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Cellomics system

a cell and system technology, applied in the field of cellomics, can solve the problems of insufficient clarification of the relationship between sensitivity and reproducibility, inability to obtain a sufficient quantity of amplification products, and inability to perform amplifying operations. to achieve the effect of low cost and high speed

Inactive Publication Date: 2009-02-12
OKANO KAZUNORI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The patent text describes a system for analyzing cells in a way that takes into account the interactions between cells in an organism. The system includes techniques for separating and culturing cells, as well as analyzing their responses. The goal is to create a system that can systemize the analysis of cells from separation to detection, and can be used for active measurement and quantitative analysis of cells. This system would provide a better understanding of cell behavior in a tissue and allow for the development of new treatments for diseases."

Problems solved by technology

The main reason for this is that the relation between the sensitivity and reproducibility has not sufficiently be clarified in the researches using DNA micro-arrays.
If the sensitivity is just insufficient, it is simply required to further amplify the mRNAs in the form of cRNAs or cDNAs, but in the quantitative analysis, sometimes amplifying operations cause errors.
Even if it is tried to analyze gene expression (including protein translation) in discrete cells in the tissue, it is impossible to obtain a sufficient quantity of amplification products from a discrete cell with the conventional amplifying operation using, for instance, a DNA micro-array, and even when amplification is forcefully performed to a level allowing for analysis with a DNA micro-array, it is impossible to obtain data reflecting the actual abundance ratio of mRNAs.
However, it has been impossible to overcome the differences between humans and other animals, now the tendency for abolishing the animal experiments has been becoming stronger.

Method used

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Examples

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example

[0285]An example is described hereinafter with identification element made up of RNA aptamer as a labeling substance to label cell surface antigen CD4 and β-phycoerythrin as a marker substance in isolating and collecting cells bonded with the RNA aptamer. That is, cells presenting the cell surface antigen CD4 is specifically labeled with the β-phycoerythrin-modified RNA aptamer as described hereinabove, and then a cell isolation chip, which is a cell sorter formed on a plastic chip substrate as disclosed in Japan Patent Application 2004-379327.

[0286]FIG. 17 is a view showing a process flow of specifically labeling a cell presenting the cell surface antigen CD4 with the β-phycobiliprotein-modified RNA aptamer and thereafter isolating the cell with a cell sorter. The top-most part of FIG. 17 shows a sample 10 with two kinds of cells 3 and 4 in a mixed manner. The cells 3 present a cell surface antigen CD4 indicated by a triangular marked out in black with a reference numeral 1. The ce...

example 6

[0350]In the examples as described above, a pipet is described in each case as it has one function, while in Example 6, an example of a pipet having two functions is described.

[0351]FIG. 27(a) is a view showing a tip of a pipet 81 having two flow paths separated by a partition plate 82, and FIG. 27(b) is a view showing configuration in which a pipet 89 is provided inside a pipet 87 to form two flow paths.

[0352]In the configuration shown in FIG. 27 (a), by making a first flow path 83 sufficiently larger than a second flow path 84, and by designing a structure in which cell suspension can be supplied from the first flow path 83 and dilution can be supplied from the second flow path 84, the pipet 11 and the pipet 20 described in Example 2 can be integrated. It is needless to say that controls of the respective flow paths are carried out with respective independent syringe pumps.

[0353]In the configuration shown in FIG. 27(b), the inner pipet 89 has an inner diameter of 50 μm, and spacin...

example 5

[0834]In order to make the twenty-first embodiment of the present invention more effective, it is preferable to eliminate a charge of probe itself and introduce a large amount of minus charge to the free terminal of the probe. For instance, PNA (Peptide Nucleic Acid) in which a phosphodiester bond of synthetic oligonucleotide is changed to a peptide bond, or CAS (Cysteine Antiesnse Compound) which includes S-carboxydimethyl-L-cysteine as a base frame may be used as a non-charged probe.

[0835]Since main chains of the polymer PNA and CAS have no charge, an electrostatic repelling force does not work with the target polynucleotide. As they have amino group and carboxyl group on the terminals thereof respectively, when an amino group is used at the fixed terminal, the fee terminal is naturally changed to a negatively charged carboxyl group. Further it is possible to make the probe act quickly in response to the electrode on the surface of the substrate by introducing a large amount of mi...

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Abstract

In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application is a continuation application of application Ser. No. 11 / 195,662 filed Aug. 3, 2005, the disclosure of which is herein incorporated by reference.FIELD OF THE INVENTION[0002]The present invention relates to promotion of researches in the field of cellomics for comprehensively understanding vital functions as those of a cell assembly, and more particularly to measurement of expression of genes in discrete cells in the state where functions of each cell structure are preserved, namely in the state where interactions between cells are preserved.BACKGROUND OF THE INVENTION[0003]With the active researches in the fields of genomics or proteomics from the last decade of 20th century up to this day, data has been accumulated concerning types and quantities of genes in various cell groups each regarded as an assembly of cells. Especially, accumulation of data concerning precise comparisons among genome sequences different from speci...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C40B30/10C40B60/12C40B40/02
CPCG01N33/5061G01N33/566A01N1/0284B01L7/50
Inventor OKANO, KAZUNORIYASUDA, KENJI
Owner OKANO KAZUNORI
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