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Methods of preservation

a technology of preservation and methods, applied in the field of preservation methods, can solve the problems of reducing the anti-microbial performance of the body and reducing the effect of anti-microbial performan

Inactive Publication Date: 2013-05-30
SAWYER ANTHONY JOSEPH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides methods for preserving food and other products using environmentally safe compositions that have antimicrobial, antibacterial, antiviral, and antifungal properties. The methods involve the use of GRAS materials that are safe and efficacious, and will provide long-lasting lethality to microorganisms found in such products. The invention also provides packaging materials that incorporate the biocidal compositions, which can effectively preserve packaged food products. The more water soluble salts of the biocidal compounds have been found to provide superior preservation compared to the limited solubility salts.

Problems solved by technology

When only one of these three agents was used, the anti-microbial performance was considerably reduced.

Method used

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  • Methods of preservation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030]Synergistic preservative action of Nα-lauroyl arginine ethyl ester (LAE). The results are set forth below in Table 1.

TABLE 1The synergistic preservative action of Nα-lauroyl arginineethyl ester (LAE) with glycerol monolaurateLAE ppmMonolaurin ppm120100806040302015107.50000000GGGG100000000GGG2000000000GG3000000000GG40000000000G5000000000GG6000000000GG7000000000GG8000000PTPTGGG900000PTPTPTGGG1000000PTPTPTGGG

[0031]The results in Table 1 clearly indicate synergy between LAE and glycerol monolaurate (“monolaurin”) as a combination preservative. Thus used in the amounts of 10 ppm LAE with 40 ppm Monolaurin, complete growth inhibition of candida albicans is obtained whereas when used alone, 30 ppm of LAE or much more than 100 ppm monolaurin would be required to achieve growth inhibition.

example 2

[0032]Time Kill Test. The results are set forth below in Table 2.

TABLE 2Time Kill Test at 90 seconds with a LAE concentration at 500 ppmand ML concentration at 45 ppm.OrganismLog ReductionKlebsiella pneumoniae>2.0Pseudomonas aeruginosa>2.0

[0033]The method of preservation of this invention can be used by incorporating the biocidal salts in equipment for handling blood products such as blood sera, test tubes, vacutainer tubes, and other blood handling items; plasma bags; disposable gloves; condoms; band-aids, or bandages; and feminine hygiene products including but not limited to sanitary napkins and tampons.

[0034]The methods of preservation of this invention comprise utilizing a salt of a cation of a Nα—(C1-C22) alkanoyl di-basic amino acid alkyl (C1-C22) ester cationic biocidal molecule and an anion consisting of acetate, sulphate, chloride, or bromide.

[0035]Preferably, the di-basic amino acid of the first component is selected from the group consisting of arginine, lysine, histidin...

example 3

Antimicrobial Activity of Compounded Films

[0044]Two beef loins from a slaughterhouse were tested in a heat-sealed package simulating food packaging. The linear low density polyethylene film was compounded with 1% by weight LAE-HCl biocidal salt based on the weight of the film. The results are reported in Table 3 below. The log colony forming units (“CFU”) reduction values for bacteria that are part of the background flora of raw meat for both samples are 42 days indicate that there is a release of the salt of the invention from the polyethylene film to the surrounding beef surfaces.

TABLE 3Antimicrobial Activity of Compounded FilmsDay 42LogDay 42Sample IDLog CFU / gReductionDay 42% ReductionDays714212842Vs. ControlCFU / gVs. Control oin 1 film2.824.685.637.067.24017,278,6870(control) oin 2 film2.313.683.975.325.571.67371,28297.91% LAE HCl indicates data missing or illegible when filed

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Abstract

This invention discloses a method of preservation of a food product comprising the step of adding 1) a first component comprising between 10 ppm and 1% of a biocidal salt of Nα—(C1-C22) alkanoyl di-basic amino acid alkyl (C1-C22) ester cationic biocidal molecule with an anionic counterion, and 2) a second component comprising from 10 ppm to 1% by weight an acyl monoglyceride, directly to a food product. The preferred cationic biocidal molecule comprises Nα-lauroyl-L-arginine ethyl ester (“LAE”). The invention also discloses the method of preservation of a food product using salts of a Nα—(C1-C22) alkanoyl di-basic amino acid alkyl (C1-C22) ester cationic biocidal molecule and an anionic counterion with or without a monoglyceride of a fatty acid, whereby the packaging film is compounded with the salts and the optional monoglyceride of a fatty acid.

Description

REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of application Ser. No. 12 / 455,197, filed May 28, 2009. This application claims the benefit of provisional application Ser. No. 61 / 341,702, filed Apr. 3, 2010. The disclosures of all of the foregoing applications are incorporated by reference herein in their entirety.FIELD OF THE INVENTION[0002]The invention relates to a method of preservation using biocidal compositions that are environmentally safe that can have antimicrobial, antibacterial, antiviral, and antifungal properties. The method of preservation uses salts of a cation of a INV (C1-C22) alkanoyl di-basic amino acid alkyl (C1-C22) ester biocidal molecule and an anion of a monomeric molecule with or without a monoglyceride of a fatty acid.BACKGROUND OF THE INVENTION[0003]The use of environmentally beneficial compositions, especially those that are referred to as “green”, is important in choosing biocidal materials that can be used as preservatives. T...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23L3/3544C07C229/00C07D233/54C07D209/04A23L3/3526C07C229/26
CPCA23L3/3508A23L3/3526C07D209/04C07D233/54A23L3/3544
Inventor SAWYER, ANTHONY JOSEPHSTOCKEL, RICHARD F.
Owner SAWYER ANTHONY JOSEPH
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