The invention discloses a water quality monitoring device with a sampling function and a use method thereof. The water quality monitoring device comprises a bottom plate, a universal wheel is fixedly connected to the position, close to the left side, of the bottom of the bottom plate, two first rolling wheels are arranged at the position, close to the right side, of the bottom of the bottom plate, and supporting plates are arranged on the sides, away from each other, of the two first rolling wheels; the supporting plate is fixedly connected to the bottom of the bottom plate, a monitoring equipment body is arranged at the position, close to the left side, of the top of the bottom plate, supporting legs are fixedly connected to the bottom of the monitoring equipment body, the supporting legs are fixedly connected to the top of the bottom plate, and a fixing plate is arranged under the monitoring equipment body. An electric telescopic rod is fixedly connected to the right side of the fixing plate, a connecting plate is fixedly connected to the right end of the electric telescopic rod, a placing plate is fixedly connected to the bottom of the connecting plate, and a winding roller is arranged at the position, close to the left side, of the top of the placing plate.