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Processing biomass

a technology of biomass and saccharification, applied in the field of methods, can solve the problems of low yield of naturally occurring cellulosic materials, low yield of biomass, and high cost, and achieve the effect of increasing the efficiency of saccharification and avoiding negative feedback inhibition of enzymatic reactions

Inactive Publication Date: 2014-02-06
XYLECO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach enhances the efficiency of saccharification and fermentation, allowing for higher yields and purer product production, overcoming the limitations of existing methods by facilitating better access to enzymes and reducing the toxicity effects of solvents on microorganisms.

Problems solved by technology

This compact matrix is difficult to access by enzymes and other chemical, biochemical and biological processes.
Cellulosic biomass materials (e.g., biomass material from which substantially all the lignin has been removed) can be more accessible to enzymes and other conversion processes, but even so, naturally-occurring cellulosic materials often have low yields (relative to theoretical yields) when contacted with hydrolyzing enzymes.
While a number of methods have been tried to extract structural carbohydrates from lignocellulosic biomass, they are either too expensive, produce too low a yield, leave undesirable chemicals in the resulting product, or simply degrade the sugars.

Method used

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  • Processing biomass
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Examples

Experimental program
Comparison scheme
Effect test

example 1

Butanol Production on Glucose, Xylose and Fructose

[0171]A P2 based medium as described in U.S. Pat. No. 6,358,717 was used for the following tests. The medium was composed of the following separately prepared solutions (in grams per 100 ml of distilled water, unless indicated otherwise): sugar (see below for types and amounts), 790 ml of distilled water (solution I), 0.5 g of K2HPO4, 0.5 g of KH2PO4, 2.2 g of CH3COONH4 (solution II), 2.0 g of MgSO4.7H2O, 0.1 g of MnSO4.H2O, 0.1 g of NaCl, 0.1 g of FeSO4.7H2O (solution III), and 100 mg of p-aminobenzoic acid, 100 mg of thiamine, 1 mg of biotin (solution IV). Solutions I and II were filter sterilized and subsequently mixed to form a sugar buffer solution. Solutions III and IV were filter sterilized. Portions (10 and 1 ml) of solutions III and IV, respectively, were added aseptically to the sugar-buffer solution. The final pH of the P2 medium was 6.6.

[0172]The amounts of sugars used were: for medium GXP2, 43 g glucose and 24 g xylose; ...

example 2

Butanol Production on Fructose Vs. Glucose & Xylose

[0174]Ten ml of P2 media containing either a glucose / xylose mixture of fructose alone (32 g / L) were incubated at 30° C. with one of either Clostridium saccharoperbutylacetonium ATCC strain 27021 or 27022. As in Example 1, the results presented in the table below show that more butanol is generated when Clostridium is grown on fructose, as opposed to glucose or xylose.

TABLE 3Clostridium vial growth on fructose or glucose / xyloseas the carbon source.ButanolTimepointStrainSubstrateProduction (g / L)(hrs)ATCC 27021Fructose11.748ATCC 27021Glucose / Xylose2.348ATCC 27022Fructose11.696ATCC 27022Glucose / Xylose4.096

[0175]Any patent, publication, or other disclosure material, in whole or in part, that is said to be incorporated by reference herein is incorporated herein only to the extent that the incorporated material does not conflict with existing definitions, statements, or other disclosure material set forth in this disclosure. As such, and t...

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Abstract

Fructose, e.g., fructose derived from a cellulosic or lignocellulosic material, is use, e.g., fermented to produce a product, e.g., a solvent.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a continuation of PCT / US2012 / 071097, filed Dec. 20, 2012, which claimed priority from U.S. Provisional Application Nos. 61 / 579,552 and 61 / 579,559 both filed on Dec. 22, 2011. The entire disclosures of the above applications are incorporated herein by reference.FIELD OF THE INVENTION[0002]The invention pertains to methods of converting biomass to useful products. In particular, the inventions relates to the production of products, such as butanol from sugars, such as fructose.BACKGROUND[0003]As demand for petroleum increases, so too does interest in renewable feedstocks for manufacturing biofuels and biochemicals. The use of lignocellulosic biomass as a feedstock for such manufacturing processes has been studied since the 1970s. Lignocellulosic biomass is attractive because it is abundant, renewable, domestically produced, and does not compete with food industry uses.[0004]Many potential lignocellulosic feedstocks are av...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12P7/16
CPCC12P7/16B01J19/08C12N1/16C12N9/90C12P7/10C12P19/02C12P19/24Y02E50/10C12P19/14C12P2201/00
Inventor MEDOFF, MARSHALLMASTERMAN, THOMAS CRAIGFINN, MICHAEL W.
Owner XYLECO INC