Novel leuconostoc citreum and fermented foods using the same as a starter, and compositions thereof
a technology of leuconostoc citreum and fermented foods, which is applied in the field of leuconostoc citreum bacteria and fermented foods using the bacteria as a starter, and compositions thereof. it can solve the problems of kimchi manufacturing, low raw material quality, and the change of kimchi quality to achieve constant quality
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example 1
Deducting Improvement Aspects by Monitoring Kimchi Quality Change During a Year and Isolating GN34 Bacteria
[0044]Quality change of Kimchi was monitored during a year in order to find the causes of quality change and to improve the quality.
[0045]Kimchi manufactured according to the manufacture method of CJ CheilJedang was used. The Kimchi manufactured between March and May, 15 was classified as spring Kimchi; that manufactured between May, 16 and August as summer Kimchi; that manufactured between September and November 15 as autumn Kimchi; and that manufactured between November 16 and February as winter Kimchi.
[0046]As a result of analyzing the taste quality of the Kimchi manufactured in each season in a sensory test by 5-point scale, it was found that the summer Kimchi has a low value of about 3.5 for a general taste item, whereas the Kimchi manufactured in the other season has an average range of 3.8 to 4.0. The above results are shown in the FIG. 1.
[0047]In order to analyze the fe...
example 2
Isolation and Identification of Microorganism Leuconostoc citreum CJGN34 Bacteria
[0051]In the above Example 1, the causes responsible for the quality change of Kimchi during a year were analyzed and lactic acid bacteria were isolated from the well-aged delicious winter Kimchi. The lactic acid bacteria were named as Leuconostoc citreum CJGN34. Lactic acid bacteria Leuconostoc citreum CJGN34 were streaked onto solid MRS medium (Difco, USA) containing 1.5% agar and incubated at 30° C. for 24 hrs. Colonies confirmed as being purely separated were taken by a loop and incubated with MRS broth (Difco, USA) at 30° C. for 18 to 24 hrs.
[0052]Then, the morphology and physiological properties of Leuconostoc citreum CJGN34 bacteria were determined with API50CH and API50CHL kits (Bio-Me'reux) according to the methods disclosed in Kim et. al., Leuconostoc inhae sp. nov., a lactic acid bacterium isolated from kimchi, International Journal of Systematic and Evolutional Microbiology, Volume 53, July ...
example 3
Safety Assessment of Leuconostoc citreum CJGN34
[0055]Hemolysis test, gelatin liquefaction test, hazardous metabolite (ammonification) test and phenylalanine deaminase test were performed according to the safety assessment methods suggested by the standard of Korea Biotechnology Industry Organization to assess safety of the bacteria isolated from the Example 1. The obtained result is summarized in table 3.
TABLE 3Safety assessment for Leuconostoc citreum CJGN34testsgelatinphenylalanine bacterialiquefactiondeaminasehemolysisammonificationCJGN34negativenegativenegativenegative
[0056]Based on the above result, Leuconostoc citreum CJGN34 was found negative in all of the gelatin liquefaction test, hazardoug metabolite (ammonification) test, phenylalanine deaminase test and hemolysis test. Accordingly, Leuconostoc citreum CJGN34 was confirmed as being safe for administration to a human being.
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