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Immunostimulatory, covalently lipidated oligonucleotides

a covalently lipidated, oligonucleotide technology, applied in the field of immunosuppression, can solve the problems of backbone reducing the ability of the oligonucleotide to form a stable duplex structure, further experimentation with 3′ lipophilic modifications, reducing the stability of the duplex, etc., to facilitate the penetration of the oligonucleotide

Inactive Publication Date: 2010-06-22
ONCOTHYREON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention relates to a method of stimulating the immune system by administering an oligonucleotide containing one or more \"CxG\" dinucleotide units (which are present in bacteria and have immunostimulatory activity) and one or more lipophilic groups. The lipophilic group can help the oligonucleotide penetrate cell membranes and interact with cell surface receptors. The oligonucleotide can also have alternative backbone structures and can be administered to individuals with cancer or other immune system disorders. The invention also includes certain types of lipidated oligonucleotides with different numbers of nucleotides and containing multiple \"CxG\" units."

Problems solved by technology

However, they found that the change in backbone reduced the ability of the oligonucleotide to form a stable duplex structure.
Thus, Cheng et al. discouraged further experimentation with 3′ lipophilic modifications other than those with the steroid skeleton of his Formula 1.
Indeed, since GNA backbones reduce duplex stability, use of a GNA backbone would have been contrary to Cheng et al.

Method used

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  • Immunostimulatory, covalently lipidated oligonucleotides

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Compound 8

[0515]To a solution of 1,2-dodecandiol (5.0 g, 24.7 mmol) and DIPEA (3.86 g, 5.2 mL, 27.7 mmol) in DCM / THF (10:1, 500 mL) was slowly added DMT-Cl solution (0.2 g, 27.2 mmol, in 100 mL DCM) over a period of 2 h at 0° C. The mixture was stirred at 0° C. for 3 h, followed by usual aqueous work-up. The product was purified by flash chromatography (hexane / ethyl acetate, 3:1) to give 8 (6.5 g, 52%). C33H44O4 (504.44). TLC: Rf=0.67 (hexane / ethyl acetate, 2:1). 1H NMR (300 MHz, CDCl3): δ 0.88 (t, J=6.5 Hz, 3 H), 1.24 (br s, 16 H), 1.37 (m, 2 H), 2.32 (d, J=3.5 Hz, 1 H, OH), 3.00 (dd, J=9.0, 7.0 Hz, 1 H), 3.16 (dd, J=9.0, 3.5 Hz, 1 H), 3.77 (m, 1 H), 3.79 (s, 6 H, 2 OCH3), 6.85-7.60 (m, 13 H, Ar—H).

example 2

Preparation of Modified CPG Resin 9

[0516]Controlled pore glass (CPG) resin modified with long-chain-amino-alkyl group (lcaa-CPG) (0.6 g, 57 μmol) was treated with succinic andydride (57 mg, 0.57 mmol) in DMF (1.5 mL) overnight. Solvent was drained out and the resin washed with DMF (×5). The process was repeated once. The unreacted free amine on the resin was capped by treatment with NMI / Ac2O / THF (1:1:8, v / v / v) for 5 min. The resin was then thoroughly washed with THF (×5) and CH3CN (×5), dried under high vacuum to give the succinic acid-attached support.

[0517]The succinic acid-attached CPG resin (0.3 g, ˜28.5 mmol) was suspended in THF (2 mL) and 8 (43 mg, 85.5 μmol), DIC (14 μL), and DMAP (cat.) were added. The mixture was stirred at room temperature for 12 h. the solvent was drained out and the reaction was repeated once. The unreacted free carboxylic acid was capped by treating with methanol / DIC / DMAP for 3 h. The resin was then thoroughly washed with THF (×5) and DCM (×5), dried u...

example 3

Preparation of Compound 1-5

a) De-tritylation

[0518]Lipid-modified CPG resin 9 (0.3 g, 28.5 μmol) was treated with trichloroacetic acid (TCA) (1% in DCM, w / v) for 5 min to remove the DMT-group. The process was repeated once for 1 min, followed by thorough wash with DCM (×5), acetonitrile (×5) and THF (×3).

b) Coupling

[0519]The resin was then swelled in dry THF (2 mL), and phosphoramidite reagent (42.8 μmol, 1.5 eq.) and tetrazole (3.0 mg, 42.8 mol) were added. The mixture was bubbled with N2 gas for 16 h. After the removal of solvent and washing of the resin, the coupling with phosphoramidite was repeated once.

c) Capping

[0520]The unreacted free hydroxyl group on the resin was capped by treating with NMI / Ac2O / THF (1:1:8, v / v / v) for 5 min. followed by thorough wash with DCM, acetonitrile and THF.

d) Oxidation

[0521]The resin was then treated with 2-butanone peroxide (, 2.0 mL, 0.1 M in DCM) for 5 min. and washed thoroughly with DCM, acetonitrile, and THF.

[0522]The synthesis was continued b...

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PUM

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Abstract

Covalently lipidated oligonucleotides comprising the CpG dinucleotide unit, or an analogue thereof, may be used as immunostimulatory agents to protect against a disease caused by a cancer cell or a pathogen, either alone or in conjunction with immunogens and / or non-immunological agents.Lipidated oligonucleotides with special backbones, lipidated oligonucleotides with fewer than eight nucleotides, and lipidated oligonucleotides comprising a plurality of CpG dinucleotide-containing segments connected by a long internucleoside linkage are of particular interest. These compounds are also novel per se.

Description

[0001]This application is a nonprovisional of U.S. provisional appl. 60 / 353,195 filed Feb. 4, 2002, which is hereby incorporated by reference in its entirety.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]This invention relates to the use of covalently lipidated oligonucleotides comprising the CpG dinucleotide unit, or an analogue thereof, as immunostimulatory agents. Lipidated oligonucleotides with special backbones, lipidated oligonucleotides with fewer than eight nucleotides, and lipidated oligonucleotides comprising a plurality of CpG dinucleotide-containing segments connected by a long internucleoside linkage are of particular interest.[0004]2. Description of the Background Art[0005]Effective host defense against invading microorganisms requires the detection of foreign pathogens and the rapid deployment of an antimicrobial effector response (Krutzik et al, 2001). Indeed, the innate immune system detects the presence and the nature of the infection by recognizi...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A01N43/04A61K39/38A61K39/00C12N15/09A61K39/002A61K39/02A61K39/12A61K39/39A61P31/00A61P33/00A61P35/00A61P37/00A61P37/04C07H21/00C07H21/02C07H21/04C07K7/00
CPCA61K39/0011A61K39/39C07H21/00A61K2039/55561A61K2039/55555A61P31/00A61P33/00A61P35/00A61P37/00A61P37/04
Inventor JIANG, ZI-HUAKOGANTY, RAOYALAMATI, DAMAYANTHIBAEK, MYUNG-GI
Owner ONCOTHYREON
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