Preparation of polymer particles
a technology of polymer particles and polymer particles, which is applied in the field of preparation of polymer particles, can solve the problems of particle particle non-uniform magnetic component distribution, difficult control of magnetic component amount and its distribution in particles, and adverse particle separation procedure performance, etc., and achieves uniform and potentially rapid response to magnetic field and large metal grain size.
Inactive Publication Date: 2010-01-14
QIAGEN GMBH
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AI Technical Summary
Benefits of technology
The present invention provides a process for preparing polymer magnetic particles by polymerizing a monomer in the presence of magnetic components. The process involves using a water phase containing magnetic components homogeneously dispersed therein and polymerizing the monomer in a water-in-oil emulsion. The resulting particles have a uniform distribution of magnetic components and can be controlled over the distribution by controlling the size of water droplets in the emulsion. The technical effect of this invention is to provide a process for preparing polymer magnetic particles with controlled magnetic component distribution.
Problems solved by technology
A problem with these methods when used to produce polymer magnetic particles is that they are multi-step methods in which the amount of magnetic component and its distribution in the particles is very difficult to control.
Generally, such particles have a non-uniform distribution of magnetic component because the magnetic component is situated only at the core of the particle.
This affects adversely performance of the particles in separation procedures.
Method used
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Experimental program
Comparison scheme
Effect test
examples
1. Particle Formation at Low pH
[0030]a)
[0031]An acidic aqueous ferrofluid (5.0 g, pH 1.4, 177 mg magnetite / g) is mixed with a parafine oil or toluene (50 g) containing 3% (by weight) Span 65. The mixture was emulsified using an ultraturax for 2 min. resulting in an W / O emulsion where the magnetite are homogenous distributed throughout the whole volume of the water droplets. The emulsion is stirred sufficient to prevent settling of magnetic droplets and TMOS (tetramethoxysilan, 9 g) in toluene is gradually added. After continuous stirring at 30° C. for 10 h, a base (0.1M NaOH or 0.5M aqueous ammonia) is added. Stirring is continued for another 3 h and the magnetic particles are isolated using a magnetic device. The polymer magnetic particles are subsequently washed with toluene, methanol and water. The particles may be used e.g. for isolating DNA directly or a drying step followed by a re-suspension in water can be included.
b)
[0032]While stirring at 0° C., TMOS (9 g) is added to an a...
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Abstract
A process for the preparation of polymer magnetic particles, which comprises: (a) providing a water phase containing magnetic components homogeneously dispersed therein; (b) wherein the water phase is contacted with or further contains a polymerisable metal-containing or organic monomer which is soluble in the water phase; and (c) polymerizing the monomer in the presence of the magnetic in which the magnetic components are substantially uniformly distributed; wherein at least a part of the polymerizing step (c) is carried out in a water-in-oil emulsion in which the water phase containing the magnetic components homogeneously dispersed therein is present as a discontinuous phase in a continuous oil phase.
Description
[0001]The invention relates to a process for the preparation of polymer magnetic particles and particles obtainable therefrom, particularly for use as separation media, especially for separating a target substance from a sample such as a biological sample.BACKGROUND TO THE INVENTION[0002]Various types of separation media are available for use in isolating target substances in chemical or biological samples. In molecular biology, various materials are used in fractionation procedures, including agarose and polyacrylamide in electrophoretic fractionation, and gel permeation, ion exchange and affinity materials for chromatography. Among such materials, both organic polymer and silicon-based particles find use in separations of targets such as nucleic acids. In each type of separation there is a need at some point physically to remove a liquid phase from the particles in the solid phase. This may be achieved by immobilising the particles, for example in the form of a column or on a gel ...
Claims
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IPC IPC(8): H01F1/26B01J20/26B01J20/30C08F2/32C08F2/44
CPCB01J20/26B01J20/28009Y10T428/254C08F2/32C08F2/44B01J20/30
Inventor SKAGESTAD, VIDARKILAAS, LARS
Owner QIAGEN GMBH