKAYAMA, TORU
Owner SUMITOMO RUBBER IND LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com