Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sample preparation, processing and analysis systems

a sample preparation and analysis system technology, applied in the field of sample preparation, processing and analysis systems, can solve the problems of large sample preparation and analysis system, difficult to transport without substantial effort, and various limitations of the methods and systems currently available for dna profiling, so as to achieve the effect of reducing the size of the sample, reducing the cost, and reducing the cost of processing

Inactive Publication Date: 2015-05-21
INTEGENX
View PDF2 Cites 60 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent discusses the need for improved systems and methods for genetic profiling that have a smaller footprint, are faster, and can be done at a lower cost with less skilled operators. The systems can be fully integrated, automated, and portable, allowing for on-the-go use in remote locations or situations where transportation is not readily available. The control module can include a user interface and sensors to detect and measure operation and transmit the information to a computer for analysis and adjustment of the instrument if needed. Overall, this patent describes a technological solution that improves the efficiency and flexibility of genetic profiling for research and personalized medicine applications.

Problems solved by technology

Sample preparation is a ubiquitous problem in biological analytical systems.
Methods and systems currently available for DNA profiling have various limitations.
For instance, sample preparation and analysis systems for DNA profiling are typically bulky and difficult to transport without substantial effort.
This makes the use of such systems in the field, such as on a battlefield, difficult and impractical.
In addition, current systems and methods are expensive to use and maintain, and genetic profiles take a substantially large amount of time to prepare.
This is unappealing in cases in which time is of the essence, such as cases in which law enforcement officials are in pursuit of a suspect or can detain a suspect for only a limited period of time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sample preparation, processing and analysis systems
  • Sample preparation, processing and analysis systems
  • Sample preparation, processing and analysis systems

Examples

Experimental program
Comparison scheme
Effect test

embodiment 1

2. The cassette of embodiment 1, further comprising a port that brings an individual chamber of said plurality of closed and fluidically isolated chambers in fluid communication with a pressure source.

3. The cassette of embodiment 1 or 2, wherein said fluid flow path is a multidirectional fluid flow path

4. The cassette of any one of the preceding embodiments, wherein said plurality of puncturing elements include openings that are in fluid communication with the microfluidic channel.

5. The cassette of any one of the preceding embodiments, wherein the microfluidic channel comprises one or more selectably closable channels.

6. The cassette of any one of the preceding embodiments, wherein the microfluidic channel is in fluid communication with one or more valves.

embodiment 6

7. The cassette of embodiment 6, wherein the one or more valves are diaphragm valves.

8. The cassette of embodiment 6 or 7, wherein the one or more valves are pneumatically actuated valves.

9. The cassette of embodiment 6 or 7, wherein the one or more valves are piezoelectric valves.

10. The cassette of any one of the preceding embodiments, wherein:

[0364]the microfluidic channel comprises a plurality of microfluidic channels;

[0365]at least one, or each, of the plurality of chambers comprises a first port connectable to a microfluidic channel and a second port connectable to a microfluidic channel; and

[0366]engaging the microfluidic device with the container creates a fluid flow path comprising a microfluidic channel connected to the first port of a chamber, the chamber, and a microfluidic channel connected to the second port of the chamber.

11. The cassette of any one of the preceding embodiments, wherein said plurality of closed and fluidically isolated chambers comprise microfluidic c...

embodiment 13

14. The cassette of embodiment 13, wherein said thermal cycler assembly is configured for polymerase chain reaction (PCR).

15. The cassette of embodiment 13 or 14, wherein said thermal cycler assembly comprises a thermal cycling chamber.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Pressureaaaaaaaaaa
Currentaaaaaaaaaa
Login to View More

Abstract

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Description

CROSS-REFERENCE[0001]This application is a continuation patent application of U.S. patent application Ser. No. 13 / 656,503, filed Oct. 19, 2012, now U.S. Pat. No. 8,894,946, which claims the benefit of and priority to U.S. Provisional Patent Application Ser. No. 61 / 550,364, filed Oct. 21, 2011; U.S. Provisional Patent Application Ser. No. 61 / 605,169, filed Feb. 29, 2012; U.S. Provisional Patent Application Ser. No. 61 / 610,977, filed Mar. 14, 2012; U.S. Provisional Patent Application Ser. No. 61 / 641,120, filed May 1, 2012; U.S. Provisional Patent Application Ser. No. 61 / 654,749, filed Jun. 1, 2012; U.S. Provisional Patent Application Ser. No. 61 / 664,726, filed Jun. 26, 2012; U.S. Provisional Patent Application Ser. No. 61 / 671,592, filed Jul. 13, 2012; U.S. Provisional Patent Application Ser. No. 61 / 674,295, filed Jul. 20, 2012; U.S. Provisional Patent Application Ser. No. 61 / 691,242, filed Aug. 20, 2012; U.S. Provisional Patent Application Ser. No. 61 / 696,809, filed Sep. 5, 2012; and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/447G01V3/00C12Q1/68G01V8/10B01L3/00B01L9/00
CPCG01N27/447B01L3/502715B01L9/00B01L2400/0421G01V8/10G01V3/00C12Q1/6844G01N27/44743G01N27/44791B01L3/5029B01L3/527B01L7/52B01L2200/025B01L2200/028B01L2200/04B01L2200/0647B01L2200/143B01L2300/023B01L2300/027B01L2300/0672B01L2300/123B01L2400/043B01L2400/0478B01L2400/0616G01N2035/00158G01N2035/0091B01F33/30B01F33/452B01L2400/0487B01L2400/0638
Inventor NIELSEN, WILLIAM D.BELCINSKI, RICHARD J.BOGDAN, GREGORYEBERHART, DAVIDEL-SISSI, OMARJOVANOVICH, STEVAN B.RECKNOR, MICHAELVAN GELDER, EZRAWYRICK, DAVID W.
Owner INTEGENX
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com