Methods for using soy peptides to inhibit h3 acetylation, reduce expression of hmg-coa reductase and increase ldl receptor and sp1 expression in a mammal

a technology of soy peptides and h3 acetylation, which is applied in the field of peptides, can solve the problems of no known effective method of safely inhibiting h3 acetylation, and achieve the effects of inhibiting h3 acetylation, increasing ldl receptor and sp1 expression, and reducing expression of hmg-coa reductas

Inactive Publication Date: 2010-08-05
S L TECH
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Benefits of technology

[0051]The present invention relates generally to a class of peptides that provide mammals with a variety of health related benefits. More specifically, the present invention involves using soy peptides to inhibit H3 acetylation, reduce express...

Problems solved by technology

Unfortunately, presently there are no known effective methods of safely inhibiting H3 acetylatio...

Method used

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  • Methods for using soy peptides to inhibit h3 acetylation, reduce expression of hmg-coa reductase and increase ldl receptor and sp1 expression in a mammal
  • Methods for using soy peptides to inhibit h3 acetylation, reduce expression of hmg-coa reductase and increase ldl receptor and sp1 expression in a mammal
  • Methods for using soy peptides to inhibit h3 acetylation, reduce expression of hmg-coa reductase and increase ldl receptor and sp1 expression in a mammal

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example 1

Lunasin Reduces Expression of HMG-CoA Reductase, Increases Expression of LDL Receptor

[0120]The lowering of serum cholesterol by statin drugs is achieved by competitively inhibiting the HMG-CoA reductase, the rate limiting enzyme in the body's metabolic pathway for synthesis of cholesterol. By reducing endogenous cholesterol synthesis, statins also cause liver cells to up regulate expression of the LDL receptor, leading to increased clearance of low-density lipoprotein (LDL) from the bloodstream (9). In 1985, Michael Brown and Joseph Goldstein received the Nobel Prize in Medicine for their work in clarifying this LDL-lowering mechanism.

[0121]Transcriptional regulation of HMG-CoA reductase and LDL receptor is controlled by the Sterol Regulatory Element-Binding Protein-1 and -2 (SREBP). This protein binds to the sterol regulatory element (SRE) located on the 5′ end of the reductase and the LDL receptor genes. When SREBP is inactive, it is bound to the ER or nuclear membrane. When chole...

example 2

Lunasin's Effect on Expression of Sp1 Coactivator

[0128]Unlike HMG-CoA reductase, SREBP activation of LDL-receptor by sterol depletion requires increased recruitment of Sp1 co-activator to a site adjacent to SREBP in the promoter / regulatory sequence of LDL-receptor gene (25). As shown in FIG. 3, the up regulation of LDL-receptor by lunasin (LS) in cholesterol-free media may be due to increased availability and recruitment of the Sp1 coactivator to the LDL-receptor promoter / regulatory sequence. To test this hypothesis, the level of Sp1 was determined in lunasin-treated growth media and cholesterol-free media by Western analysis using Sp1 antibody, as follows: HepG2 cells (1×106) were grown from confluence in DMEM with 10% FBS for 24 hours before growth media was replaced with fresh growth media or cholesterol-free media (to activate SREBP) and treated with, or without 10 uM synthetic lunasin. After 24 hours, total protein was extracted from each treatment and 10 ug protein loaded onto...

example 3

[0133]Lunasin can be extracted from commercial sources of soy protein. Lunasin has been found in significant amounts from commercial sources of soy protein and its homologues from other seed sources such as barley and wheat. To identify preferred sources for the starting raw material that can be used for lunasin extraction, several commercially available soy protein products were screened for the presence of lunasin.

[0134]The procedure used was as follows: approximately 500 mg of soy protein samples obtained from different commercial sources (Solae, St. Louis, Mo.) were dissolved in 50 mL of aqueous phosphate buffer (pH 7.2) by shaking for 1 hour at room temperature. Samples were centrifuged at 2500 rpm for 30 minutes and the aqueous fraction separated and put in separate tubes. Protein concentrations were measured by Bradford assay and around 20 ug of total protein were loaded onto two Bio-Rad Laboratories (Hercules, Calif.) 16% Tris-Tricine gels. One of the SDS-PAGE gels (I) was s...

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Abstract

Controlled studies demonstrate that methods using soy related peptides inhibit H3 acetylation, reduce expression of HMG-CoA reductase and increase LDL receptor and Sp1 expression in mammals. The present disclosure is generally directed to using lunasin peptides and/or lunasin peptide derivatives to 1) inhibit H3 acetylation, 2) reduce expression of HMG-CoA reductase, 3) increase LDL receptor expression or 4) increase Sp1 expression in a mammal. In at least one exemplary embodiment of the present disclosure, an effective amount of lunasin peptides or lunasin peptide derivatives and one or more enzyme inhibitors is provided to a mammal to 1) inhibit H3 acetylation, 2) reduce expression of HMG-CoA reductase, 3) increase LDL receptor expression or 4) increase Sp1 expression in a mammal. Additionally, lunasin will protect against, prevent, or reduce: 1) the expression of Matrix metalloproteinase (MMP-1), 2) collagen breakdown, 3) photoaging and 4) the formation of skin wrinkles.

Description

RELATED APPLICATIONS[0001]This application claims the benefit of priority to U.S. patent application Ser. No. 11 / 532,528 filed Sep. 16, 2006. This application also claims the benefit of priority to U.S. patent application Ser. No. 12 / 441,384, filed Mar. 14, 2009, which claims priority to International Application Number PCT / US07 / 78584, filed Sep. 15, 2007, which claims priority to U.S. provisional No. 60 / 966,529, filed Sep. 16, 2006 (formerly U.S. patent application Ser. No. 11 / 532,526, filed Sep. 16, 2006) and claims priority to U.S. provisional No. 61 / 007,925, filed Jul. 17, 2007 (formerly U.S. patent application Ser. No. 11 / 879,249, filed Jul. 17, 2007. All of the above listed applications are hereby incorporated by reference herein in their entireties.BACKGROUND[0002]1. Technical Field[0003]This disclosure relates generally to a class of peptides that provide mammals with a variety of health related benefits. More specifically, the present disclosure related to using soy peptide...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61Q19/08A61Q19/00A23L11/00
CPCA61K38/168A61K38/005C07K14/415A61K45/06A61K36/48A23L11/05A61P17/00A61P3/06A61P43/00A61P9/00A61P9/10A61P3/10A23V2002/00A61K9/0053
Inventor GALVEZ, ALFREDO FLORES
Owner S L TECH
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