Method for inducing in vivo migration of stem cell
a stem cell and in vivo technology, applied in the field of inducing in vivo migration of stem cells, can solve the problems of repeated surgery, impaired tissue regeneration, limited donors, etc., and achieve the effect of promoting/inducing cell homing, efficient utilization of cell resources, and convenient and efficient use of cell resources
Active Publication Date: 2015-12-29
TEGO SCI
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Benefits of technology
The present invention provides an implantable composition for treating damaged tissue by inducing the in vivo migration of cells to a damaged tissue region. The composition can be applied to treat bone, joint cartilage, or skin tissue, and can also be used as a treatment supplement agent in cell therapy using allogeneic cells, which are in high demand. This approach enhances the efficacy of cell-based therapy and allows for efficient utilization of cell resources.
Problems solved by technology
However, there are disadvantages that the surgery is repeatedly required and donors are limited.
However, there are disadvantages that the result is erratic and insufficient mesenchymal stem cells lead to impaired tissue regenerations [3-7].
Conventional treatments used for articular damages (for example, drug therapy, surgery, gene therapy, etc.) rarely give the desired result.
However, there are many difficulties in the actual transplantation due to limitations of donation parts or donors, high-cost, repeat surgeries and significantly impaired functions of the transplanted cells.
Although artificial substitutes composed of complexes in which biodegradable synthetic polymer are coated with cells has been recently attempted to clinical applications, they have not been able to overcome the current problems.
Likewise, various methods for regenerating the damaged articular cartilage tissues have been developed, but it is limited and insufficient to clinical applications.
Method used
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Materials and Methods
Cell Culture
[0074]Human bone marrow-derived mesenchymal stem cells (BMSCs) were aspirated from the posterior iliac crest of patients after approval by the Institutional Review Board (IRB), centrifuged to precipitate pellet. The resultant supernatant was isolated, wished and seeded with DMEM-LG (Dulbecco's modified eagle's medium-low glucose; GIBCO-BRL, Grand Island, N.Y., USA) containing 10% FBS (fetal bovine serum; GIBCO BRL, Grand Island, N.Y., USA), 100 units / ml of penicillin G (GIBCO) and 0.1 mg / ml of streptomycin (GIBCO) into T-75 cm2 cell flask (Falcon, Germany), followed by incubating for 4 days. Then, cells unattached to the bottom of the flask were removed. The MSCs attached to the bottom of the flask were exchanged with media every 3 days, and sub-cultured until nearly 90% confluent. BMSCs (passage 2-3) were used in all experiments.
In Vitro Mimicking of Articular Cartilage Injury
[0075]After serum starvation of BMSCs in serum-free DMEM-LG for 12 hours, ...
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The present invention relates to an implantable composition for treating a damaged tissue and a method for inducing an in vivo migration of a cell for treatment to a damaged tissue region. The present invention treats the damaged tissue by inducing / promoting homing of a cell for tissue generation by implanting a biodegradable scaffold reacted with chemotactic factors (for example, IL-8 or MIP-3α) to a damaged location (for example, joint cartilage or skin). Thus, the composition of the present invention can not only be applied to the treatment of a damaged bone tissue, a joint cartilage, or a skin tissue more conveniently and efficiently compared to the conventional technology, but can also be used as a useful treatment supplement agent in cell treatment using allogeneic cell by enabling efficient utilization of cell resources for treatment, the cell resources which are high in scarcity.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is the U.S. national stage filing under 35 U.S.C. §371 of international application PCT / KR2012 / 000618, filed Jan. 26, 2012, which claims priority from Korean Patent Applications 10-2011-0020221, filed Mar. 8, 2011 and 10-2012-0001563, filed Jan. 5, 2012.FIELD OF THE INVENTION[0002]The present invention relates to an implantable composition for treating a damaged tissue and a method for inducing an in vivo migration of a cell for treatment to a damaged tissue region.DESCRIPTION OF THE RELATED ART[0003]Stem cells are cells that can differentiate into various cell types constituting the tissues of an organism, and collectively refer to the cells in an undifferentiated state that can be obtained from embryonic, fetal and adult tissues. The stem cells differentiate into specific cell types under necessary stimulation (environment). Unlike the differentiated cells whose cell division has stopped, they can produce the cells iden...
Claims
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Patent Timeline
Login to View More IPC IPC(8): A61L27/54A61L27/56A61K38/20A61K38/19A61L27/18A61L27/58C07K17/02C07K14/54C07K14/52
CPCA61K38/2053A61K38/195A61L27/54A61L27/56A61L27/58C07K14/5421C07K17/02C07K14/523A61L2430/24A61L2300/252A61L2300/412A61P17/00A61P17/02A61P19/02A61P19/08A61P43/00A61K38/18A61K38/20A61K47/30A61K47/50
Inventor LEE, JIN WOOPARK, MIN SUNGKIM, YUN HEE
Owner TEGO SCI