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Refining method of ginkgo flavone

A technology of ginkgo flavonoids and ginkgo leaves, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc. It can solve the problems of high investment in supercritical extraction equipment, restrictions on production and application of flavonoids, and residual organic solvents in products, etc. problems, achieve the effects of saving organic and inorganic reagents, increasing the content, and shortening the production cycle

Inactive Publication Date: 2008-04-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the cost of solvent extraction is high, and the investment in supercritical extraction equipment is large, and it has not yet been industrialized.
The production of Ginkgo biloba extract (GBE) in China basically adopts the resin adsorption method, but this process also has the disadvantages of long operation time and high consumption of organic solvents
[0004] Due to the poor water solubility of flavonoids, the consumption of organic solvents such as ethanol is large, and organic solvents are easy to remain in products. The above problems have greatly restricted the production and application of flavonoids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 20g ginkgo biloba extract (GBE) was dissolved in 5000mL ethanol-water with ethanol mass fraction of 60%. The solution was subjected to the first ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. A polyvinylidene fluoride (PVDF) ultrafiltration membrane with a molecular weight cut-off of 10,000 dalton was selected (provided by Shanghai Xinya Purification Device Factory, the same below). Collect permeate. The collected permeate was evaporated to dryness, and then 1 part by mass of the evaporated powder was dissolved in 50 parts by mass of absolute ethanol. Gradually add methylated β-cyclodextrin, so that the ratio of flavonoids and methylated β-cyclodextrin in the solution is 1:3. First react in a 60°C water bath for 2h, and then react at room temperature for 4h. The reacted product was subjected to a second ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. A PVDF ultrafiltration membrane with a molecular weight cut-off of 5000dal...

Embodiment 2

[0019] 20g ginkgo biloba extract (GBE) was dissolved in 5000mL ethanol-water with ethanol mass fraction of 60%. The solution was subjected to the first ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. Choose a PVDF ultrafiltration membrane with a molecular weight cut-off of 10,000dalton. Collect permeate. The collected permeate was evaporated to dryness, and then the evaporated powder was dissolved in absolute ethanol. Add methylated β-cyclodextrin gradually, so that the ratio of flavonoids and methylated β-cyclodextrin in the solution is 1:9. First react in a 60°C water bath for 2h, and then react at room temperature for 4h. The reacted product was subjected to a second ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. A PVDF ultrafiltration membrane with a molecular weight cut-off of 5000dalton was selected. The concentrated liquid is collected to obtain the final product. It is determined that the percentage content of flavonoids...

Embodiment 3

[0021] 20g ginkgo biloba extract (GBE) was dissolved in 5000mL ethanol-water with ethanol mass fraction of 60%. The solution was subjected to the first ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. Choose a PVDF ultrafiltration membrane with a molecular weight cut-off of 10,000dalton. Collect permeate. The collected permeate was evaporated to dryness, and then the evaporated powder was dissolved in absolute ethanol. Add methylated β-cyclodextrin gradually, so that the ratio of flavonoids and methylated β-cyclodextrin in the solution is 1:6. First react in a 60°C water bath for 2h, and then react at room temperature for 4h. The reacted product was subjected to a second ultrafiltration at a pressure of 0.15 MPa and a temperature of 30°C. A PVDF ultrafiltration membrane with a molecular weight cut-off of 5000dalton was selected. The concentrated liquid is collected to obtain the final product. It is determined that the percentage content of flavonoids...

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Abstract

A refining method of ginkvone is carried out by dissolving GBE into 60% alcohol monohydrate in proportion of 1:200mass ratio, first super-filtering the GBE solution at 0.05-0.3Mpa and 10-50 DEG C, collecting filtrate, decompress concentrating the collected filtrate, dissolving residual steamed into anhydrous ethanol in proportion of 1:50 mass ratio, adding beta-cyclodextrin or modified beta-cyclodextrin into the solution obtained in proportion of flavone:beta-cyclodextrin or modified beta-cyclodextrin=1:9-1:1, reacting in water bath at 20-90 DEG C for 15mins-5hrs, reacting at normal temperature for 30mins-24hrs, second super-filtering the reactant at 0.05-0.3Mpa and 10-50 DEG C, and collecting the concentrated liquid to obtain the final product. It is simple, has short production period and more content and saves raw materials.

Description

technical field [0001] The invention relates to a method for extracting and preparing total flavonoids, in particular to a method for extracting total flavonoids from ginkgo leaves by combining cyclodextrin inclusion inclusion and two-step ultrafiltration. Background technique [0002] Ginkgo biloba (ginkgo biloba), also known as ginkgo, is a gymnosperm and one of the oldest tree species in the world, known as a "living fossil". Ginkgo is a special product of my country. my country's ginkgo germplasm resources are extremely rich. The national ginkgo leaf output is more than 20,000 tons (by dry weight), accounting for about 70% of the world's total. Ginkgo biloba contains bioactive components such as flavonoids and terpenoids. These ingredients have quite strong anti-oxidation effects, can remove excess free radicals in organisms, prevent lipid peroxidation in tissues, and improve the immunity of the human body. They are the natural medicines of choice for the treatment of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07H17/07C07H1/08
Inventor 张志炳徐志红李磊武法文谭淑娟杨奕
Owner NANJING UNIV
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