Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate

Abstract

Disclosed herein are methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, that have been seeded onto and cultured on a substrate, such as a polymeric substrate. Such cryopreserved stem cells are useful for cell therapies, such as treatment of ocular damage or disease.

Description

RELATED CASES[0001]This application claims the benefit of U.S. Provisional Application Ser. No. 61 / 481,015, filed on Apr. 29, 2011, the contents of which is expressly incorporated in its entirety by reference herein.BACKGROUND[0002]1. Field of the Invention[0003]The present application relates generally to methods and compositions for the cryopreservation of stem cells grown on a substrate. In particular, methods and compositions for the cryopreservation of retinal pigment epithelial (RPE) cells grown on a polymeric substrate.[0004]2. Description of the Related Art[0005]The scope of human disease that involves loss of or damage to cells is vast and includes, but is not limited to, ocular disease, neurodegenerative disease, endocrine diseases, cancers, and cardiovascular disease. Cellular therapy involves the use of cells, and in some cases stem cells, to treat diseased or damaged tissues. It is rapidly coming to the forefront of technologies that are poised to treat many diseases, i...

AI Technical Summary

Benefits of technology

The patent is about a method for cryopreserving stem cells on a substrate. The method involves reducing the temperature of the substrate and the cells quickly, while maintaining the viability of the cells. The substrate is made of a special material called parylene, which has been treated to enhance cell adhesion. The method also involves adjusting the temperature reduction profile to improve the response of the cells to the temperature change. The technical effects of this method include better long-term storage and function of the stem cells after thawing.

Problems solved by technology

Numerous pathologies can compromise or entirely eliminate an individual's ability to perceive visual images, including trauma to the eye, infection, degeneration, vascular irregularities, and inflammatory problems.

The function of the macula may be adversely affected by age related macular degeneration (wet or dry), diabetic macular edema, idiopathic choroidal neovascularization, high myopia macular degeneration, or advanced retinitis pigmentosa, among other pathologies.

Age related macular degeneration typically causes a loss of vision in the center of the visual field (the macula) because of damage to the retina.

It is a major cause of visual impairment in older adults (>50 years).

In the dry form, cellular debris (drusen) accumulates between the retina and the choroid, which puts pressure on the retina, possibly leading to retinal detachment and loss of vision.

In the more severe wet form, newly formed blood vessels from the choroid infiltrate the space behind the macula, which causes death of photoreceptors and their supporting cells.

In conjunction with the loss of functional cells in the eye, the newly formed blood vessels are fragile and often leak blood and interstitial fluid, which can further damage the macula.

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