Highly efficient devices and methods for culturing cells

Inactive Publication Date: 2008-07-24
WILSON WOLF MFG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0032]In another aspect of the present invention, structuring gas permeable devices with pla

Problems solved by technology

Thus, flasks have a large device volume relative to the amount of medium they contain.
As more and more flasks are used during culture scale up, the overall amount of space they occupy relative to the small medium volume and limited culture surface area they provide creates an inherently inefficient use of space that burdens the culture process with excess cost related to shipping, sterilization, storage, incubator space, and disposal.
This problem is compounded by the substantially increased labor and contamination risk.
Therefore, the multi-shelved flask design has an inherent potential for non-uniform culture conditions to exist throughout the device and the problem is compounded during scale up.
Thus, they quickly become unwieldy and outsize cell culture incub

Method used

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  • Highly efficient devices and methods for culturing cells
  • Highly efficient devices and methods for culturing cells
  • Highly efficient devices and methods for culturing cells

Examples

Experimental program
Comparison scheme
Effect test

Example

[0093]Example 1 and Example 2 assessed alternate geometry of the culture compartment support in order to demonstrate quantitatively how the gas permeable multi-shelf flask has the capacity to resolve the traditional flasks excessive use of shipping, sterilization, storage, incubator, and disposal space while simultaneously minimizing the potential for non-uniform culture conditions to exist.

Example

[0094]Example 3 describes how plasma charging silicone prior to gamma irradiation can limit or prevent its migration onto tissue culture treated polystyrene surfaces, thereby allowing silicone and tissue culture treated plastics to co-exist in the same culture compartment without need to deviate from standard sterilization processes.

Example 1

[0095]Culture compartment support structures for cultures with very high oxygen demand

[0096]The physical structure of a culture compartment support that would allow an improvement in islet culture, known to be one of the highest types of cultures for oxygen demand, was demonstrated by constructing a test fixture that had its lower wall comprised of a molded dimethyl silicone sheet with an average thickness measured at about 0.0072 inches thick and a surface area of 98 cm2. Gas transmission of the dimethyl silicone rubber was determined by MOCON (Minneapolis, Minn.) using their Oxtran 2 / 21 Instrument in accordance with ASTM-1927 to be about 14,30...

Example

Example 2

[0114]A different physical structure of a culture compartment support than that of Example 1 was examined in another islet culture application. In this example, test fixtures included virtually identical gas permeable material as that of Example 1. The culture compartment that supported the dimethyl silicone consisted of an open mesh in direct contact with the silicone, and a machined polycarbonate plastic sheet supported the mesh in a generally horizontal position. Unlike the culture compartment support of Example 1, the mesh resided directly upon the upper surface of the plastic sheet. The mesh geometry and material composition was identical to that of Example 1. For each cm2 of silicone membrane surface area, the volume of gas between the lower surface of the silicone and the upper surface of the plastic bottom, after displacement by the mesh, was 0.022 ml. Stated differently, the ratio of gas volume between the plastic sheet and the gas permeable membrane to the surface...

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Abstract

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

Description

RELATED APPLICATION[0001]The present application claims the benefit of U.S. Provisional Application No. 60 / 873,347 filed Dec. 7, 2006, which is incorporated herein in its entirety by reference.[0002]Each of the applications and patents cited in this text, as well as each document or reference cited in each of the applications and patents (including during the prosecution of each issued patent; “application cited documents”), and each of the PCT and foreign applications or patents corresponding to and / or claiming priority from any of these applications and patents, and each of the documents cited or referenced in each of the application cited documents, and all of the patents and co-pending applications naming John Wilson as an inventor, are hereby expressly incorporated herein by reference. More generally, documents or references are cited in this text, and, each of these documents or references (“herein-cited references”), as well as each document or reference cited in each of the ...

Claims

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Application Information

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IPC IPC(8): C12M3/06C12M3/00
CPCC12M23/08C12N5/0602C12M23/34C12M23/24
Inventor WILSON, JOHN R.WILLIAMS, JOEL
Owner WILSON WOLF MFG
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