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Novel polynucleotides and polypeptides in pathogenic mycobacteria and their use as diagnostics, vaccines and targets for chemotherapy

a technology of mycobacteria and polypeptides, which is applied in the field of new polynucleotides and polypeptides in pathogenic mycobacteria and their use as diagnostics, vaccines and targets for chemotherapy, can solve the problems of resistant organisms, strains are unable to repair the constructed deletion mutation, and the truncated gene products cannot carry out the normal function of the gen

Inactive Publication Date: 2004-12-23
ST GEORGES HOSPITAL MEDICAL SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"This patent describes a new polynucleotide sequence called GS, which is found in pathogenic mycobacteria such as Mptb and Mavs. The GS sequence is a mobile package of DNA that helps bring about the conversion of a non-disease-causing strain to a disease-causing strain. The patent also describes a method for detecting and characterizing the GS sequence using DNA-based differential analysis technology. The invention is useful for improving the diagnosis, prevention, and treatment of tuberculosis and other mycobacterial diseases."

Problems solved by technology

Drugs used to treat tuberculosis are increasingly encountering the problem of resistant organisms.
The term thus envisages deletion of the entire gene or substantial portions thereof, and also point mutations in the coding sequence which result in truncated gene products unable to carry out the normal function of the gene.
Once the recA mutation has been incorporated the strain will be unable to repair the constructed deletion mutations.

Method used

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  • Novel polynucleotides and polypeptides in pathogenic mycobacteria and their use as diagnostics, vaccines and targets for chemotherapy

Examples

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example 2

[0151] To obtain the full sequence of GS in Mavs and Mptb we generated a genomic library of Mavs using the restriction endonuclease EcORI and cloning into the vector pUC18. This achieved a representative library which was screened with .sup.32P-labelled identifier sequence yielding a positive clone containing a 17 kbp insert. We constructed a restriction map of this insert and identified GS as fragments unique to Mavs and Mptb and not occurring in laboratory strains of M.avium. These fragments were sub-cloned into pUC18 and pGEM4Z. We identified GS contained within an 8 kb region. The full nucleotide sequence was determined for GS on both DNA strands using primer walking and automated DNA sequencing. DNA sequence for GS in Mptb was obtained using overlapping PCR products generated using PwoDNA polymerase, a proofreading thermostable enzyme. The final DNA sequences were derived using the University of Wisconsin GCG gel assembly software package.

example 3

[0152] The DNA sequence of GS in Mavs and Mptb was found to be more than 99% homologous. The ORFs encoded in GS were identified using GeneRunner and DNAStar computer programmes. Eight ORFs were identified and designated GSA, GSB, GSC, GSD, GSE, GSF, GSG and GSH. Database comparisons were carried out against the GenEMBL Database release version 48.0 (9 / 96), using the BLAST and BLIXEM programmes. GSA and GSB encoded proteins of 13.5 kDa and 30.7 kDa respectively, both of unknown functions. GSC encoded a protein of 38.4 kDa with a 65% homology to the amino acid sequence of rfbD of V. cholerae, a 62% amino acid sequence homology to gmd of E. coli and a 58% homology to gca of Ps.aeruginosa which are all GDP-D-mannose dehydratases. Equivalent gene products in H.influenzae, S.dysenteriae, Y.encerocolitica, N.gonorrhoea, K.pneumoniae and rfbD in Salmonella enterica are all involved in `O`-antigen processing known to be linked to pathogenicity. GSD encoded a protein of 37.1 kDa which showed ...

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Abstract

The invention provides a nucleotide sequence representing a pathogenicity island found in species of pathogenic mycobacteria. The islands are shown as SEQ ID NOS: 3 and 4 and comprises several open reading frames encoding polypeptides. These polypeptides and their use in diagnosis and therapy form a further aspect of the invention.

Description

[0001] This invention relates to the novel polynucleocide sequence we have designated "GS" which we have identified in pathogenic mycobacteria. GS is a pathogenicity island within 8 kb of DNA comprising a core region of 5.75 kb and an adjacent transmissable element within 2.25 kb. GS is contained within Mycobacterium paratuberculosis, Mycobacterium avium subsp. silvaticum and some pathogenic isolates of M.avium. Functional portions of the core region of GS are also represented by regions with a high degree of homology that we have identified in cosmids containing genomic DNA from Mycobacterium tuberculosis.BACKGROUND TO THE INVENTION[0002] Mycobacterium tuberculosis (Mtb) is a major cause of global diseases of humans as well as animals. Although conventional methods of diagnosis including microscopy, culture and skin testing exist for the recognition of these diseases, improved methods particularly new immunodiagnostics and DNA-based detection systems are needed. Drugs used to treat...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/00C07K14/35C12N15/31
CPCA61K38/00C07K14/35
Inventor HERMON-TAYLOR, JOHNDORAN, TIMMILLAR, DOUGLASTIZARD, MARKLOUGHLIN, MARKSUMAR, NAZIRAFORD, JOHN
Owner ST GEORGES HOSPITAL MEDICAL SCHOOL
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