Pcv2 immunogenic compositions and methods of producing such compositions

a technology of immunogenic compositions and compositions, applied in the direction of immunological disorders, antibody medical ingredients, peptide sources, etc., can solve the problems of ineffective vaccines, high extraction procedures, and low amount of orf2 recovered from cells, and achieve the effect of lessening this
US20090022751A1Inactive Publication Date: 2009-01-22BOEHRINGER LNGELHEIM VETMEDICA GMBH
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
BOEHRINGER LNGELHEIM VETMEDICA GMBH
Publication Date
2009-01-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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Description

RELATED APPLICATIONS

[0001] This application is a divisional of application Ser. No. 11 / 319,975, filed Dec. 29, 2005, which claims the benefit of provisional application Ser. No. 60 / 640,510, filed on Dec. 30, 2004, and application Ser. No. 11 / 034,737, filed on Jan. 13, 2005, the teachings and contents all of which are hereby incorporated by reference.SEQUENCE LISTING

[0002] This application contains a sequence listing in paper format and in computer readable format, the teachings and content of which are hereby incorporated by reference.BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] One aspect of the present invention is concerned with the recovery of a protein expressed by open reading frame 2 (ORF2) of porcine circovirus type 2 (PCV2). More particularly, the protein is a recombinant protein expressed by a transfected virus containing recombinant coding sequences for porcine circovirus type 2, open reading frame 2. Still more particularly, the transfected virus is perm...

Claims

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