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Treatment of wounds

a wound and wound technology, applied in the field of wound treatment, can solve the problems of untreatable wounds, little literature on the way in which these larvae are treated, and only partially effective substances

Inactive Publication Date: 2007-03-22
THE SEC OF STATE FOR DEFENCE IN HER BRITANNIC MAJESTYS GOVERNMENT OF THE UK OF GREAT BRITAIN & NORTHERN IRELAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The purified enzyme and peptides effectively promote wound healing by degrading extracellular matrix components, preventing fibrin cuff formation, stimulating angiogenesis, and inducing growth factors, thus enhancing wound regeneration without the drawbacks of live maggot treatments.

Problems solved by technology

Clinical trial data indicate that such substances are only partially effective in promoting the healing of wounds.
However, little has been reported in the literature about the way in which these larvae go about their task of cleaning wounds to an extent that conventionally untreatable wounds heal.
Although efficacious, live larvae are unpleasant to many patients and the use of live larvae on wounds and the introduction of their crude secretions into wounds, which inevitably occurs when the larvae are used, are unacceptable to many patients and to many medical practitioners.
The use of live organisms also increases the risk of infection or allergic reactions in the patient.

Method used

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  • Treatment of wounds
  • Treatment of wounds
  • Treatment of wounds

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Experimental program
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Embodiment Construction

[0024] The protein of the present invention exists, in nature, in the excretory / secretory (ES) secretions of the larvae of Lucilia sericata.

[0025] The larval ES secretions demonstrate a classical pH optimum of 8.0-8.5 when hydrolysing the fluorescent protein substrate fluorescein isothiocyanate-casein (FITC-casein). By pre-incubating the larval ES secretions, prior to monitoring the hydrolysis of FITC-casein, with the irreversible low molecular weight inhibitors 4-(amidinophenyl) methane sulphonyl fluoride (APMSF; an inhibitor for all trypsin-like serine proteases but not chymotrypsin-like serine proteinases) or with phenyl methanesulphonyl fluoride (PMSF; an inhibitor for all serine proteinases) it is shown that larval ES secretions have two types of serine proteinase activity; a trypsin-like activity and a chymotrypsin-like activity. The dual activity is confirmed by monitoring the hydrolysis of the fluorescent peptide substrates Tosyl-Gly-Pro-Arg-AMC (selective for trypsin-like ...

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Abstract

An isolated protein, for use in treatment of wounds, is characterized in that it is secreted by the organism Lucilia sericata and it exhibits proteolytic activity against FITC-casein at a pH of 8.0 to 8.5. The protein exhibits proteolytic activity against Tosyl-Gly-Pro-Arg-AMC but not against Suc-Ala-Ala-Phe-AMC, and its proteolytic activity against FITC-casein and Tosyl-Gly-Pro-Arg-AMC is inhibited by the serine proteinase inhibitors PMSF and AMPSF. The protein is also bound by immobilized aminobenzamidine.

Description

[0001] This application is a division of application Ser. No. 10 / 111,252, filed on Aug. 23, 2002, issuing, which is a U.S. national phase application, pursuant to 35 U.S.C. §371, of PCT international application Ser. No. PCT / GB00 / 04034, filed on Oct. 20, 2000, designating the United States and published in English on May 3, 2001 as publication WO 01 / 31033 A2, which claims priority to application Ser. No. GB 9925005.2, filed on Oct. 22, 1999. The entire contents of the aforementioned patent applications are incorporated herein by this reference.TECHNICAL FIELD [0002] The present invention relates to the treatment of wounds. More particularly, it relates to substances which promote the healing of wounds, to compositions and to dressings which incorporate such substances and to a method of treating wounds using such substances. BACKGROUND TO THE INVENTION [0003] Efficient wound healing is a complex physiological process which involves many mechanisms including cell migration, growth fa...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/08C07K7/06A61K38/48C12N9/48A61K38/00A61L15/32C07K14/435C12N9/64C12N15/57
CPCA61K38/00C07K14/43577A61L15/32
Inventor PRITCHARD, DAVID IDRIS
Owner THE SEC OF STATE FOR DEFENCE IN HER BRITANNIC MAJESTYS GOVERNMENT OF THE UK OF GREAT BRITAIN & NORTHERN IRELAND