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Method for extracting insulin type growth factor-1 from fresh pilose antler

An insulin-like and growth factor technology, applied in the field of extracting active ingredients from velvet antler, can solve the problems of reducing the application value of velvet antler, waste of resources, loss of physiological functions, etc.

Inactive Publication Date: 2008-05-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the traditional processing of velvet antler, velvet antler has to go through several steps of cooking, frying, drying, etc. Long-term high-temperature treatment will denature the active polypeptides such as IGF-1 and lose its physiological functions, thus greatly reducing the application value of velvet antler. cause a lot of waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of extractant: Mix 0.2 mol / L sodium carbonate and 0.2 mol / L sodium bicarbonate solution to prepare sodium carbonate-sodium bicarbonate buffer solution with pH 9.8.

[0018] Take 100g of fresh velvet antler crushed at 4-10°C, add 1000g of extractant, stir well and carry out ultrasonic extraction for 30 minutes, the extraction temperature is 20°C, and the ultrasonic power is 200w; after the ultrasonic extraction is completed, centrifuge for 20 minutes (-4°C, 5000~10000r / min); add 500g extractant to the filter residue, repeat the above extraction steps; combine the supernatant obtained by two centrifugation, the IGF-1 extraction rate is 3200~3500ng IGF-1 / g fresh velvet antler. The ultrasonic extract is concentrated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 20 kDa, the loss rate of IGF-1 is less than 3%, and no IGF-1 can be detected in the filtrate. Transfer the ultrafiltration concentrate into a fat-removin...

Embodiment 2

[0020] Example 2 Use 0.5 mol / L ammonia water and 1 mol / L sulfuric acid to prepare an alkaline solution with a pH of 11 as an extractant. Subsequent process conditions are the same as in Example 1, the extraction rate of IGF-1 is 3000-3300ng IGF-1 / g fresh velvet antler, growth hormone (GH) in velvet velvet can also be extracted at the same time, and the GH extraction rate is 20-40ng GH / g fresh velvet velvet .

Embodiment 3

[0021] Example 3 The extraction agent and ultrasonic enhanced extraction process are the same as in Example 1, and the ultrafiltration membrane with a molecular weight cut-off of 4kDa is used to carry out ultrafiltration and concentration of the extract. IGF-1 cannot be detected in the filtrate, and the entire ultrafiltration concentration operation loses IGF-1 The rate is 1.7%, but the filtration rate is much lower than the ultrafiltration membrane of 20kDa.

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Abstract

This invention discloses an extraction method of insulin-like growth gene from fresh pilose antler, belonging to a technique of extracting active components from pilose antler. Using pH 9-12 buffering solution of sodium carbonate-sodium bicarbonate, odium bicarbonate-sodium hydroxide, or alkaline solution made from dilute ammonia water and dilute acid as extracting reagent, through ultra-sound intensified extraction, ultra-filtration concentration, removing fat, acidified decomposition, ion exchange layer separation, repeated ultra-filtration and frozen dry second time etc. steps, to extract IGF-1 etc. active multiple peptide from fresh pilose antler. With this technique of the invent the extraction rate of IGF-1 can reach as high as 3200-3500ng IGF-1 / g of pilose antler; but with de-ion water as extraction reagent, under the same technical condition the extraction rate of IGF-1 only is below 1000ng IGF-1 / g of pilose antler. The prepared products may be applied for medication, health-care supplement and other side.

Description

technical field [0001] The invention relates to a method for extracting insulin-like growth factor-1 from fresh velvet antler, which belongs to the technology of extracting active ingredients from velvet antler. Background technique [0002] Antler is the unossified young horns of sika deer or red deer. It is a traditional and precious Chinese medicine in my country. Its therapeutic effect has been recorded in ancient herbal medicines. Modern medicinal chemical analysis shows that deer antler contains a variety of physiologically active ingredients, mainly amino acids, polypeptides, unsaturated fatty acids, phospholipids, polyamines, polysaccharides and hypoxanthine, p-hydroxybenzaldehyde, estrogen, steroids and trace elements Wait. Among them, proteins and polypeptides account for more than 50% of velvet components. With the deepening of velvet research, in recent years, a variety of growth factors that have important functions on body growth, development and regulation ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/65C07K1/14
Inventor 王金宇李淑芬
Owner TIANJIN UNIV