Method of using fish plasma components for tissue culture

a technology of plasma components and fish, which is applied in the field of tissue culture, can solve the problems of compromising quality, unable to achieve 100% inactivation without compromising quality, and affecting the quality of tissue culture, so as to overcome the cytotoxicity of fish whole serum, unique
US20050164389A1Inactive Publication Date: 2005-07-28SAWYER EVELYN S +2
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
SAWYER EVELYN S
Publication Date
2005-07-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

A tissue culture substrate for culturing mammalian stem cell tissue includes one or more specific fish plasma fractions, isolated from any plasma remainder, in a nutrient medium. The plasma fractions are extracted from plasma obtained from the blood of one or more fish that are progeny of domesticated broodstock reared under consistent and reproducible conditions.
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Description

CROSS-REFERENCE TO RELATED APPLICATION

[0001] This is a continuation-in-part of co-pending U.S. patent application Ser. No. 09 / 907,443, filed on Jul. 18, 2001.FIELD OF THE INVENTION

[0002] The present invention relates generally to the culture of tissue, including cells and organs, and more specifically to the culture of mammalian tissue using at least one component of plasma derived from fish. The method has significant advantages over the more commonly used technique of utilizing serum or plasma components derived from humans or cows, or the more recently developed technique of utilizing whole serum or plasma from fish. BACKGROUND OF THE INVENTION

[0003] Tissue culture, the production of living tissue in vitro, permits numerous applications that would be difficult or impossible in a living organism. These applications include in vitro applications such as diagnosing disease and assessing toxicity, and more recently, the production of therapeutics, including vaccines and recombinan...

Claims

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