4 results about "Membrane coat" patented technology

The invention discloses an MKP7 targeted bionic nanoparticle preparation as well as a preparation method and application thereof. The MKP7 targeted bionic nanoparticle preparation is a bionic nanoparticle siMKP7 (at) NP-M-pHLIP, wherein the bionic nanoparticle siMKP7 (at) NP-M-pHLIP is coated with a macrophage membrane modified by pHLIP and is loaded with siMKP7. According to the preparation, a PLGA-PEI copolymer is used as a carrier core to wrap siMKP7, an outer layer is coated with a macrophage membrane, the surface of the membrane is modified with pHLIP targeting peptide, and spherical nanoparticles with bionic camouflage and pH sensitive targeting delivery functions are constructed. In-vitro experiments show that the nanoparticles can be effectively ingested by macrophages, and macrophage lipid deposition induced by ox-LDL is remarkably inhibited; in-vivo experiments prove that the preparation can be delivered to atherosclerotic plaque parts in a targeted manner, and by inhibiting expression of MKP7 in macrophages, the carotid plaque area is effectively reduced, the serum inflammatory factor level is reduced, and the blood lipid metabolism is regulated, so that development of atherosclerosis is inhibited, and the curative effect of atherosclerosis is improved. A high-efficiency and low-toxicity nucleic acid drug delivery system is provided for treatment of atherosclerosis.

This invention discloses a multifunctional biomimetic nanoparticle, its preparation method, and its applications. The multifunctional biomimetic nanoparticle comprises: a PBAE / pDNA nanocomposite, a macrophage membrane coated on the PBAE / pDNA nanocomposite, and an IL-7R targeting peptide anchored on the macrophage membrane. The multifunctional biomimetic nanoparticle provided by this invention first synthesizes a biodegradable poly-β-amino ester polymer via a two-step method, then electrostatically combines it with ETS1 therapeutic plasmid DNA to form a PBAE / pDNA nanocore; subsequently, this nanocore is coated with a macrophage membrane to construct the biomimetic nanoparticle; finally, the IL-7R targeting peptide (ITP) is modified on the surface of the macrophage membrane using lipid fusion technology to obtain the final product PBAE / pDNA@ITP-MM.

The invention discloses a multifunctional bionic nano-particle as well as a preparation method and application thereof. The multifunctional bionic nano-particle comprises a PBAE / pDNA nano-composite, a macrophage membrane coated on the PBAE / pDNA nano-composite, and an IL-7R targeting peptide anchored on the macrophage membrane. The multifunctional bionic nano-particle provided by the invention is prepared by the following steps: firstly, synthesizing a biodegradable poly-beta-amino ester polymer by a two-step method, and then carrying out electrostatic compounding on the biodegradable poly-beta-amino ester polymer and ETS1 therapeutic plasmid DNA to form a PBAE / pDNA nano-core; then, the nano core is coated with a macrophage membrane, and bionic nano particles are constructed; finally, the surface of a macrophage membrane is modified with IL-7R targeting peptide (ITP) through a lipid fusion technology, and a final product PBAE / pDNA (at) ITP-MM is prepared.

This utility model belongs to the field of manure fermentation technology, specifically a membrane-coated accelerated fermentation manure fermentation tank, comprising: a fermentation tank body, with a W-shaped guide plate and a porous aeration pipe arranged on the bottom surface of the fermentation tank body, symmetrically installed sliding rods inside the fermentation tank body, symmetrically opened support grooves on the inner wall of the fermentation tank body, and a first drive motor symmetrically installed on one side of the fermentation tank body, the output end of the first drive motor being fixedly connected to a bidirectional threaded rod; through the cooperation of the first drive motor, the bidirectional threaded rod, the connecting frame and the storage frame, one end of the membrane is driven to move along the top of the fermentation tank body, and the first support rods and connecting rings on both sides are driven to move along the sliding rods, stretching the two sides of the membrane, so that the membrane can more smoothly cover the top of the fermentation tank body, thereby improving the membrane coating efficiency of the fermentation tank body.