Methods for producing streptococcus pneumoniae capsular polysaccharide carrier protein conjugates

a technology of capsular polysaccharide and pneumococcal polysaccharide, which is applied in the direction of pharmaceutical delivery mechanism, inorganic non-active ingredients, antibody medical ingredients, etc., can solve the problems of lyophilization preclude the ability to optimize individual formulations and cycle parameters, and the complication of pneumococcal diseases can be significant, etc., to achieve the ability to optimize individual formulations and cycle parameters, and facilitate handling
US20210252125A1Inactive Publication Date: 2021-08-19MERCK SHARP & DOHME LLC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
MERCK SHARP & DOHME LLC
Publication Date
2021-08-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.
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Description

BACKGROUND OF THE INVENTION(1) Field of the Invention

[0001] The present invention relates to a method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, wherein the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.(2) Description of Related Art

[0002] Streptococcus pneumoniae, one example of an encapsulated bacterium, is a significant cause of serious disease world-wid...

Claims

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