52 results about "Biomimetic nanoparticles" patented technology

The invention belongs to the technical field of biological medicine, and discloses a bionic nano-particle suspension formulation based on orthoester compounds and a preparation method of the bionic nano-particle suspension formulation. The bionic nanoparticle suspension formulation comprises a liquid auxiliary material, a photosensitizer and bionic nanoparticles, wherein the photosensitizer and the bionic nanoparticles are dispersed in the liquid auxiliary material. According to the present invention, an orthoester compound represented by a formula I is adopted as a liquid auxiliary material, bionic nanoparticles and a photosensitizer are simultaneously introduced into OE to obtain the bionic nanoparticle suspension formulation having multiple antitumor mechanisms, the bionic nanoparticles are cancer cell membrane vesicle-coated nano-drugs, and the cancer cell membrane vesicle-coated nano-drugs can be prepared through a cancer cell membrane biomimetic technology, such that the cancer cell membrane vesicle-coated nano-drugs can be prepared into the bionic nanoparticle suspension formulation having multiple antitumor mechanisms; the homologous targeting function provided by the cancer cell membrane is utilized, and the active targeting property and cellular uptake of the nano-drug are remarkably improved. Formula I: # imgabs0 #

The invention provides a preparation method of dendritic mesoporous silicon bionic nanoparticles loaded with RNA (Ribonucleic Acid) molecules, which comprises the following steps: preparing dendritic mesoporous silicon nanoparticles DMSNs by adopting a double-phase layering method, dissolving a surfactant CTAC and a catalyst TEA in water to serve as a water phase solution, dissolving ethyl silicate TEOS in cyclohexane to serve as an oil phase, slowly dropwise adding the oil phase into the water phase, and reacting for 2-4 hours to obtain the dendritic mesoporous silicon bionic nanoparticles loaded with the RNA molecules. The DMSNs nanoparticles are obtained; amino groups are introduced to the surfaces of DMSNs through gamma-aminopropyltriethoxysilane APTES so as to enhance electrostatic attraction to nucleic acid, and after a surfactant template is removed, the DMSNs are mixed with RNA molecules in ribozyme-free water; meanwhile, a cytoplasmic membrane is harvested by using a hypotonic method, and the dendritic mesoporous silicon bionic nanoparticles loaded with the RNA molecules are prepared through ultrasonic treatment and polycarbonate film extrusion. The method is simple and convenient, and has a wide application prospect in RNA delivery.

The invention discloses a glucan modified bionic nanoparticle as well as a preparation method and application thereof. Preparing nanoparticles based on PLGA as an inner core; the tumor cell membrane is extracted from B16F10 melanoma cells and is used as an outer capsid; glucan is coupled with a hydrophobic long chain through reductive amination and is modified on the surface of a biomimetic nanoparticle cell membrane through a hydrophobic effect. The dextran nano delivery system is successfully constructed, cell membrane specific antigens and dextran are efficiently and jointly delivered to lymph nodes, tumor cell membranes provide epitopes for immune activation of nanoparticles, and beta-dextran provides molecular support for nanoparticle targeting immune cells. In-vivo and in-vitro activity experiments prove that the glycolipid modified bionic nanoparticles can effectively activate innate immunity through macrophages and NK cells, can promote dendritic cell antigen presentation, and initiate potent adaptive immune response. The bionic nanoparticles have huge development potential of novel tumor vaccines, and a new thought is provided for immunotherapy of cancers.

The invention relates to rapamycin-loaded bionic nanoparticles as well as a preparation method and application thereof. The bionic nanoparticle loaded with the rapamycin comprises RZ NPs loaded with the rapamycin and a tumor cell membrane, the tumor cell membrane is coated with the RZ NPs loaded with the rapamycin; the rapamycin is modified in pores of the RZ NPs; and the RZ NPs has a crystal structure of ZIF8. The preparation method of the rapamycin-loaded bionic nano-particles comprises the following steps: preparing RZ NPs loaded with rapamycin, and modifying the RZ NPs by using a tumor cell membrane to prepare the RZM NPs. The RZM NPs provided by the invention has the capability of external irradiation sensitization on tumors, and due to the fact that rapamycin has potential influence possibility on cell cycles, tumor cells can be stagnated in a stage which is more sensitive to external irradiation, so that the radiotherapy effect is improved.

The invention discloses a matrix metalloproteinase-responsive multifunctional bionic ferritin nano material as well as a preparation method and application thereof. The method comprises the following steps: modifying melittin on the surface of a ferritin nanoparticle through a genetic engineering method, connecting by using a linker containing a matrix metalloproteinase 9 (MMP-2 / 9) restriction enzyme cutting site, expressing the obtained recombinant ferritin (SEQ ID NO: 6) in escherichia coli, and self-assembling to form the enzyme response functionalized bionic nanoparticle. According to the method, melittin can be connected to ferritin nanoparticles in series, the toxicity of melittin is remarkably reduced, and the melittin responds to matrix metalloproteinase in a tumor microenvironment so as to kill tumor cells; meanwhile, intravenous administration of melittin can be realized, and the melittin injection has a good treatment effect on in-situ and metastatic tumors.

The invention discloses bionic nanoparticles, a composition, a preparation method and application thereof, and relates to the technical field of red blood cell-cancer cell hybrid membrane bionics. The bionic nanoparticles comprise lipidosome and a hybrid membrane coated on the surface of the lipidosome, drugs are coated in the lipidosome, and the drugs are astragaloside and curcumin; the molar ratio of the medicine to the liposome is 1: 15; the hybrid membrane is a hybrid membrane of an erythrocyte membrane and a cancer cell membrane. The erythrocyte-cancer cell hybrid membrane wraps the outer layer of the liposome phospholipid bilayer to construct the nano targeting drug delivery system, the modification of the hybrid cell membrane integrates the advantages of long circulation, immune escape, homologous targeting and the like, and a new treatment mode is developed for immunotherapy of malignant tumors with low response rate. The hybrid membrane can improve the targeting property of the drug, reduce side effects on patients and improve the tolerance of the patients. The medicine can be coated in an aqueous solution layer of the liposome, so that the water solubility of the medicine is improved, and meanwhile, the anti-tumor effect of the medicine can be enhanced.

The present invention discloses a multifunctional biomimetic ferritin nanomaterial responsive to matrix metalloproteinases and its preparation method and application. The present invention modifies melittin on the surface of ferritin nanoparticles by a genetic engineering method, connects with a linker containing a matrix metalloproteinase 9 (MMP‑2 / 9) cleavage site, and obtains recombinant ferritin (SEQ ID NO: 6) expressed in Escherichia coli and self-assembled to form enzyme-responsive functionalized biomimetic nanoparticles. The present invention utilizes this method to connect melittin in series on ferritin nanoparticles, significantly reducing the toxicity of melittin, and responding to matrix metalloproteinases in the tumor microenvironment to kill tumor cells; At the same time, intravenous administration of melittin can also be achieved, with good therapeutic effects on both in situ and metastatic tumors.

The invention discloses an antibacterial peptide modified bionic nanoparticle targeting delivery system as well as a construction method and application thereof. The antibacterial peptide modified bionic nanoparticle targeting delivery system comprises a recombinant antibacterial peptide LL-37, a macrophage membrane and an enzyme cascade reaction system, the recombinant antibacterial peptide LL-37 is expressed on the macrophage cell membrane to form a macrophage cell membrane modified by the recombinant antibacterial peptide LL-37; the enzyme cascade reaction system is wrapped by the macrophage membrane modified by the recombinant antibacterial peptide LL-37, so that the bionic nanoparticle targeted delivery system modified by the antibacterial peptide is formed; wherein a Toll-like receptor 2 and a Toll-like receptor 4 are highly expressed on the surface of a macrophage cell membrane; the enzyme cascade reaction system comprises a nano enzyme with catalase-like activity and a hydrogen peroxide-producing biological enzyme. The antibacterial peptide modified bionic nanoparticle targeting delivery system disclosed by the invention can be used for treating infectious bone defects such as periodontitis, peri-implantitis, infectious fracture, osteomyelitis and the like.

The invention discloses an MKP7 targeted bionic nanoparticle preparation as well as a preparation method and application thereof. The MKP7 targeted bionic nanoparticle preparation is a bionic nanoparticle siMKP7 (at) NP-M-pHLIP, wherein the bionic nanoparticle siMKP7 (at) NP-M-pHLIP is coated with a macrophage membrane modified by pHLIP and is loaded with siMKP7. According to the preparation, a PLGA-PEI copolymer is used as a carrier core to wrap siMKP7, an outer layer is coated with a macrophage membrane, the surface of the membrane is modified with pHLIP targeting peptide, and spherical nanoparticles with bionic camouflage and pH sensitive targeting delivery functions are constructed. In-vitro experiments show that the nanoparticles can be effectively ingested by macrophages, and macrophage lipid deposition induced by ox-LDL is remarkably inhibited; in-vivo experiments prove that the preparation can be delivered to atherosclerotic plaque parts in a targeted manner, and by inhibiting expression of MKP7 in macrophages, the carotid plaque area is effectively reduced, the serum inflammatory factor level is reduced, and the blood lipid metabolism is regulated, so that development of atherosclerosis is inhibited, and the curative effect of atherosclerosis is improved. A high-efficiency and low-toxicity nucleic acid drug delivery system is provided for treatment of atherosclerosis.

The application belongs to the technical field of biological medicine, and particularly relates to a nano delivery system based on exosome membrane fusion and a preparation method and application thereof, which comprises: (1) a prodrug complex formed by paclitaxel (PTXL) and IR780; (2) a liposome core loaded with the prodrug complex, and a membrane material of the liposome core comprises: DSPE-PEG-MAP, DSPE-PEI, phospholipid and cholesterol; and (3) a homologous tumor cell-derived exosome membrane fused to the surface of the liposome core. The application realizes the design of multi-level delivery in the same nanoparticle by means of the optimization of the properties of the liposome membrane composition, the fusion of the exosome and the liposome membrane, the mixed biomimetic nanoparticle particle size, the preparation of the PTXL targeted prodrug and the like, so as to improve the treatment effect at different levels.

The present invention relates to compositions and methods of pulmonary surfactant-biomimetic nanoparticles. Compositions and methods containing pulmonary surfactant (PS) biomimetic nanoparticles are disclosed. Specifically, the present disclosure relates to compositions containing nanoparticles having an average size of 200-400 nm, the nanoparticles comprising a plurality of pulmonary surfactant biomimetic molecules wherein the nanoparticles are negatively charged; and one or more cargo molecules encapsulated by the nanoparticles, wherein the molecular weight of the cargo molecules is 1200 Da or less.

The invention relates to a viFSP1 and sorafenib combined ferroptosis enhanced liver cancer treatment system based on bionic nanoparticles as well as preparation and application of the viFSP1 and sorafenib combined ferroptosis enhanced liver cancer treatment system, belongs to the field of electrochemical energy storage and new energy materials, and discloses a bionic nano delivery system for combined use of an FSP1 inhibitor viFSP1 and sorafenib. Ferroptosis is induced by targeting liver cancer cells and M2 type tumor-related macrophages so as to improve the treatment effect of sorafenib, and the immunosuppressive tumor microenvironment is effectively reversed, so that an efficient treatment method of liver cancer is realized.

The application discloses a kind of selectively targeted PSMA's biomimetic nanoparticles and its preparation method and application.This application simulates the glutamic acid substrate of carboxypeptidase and designs new functional monomer N-acrylamido glutaric acid and N-acrylamido succinic acid, a series of targeted PSMA's biomimetic nanoparticles with high affinity and selectivity to PSMA are synthesized using new monomer.The nanoparticle has low cost, higher stability, longer service life and stronger ability to resist harsh environment, overcomes the shortcomings of high cost, low preparation efficiency, long screening cycle, difficult to save and other defects of protein antibody.This application synthesizes water-soluble carbon dots with near-infrared fluorescence and photothermal properties, combined with the targeted PSMA's biomimetic nanoparticles, can replace protein antibody, nuclide imaging molecule and be used for targeted recognition of tumor cells and effective fluorescence imaging to early diagnose cancer, further kill tumor cells through photothermal therapy.

The invention belongs to the technical field of biological medicine, and relates to a vascular endothelium targeted bionic nanoparticle and a preparation method and application thereof.The preparation method of the bionic nanoparticle comprises the steps that 1, STM2457 is dissolved, NH2-MSN is added, the mixture is stirred in a dark place, and STMat MSN is obtained; (2) adding the STM (at) MSN and adamantane carboxylic acid into DMF (Dimethyl Formamide) to obtain ade-MSN; (3) adding the ade-MSN into a PBS (Phosphate Buffer Solution), adding beta-CD, and stirring, so as to obtain STM2457 (at) CD-ade-MSN; (4) mixing the liposome with the platelet membrane, and adding the product obtained in the step (3) to obtain STM2457 (at) CD-ade-MSN / PL; and (5) mixing the product in the step (4) with the reduced CD31 antibody to obtain the bionic nanoparticles. The vascular endothelium targeted bionic nanoparticles prepared by the invention can be used for preventing, relieving or / and treating cardiovascular toxicity caused by chemotherapeutic drugs.

The application provides a preparation method and application of a pH / ROS dual-responsive functionalized biomimetic nanomaterial loaded with mannose. The nanomaterial takes a pH / ROS dual-responsive material as a carrier and simultaneously loads a mannose drug. The prepared nanoparticles are wrapped with functionalized macrophage membranes on the surface of the nanoparticles by a lipid extruder to achieve targeted drug delivery. The functionalized biomimetic nanoparticles provided by the application can rapidly release mannose in an acidic and / or high active oxygen microenvironment, thereby effectively promoting the generation of cartilage cell extracellular matrix. The application provides a promising strategy for targeted treatment of intervertebral disc degeneration induced by cartilage injury.

The invention relates to the field of biological medicines and nano materials, in particular to a macrophage membrane-coated pH responsive bionic nanoparticle which can target tumors and is used for combination of photothermal therapy and immunotherapy as well as a preparation method and application of the macrophage membrane-coated pH responsive bionic nanoparticle. The bionic nano-drug delivery system disclosed by the invention can specifically target tumor cells and tissues, prolong the residence time of the drug in the bladder, improve the anti-tumor curative effect, enhance systemic immune activation, reduce adverse reactions and effectively inhibit tumor metastasis and relapse, and shows good clinical application potential.

A small activating RNA (saRNA) capable of activating a CCAAT enhancer binding protein alpha (CEBPA) gene, and a delivery system and use thereof are provided, where sense and antisense strands of the saRNA have nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 2, respectively. A biomimetic nano-delivery system for targeted delivery of the saRNA is a biomimetic nanoparticle formed by composite nanoparticle coated by a biomembrane of an inflammatory effector cell, where the composite nanoparticle is formed by histone loading with the saRNA capable of activating the CEBPA gene. The biomimetic nanoparticle can inherit antigens and related membrane functions of the inflammatory effector cell and specifically accumulate at the sites of inflammatory lesions, which enables the biomimetic nanoparticle to not only have a longer circulation time in vivo, but also show inflammatory tropism.

The invention discloses a phototherapy nano system for removing candida and lactobacillus, a preparation method of the phototherapy nano system and application of the phototherapy nano system in preparation of drugs for treating vulvovaginal candidiasis. The phototherapy nano system comprises cell membranes with GPIb, GPIIb / IIIa and gC1q-R / P32 receptors; polylactic acid-glycolic acid copolymer (PLGA) nanoparticles wrapped by a cell membrane; a photosensitizer is embedded on the surface of the cell membrane; the preparation method comprises the following steps: extracting cell membrane PM of human platelets; the method comprises the following steps: premixing an IR780 photosensitizer and a cell membrane PM, and embedding IR780 into the cell membrane PM through ultrasonic under a water bath condition to obtain PM (IR); the preparation method comprises the following steps: carrying out water bath ultrasonic treatment on PM (IR) and polylactic acid-glycolic acid copolymer PLGA nanoparticles, and coating the PM (IR) on the polylactic acid-glycolic acid copolymer PLGA nanoparticles to obtain the PM (IR)-NP bionic nanoparticles. The problems of high recurrence rate, drug tolerance and vaginal flora ecological imbalance in the treatment of the recurrent vulvovaginal candidiasis can be relieved.

The invention discloses a bionic nanoparticle based on cancer cell membrane modification and a preparation method and application thereof, belongs to the technical field of nano drug loading, and particularly relates to a bionic nanoparticle based on cancer cell membrane modification, which comprises a carrier, a utility agent loaded on the carrier and a cancer cell membrane coated on the outer layer, the carrier contains CeO2 and MoS2, the CeO2 is of a hollow structure, and the MoS2 is combined on the hollow structure of the CeO2. The bionic nanoparticles based on cancer cell membrane modification prepared by the invention are good in targeting, good in anti-tumor activity, low in cytotoxicity, good in tumor growth inhibition and tumor targeting accumulation capacity, long in circulation time and capable of effectively killing tumor cells.

The application discloses a method for treating premature ovarian insufficiency by using a double-targeting biomimetic nanoparticle based on a wolfberry-derived extracellular vesicle, relates to the technical field of premature ovarian insufficiency, and obtains wolfberry-derived extracellular vesicles, uses the wolfberry-derived extracellular vesicles as a nano delivery carrier, and realizes the treatment effect on the damage related to chemotherapy-induced premature ovarian insufficiency by using active ingredients carried by the wolfberry-derived extracellular vesicles; the wolfberry-derived extracellular vesicles are coated by using homologous ovarian granulosa cell membranes, and a GCM-LBEVs biomimetic nanoparticle vesicle with granulosa cell recognition ability is constructed. The application realizes the accurate delivery of ovarian granulosa cells and mitochondria by constructing a TPP-GCM-LBEVs double-targeting biomimetic nano system, improves the quality of oocytes, ovarian function and fertility, and provides a new nano strategy for the treatment of POI and mitochondrial-related reproductive disorders.

The present application relates to the technical field of nanocapsules for medical preparations, and particularly relates to dTRIM24 biocompatibility-based anti-atherosclerotic nanoparticles composed of nanoparticles and M1 macrophage membrane vesicles. By using a microfluidic photoporation chip, the nanoparticles are encapsulated by the M1 macrophage membrane vesicles. The nanoparticles are obtained by combining dTRIM24 and Fe3O4 magnetic nanoparticles. The biomimetic nanoparticles of the present application reasonably solve the problems of poor specificity and low efficiency, and have improved anti-atherosclerotic plaque-targeting and biological homology.

The invention discloses TIM-3 scFv bionic nanoparticles loaded with an A-D-A organic sound-sensitive agent and a preparation method and application of the TIM-3 scFv bionic nanoparticles loaded with the A-D-A organic sound-sensitive agent. The preparation method comprises the steps that a tetrahydrofuran solution of CH-H and a tetrahydrofuran solution of DSPE-PEG are added into deionized water, ultrasonic treatment is carried out, then rotary evaporation is carried out, concentration and drying are carried out, and the A-D-A organic sound-sensitive agent nanoparticles are obtained; the method comprises the following steps: obtaining a cell membrane of a macrophage with stable overexpression of an EGFP-TIM-3 scFv gene, then mixing the cell membrane with A-D-A organic sound-sensitive agent nanoparticles, and extruding through an extruder; the organic sound-sensitive agent can efficiently generate ROS and can directly kill tumor cells and induce ICD, meanwhile, TIM-3 scFv blocks TIM-3 signals on cDC1 cells, the antigen presentation function of the cDC1 cells is recovered, positive feedback circulation of sonodynamic killing, antigen release, cross presentation and T cell activation is formed, and the aim of treating colorectal cancer is achieved.

The present application relates to gossypol acetic acid, and particularly relates to a kind of targeted delivery gossypol acetic acid prodrug chemical programmable biomimetic nanoparticles and application thereof;The drug delivery carrier has the characteristics of non-toxicity, good biocompatibility and simple preparation, in addition, its particle size is small, and the targeting ability is strong, is conducive to direct targeting to liver stellate cell.In addition, the design of prodrug makes RAET NPs remain inert state under normal physiological conditions, to minimize systemic toxicity.And in the fibrosis microenvironment of high active oxygen level, prodrug is selectively converted into active form, to realize controllable release at lesion site.

The invention aims to provide a preparation method and application of bionic nanoparticles for treating rheumatoid arthritis, and belongs to the technical field of preparation of nano biomedical materials.According to the nanoparticles, hollow carbon spheres serve as carriers, platinum particles are loaded through an impregnation method, and through a bionic modification strategy of a macrophage membrane, the bionic nanoparticles are prepared. The invention constructs a bionic nano-particle for treating rheumatoid arthritis. The nanoparticles can generate singlet oxygen under the condition of no light stimulation, realize cascade generation of singlet oxygen by virtue of catalase-like activity and photo-thermal performance of the nanoparticles, and efficiently kill abnormally proliferated synovial fibroblasts at the rheumatoid arthritis part so as to treat rheumatoid arthritis.

The invention is applicable to the technical field of pharmaceutical preparations, and provides bionic nanoparticles MDI-CCM as well as a preparation method and application thereof. Bionic nanoparticles MDI-CCM are constructed, based on mesoporous silica nanoparticles loaded with doxorubicin (DOX) and indocyanine green (ICG), acid-sensitive controlled-release column [5] arene (WP [5] as a nano valve) is constructed on the surface, a tumor cell membrane is coated to realize homologous targeting of tumor cells, the nano valve triggers controllable release of drugs in a tumor microenvironment, and the drug delivery effect is improved. DOX chemotherapy and ICG near-infrared laser photo-thermal effect form synergy, and the treatment effect on 4T1 breast cancer tumors is enhanced. Experimental results show that the MDI-CCM can significantly inhibit tumor growth, has the characteristics of low toxicity, high drug loading capacity, active targeting and intelligent controlled release, can be applied to homologous targeting and chemotherapy-photothermal synergistic treatment of cancers such as breast cancer, and provides new insight and basis for related treatment.

The invention belongs to the field of medical biology, and relates to an STING agonist bionic nano delivery system and a preparation method and application thereof. The bionic nanoparticle containing the STING agonist comprises a shell, a drug carrier and an active component, the shell wraps the drug carrier, the drug carrier loads the active component, and the shell is a tumor cell membrane after ferroptosis induction; wherein the drug carrier is PLGA; wherein the active component is an STING agonist, and the STING agonist comprises a small molecule non-CDN (Content Delivery Network) type STING agonist; the bionic nano-particles are prepared by adopting a nano-precipitation method. The problem that an existing small molecule non-CDN STING agonist drug delivery system is poor in selectivity is solved.

The present invention relates to compositions and methods of pulmonary surfactant-biomimetic nanoparticles. Compositions and methods containing pulmonary surfactant (PS) biomimetic nanoparticles are disclosed. Specifically, the present disclosure relates to compositions containing nanoparticles having an average size of 200-400 nm, the nanoparticles comprising a plurality of pulmonary surfactant biomimetic molecules wherein the nanoparticles are negatively charged; and one or more cargo molecules encapsulated by the nanoparticles, wherein the molecular weight of the cargo molecules is 1200 Da or less.

This invention provides a biomimetic nanoparticle composite PRP hydrogel with anti-fibrotic and anti-inflammatory functions, its preparation method, and its application. The biomimetic nanoparticle composite PRP hydrogel is formed by cross-linking a hydrogel matrix and composite nanoparticles dispersed therein. The core of the composite nanoparticles is albumin nanoparticles loaded with macrophage migration inhibitory factor inhibitors, and the outer shell of the composite nanoparticles is a fibroblast membrane. The preparation method includes the following steps: preparing a composite nanoparticle dispersion; mixing sodium alginate with the composite nanoparticle dispersion to prepare a sodium alginate solution; adding platelet-rich plasma and mixing evenly; adding calcium carbonate and an acidity regulator; stirring evenly and allowing to stand to form a gel, thus obtaining the biomimetic nanoparticle composite PRP hydrogel with anti-fibrotic and anti-inflammatory functions.

The present invention discloses biomimetic nanoparticles modified with cancer cell membranes, their preparation methods, and applications. These nanoparticles, belonging to the field of nano-drug delivery technology, specifically relate to biomimetic nanoparticles modified with cancer cell membranes, comprising: a carrier, an agent loaded on the carrier, and an outer layer of a cancer cell membrane; the carrier contains CeO2 and MoS2, the CeO2 having a hollow structure, and the MoS2 bound to the hollow CeO2 structure. The biomimetic nanoparticles modified with cancer cell membranes prepared by the present invention exhibit excellent targeting, anti-tumor activity, low cytotoxicity, tumor growth inhibition, excellent tumor-targeting accumulation, long circulation time, and can effectively kill tumor cells.