Cysteine engineered antibodies and conjugates

a technology of engineered antibodies and conjugates, applied in the field of antibodies, can solve the problems of potential problems such as the inability to engineer in cysteine thiol groups by the mutation of various amino acid residues of a protein to cysteine amino acids, and the inability to separate analytical and preparative methods,
US20140288280A1Inactive Publication Date: 2014-09-25GENENTECH INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
GENENTECH INC
Publication Date
2014-09-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody. Certain highly reactive cysteine engineered antibodies were identified by the PHESELECTOR assay. Isolated cysteine engineered antibodies may be covalently attached to a capture label, a detection label, a drug moiety, or a solid support.
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Description

REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. patent application Ser. No. 13 / 154,672, filed 7 Jun. 2011, which claims the benefit under 35 USC §119(e) of U.S. Provisional Application Ser. No. 61 / 352,728 filed on 8 Jun. 2010, each of which is incorporated by reference in their entireties.FIELD OF THE INVENTION

[0002] The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions.BACKGROUND OF THE INVENTION

[0003] Antibody drug conjugates (ADC) are attractive targeted chemo-the...

Claims

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