Improved immortalized human skin cell lines and novel serum-free medium useful for the production thereof

a technology of immortalized human skin and serum-free medium, which is applied in the direction of cell culture active agents, artificial cell constructs, genetically modified cells, etc., can solve the disadvantages of their use, achieve moderate effect on keratinocyte cell growth, improve both the attachment of keratinocytes, and enhance normal keratinocyte growth
US20020012993A1Inactive Publication Date: 2002-01-31NESTEC SA
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
NESTEC SA
Publication Date
2002-01-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.
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Description

[0001] The present invention relates to improved continuous (immortalized) cell lines derived from normal human skin tissues, in particular keratinocytes and melanocytes which retain the ability to express differentiation proteins characteristic of differentiated melanocytes or keratinocytes, even in high passages. The present invention also relates to novel serum free media which do not require the use of feeder cells.

[0002] The production of immortalized cell lines derived from human skin tissues has been previously described. In general such methods comprise transfection or transformation of human skin cells, e.g., keratinocytes and melanocytes, cultured in vitro with agents which provide for immortalization.

[0003] Immortalization refers to the production of cells which are able to be cultured for prolonged time periods in vitro, ideally indefinitely. These cells are also referred to as continuous cell lines. By contrast non-immortalized cells are only capable of growing for a fi...

Claims

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