Methods of identifying small molecules for renewal, survival and migration of cardiac progenitors

a small molecule and cardiac progenitor technology, applied in the field of high-throughput screening assay, can solve the problem that the spatial requirement for wnt signaling has not been addressed

Inactive Publication Date: 2008-05-08
RGT UNIV OF CALIFORNIA
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  • Abstract
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Benefits of technology

[0024]FIGS. 9A-9Z are pictorial diagrams showing analysis of potential downstream effector targets in Isl1-Cre; β-catenin mutants and control littermates. Results from whole mount RNA in situ hybridization assays.
[0025]FIGS. 10A-10F are graphical diagrams showing analysis of potential downstream effector targets in Isl1-Cre; β-catenin mutants and control littermates. Results from Real-Time qPCR analyses. Within FIGS. 10A-10F, the following abbreviations mean: Con, Isl1-Cre / +; β-catenin+ / f; Mu, Isl1-Cre / +; β-catenin- / f
[0026]FIGS. 11A-11 P are pictorial diagrams showing that β-catenin is efficiently ablated in Isl1 expressing lineages or their descendents, including foregut endoderm, OFT myocardium and RV myocardium in Isl1-Cre; β-catenin mutants. Within FIGS. 11A-11P, the following abbreviations mean: FE, Foregut endoderm; OFT, outflow tract; RV, right ventricle; LV, left ventricle.

Problems solved by technology

In these cell culture systems, however, the spatial requirement for Wnt signaling has not been addressed.

Method used

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  • Methods of identifying small molecules for renewal, survival and migration of cardiac progenitors
  • Methods of identifying small molecules for renewal, survival and migration of cardiac progenitors
  • Methods of identifying small molecules for renewal, survival and migration of cardiac progenitors

Examples

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example 1

Lineage Diversification of ISL1+ Cardiac Progenitor Cells

[0091]Isl1 plays a pivotal role in development of cardiac progenitors of the second heart field, marks postnatal progenitors in postnatal heart, and marks cardiovascular progenitor cells in the early embryo which are pluripotent in vitro. Therefore, understanding factors which regulate Isl1 expression is critical to understanding factors which drive cardiac progenitor proliferation, survival and migration, both in the context of normal development, and for potential application to cell therapies utilizing cardiac progenitors.

[0092]The purification, renewal and differentiation of native cardiac progenitors would form a mechanistic underpinning for unraveling steps for both cardiac lineage formation and regeneration, and their links to forms of congenital and adult cardiac diseases. Until now there has been little evidence for native cardiac precursor cells in the post-natal heart.

[0093]Recently, taking advantage of a developmen...

example 2

WNT Signaling Function In Self-Renewal of Cardiac Progenitor Cells

[0103]The canonical Wnt cascade has emerged as a critical regulator of stem cells. Stem cells are cells that have the unique ability to self-renew as well as to generate more differentiated progeny. The most primitive stem cell is the embryonic stem cell, which is derived from the inner cell mass of the blastocyst. This cell is pluripotent and can thus generate all tissues of the body. Adult stem cells are normally involved in homeostatic self-renewal processes but can also be rapidly recruited to repair tissues upon injury, their self renewal capacity is limited. The importance of the Wnt cascade was established over the last years for stem cell maintenance and growth in the intestinal, epidermal and hematopoietic system (Reya T. & Clevers H (2005) Wnt signaling in stem cells and cancer. Nature 434, 843-850).

[0104]Wnt genes, of which the human genome harbours almost 20, occur throughout the animal kingdom (Veernan M ...

example 3

β-Catenin Regulates Islet1 Expression In Cardiovascular Progenitors

[0122]Floxed β-catenin mice were obtained from the Max-Planck-Institute of Immunobiology. Isl1-Cre mice were created by a Cre knock-in into the endogenous Isl1 locus, replacing the endogenous Isl1 ATG. Homozygous floxed β-catenin mice were crossed with Protamine-Cre mice to generate β-catenin + / − mice, which were then crossed with Isl1-Cre mice to produce doubly heterozygous Isl1-Cre+ / −; β-catenin + / − mice. These mice were then crossed to β-catenin floxed / floxed homozygous mice to obtain Isl1 -Cre+ / −; β-cat - / f mutants for analysis.

[0123]Whole-mount RNA in situ hybridization and histological analyses. Whole-mount RNA in situ hybridization was carried out as previously described. References for specific RNA in situ probes are as follow: Isl1 (EST, GenBank Accession No.: AA198791), Tbx2, Tbx3 and Pitx2 were from the Institute of Neurosciences, University of Padua, Padua, Italy; Wnt11 was from The Department of Molecula...

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Abstract

The present invention relates to a small molecule high-throughput screening assay consisting of detectably labeled cardiac progenitor cells. The invention also describes a method of identifying small molecules from the high-throughput assay affecting cardiogenesis and/or modulating cardiac progenitor cell development. Also described are methods of stimulating maturation of cardiac progenitor cells using a GSK-3β inhibitor.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part under 35 U.S.C. §120 of co-pending U.S. application Ser. No. 10 / 544,053, filed Apr. 13, 2006, and claims the benefit of priority under 35 U.S.C. §119(e) of U.S. Ser. No. 60 / 797,338, filed May 2, 2006, the entire content of which is incorporated herein by reference.GRANT INFORMATION[0002]This invention was made in part with government support under Grant Nos. R01 HL74066, R01 HL70867 and AHA 0525141Y awarded by the National Institutes of Health. The United States government may have certain rights in this invention.FIELD OF THE INVENTION [0003]The present invention relates generally to a high-throughput screening assay and more specifically to a method of identifying small molecules affecting cardiac progenitor cells.BACKGROUND INFORMATION[0004]The heart is composed of diverse muscle and non-muscle cell lineages: atrial / ventricular cardiac myocytes, conduction system cells of the working myocardiu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/53
CPCA01K67/0275C12Q2600/136A01K2217/072A01K2217/203A01K2217/206A01K2227/105A01K2267/0375C07K14/4702C12N5/0657C12N5/0662C12N2501/155C12N2501/415C12N2502/99C12N2800/30C12N2840/203C12Q1/6883C12Q2600/158G01N33/5073G01N33/56966A01K2217/052
Inventor EVANS, SYLVIACHEN, JULIN, LIZHUCHIEN, KENQYANG, YIBINGMORETTI, ALESSANDRALAUGWITZ, KARL
Owner RGT UNIV OF CALIFORNIA
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