Immununity and gene alaysis system by multiple mark continuous injections

Abstract

An immunity and gene analysis system of multilable continuous injection uses the multilabel system and the impulse exciting intermittent measuring method to make it possible for detecting multiclassified signal and associated indensity signal simultaneously and to ensure the high sensitivity and very wide linearity range. The said system realizes the parallel detection of multitarget for the same specimen, which breaks the limitation of each detecting target that to be detected separately in the existing technology. This system has the advantages of combining the detection targets flexibly at one's desire and can be used widely in the departments of clinical diagnosis, medicolegal identification, etc..

Description

technical field [0001] The invention relates to the field of multi-marker continuous injection immunization and gene analysis system. Background technique [0002] Immunological detection and gene analysis technology have been widely used in today's biomedical field, and have become necessary conventional technical means in many departments such as clinical diagnosis, scientific research, environmental and health testing, forensic identification, and play an irreplaceable role. role. A large amount of work involves parallel determination of multiple indicators on the same specimen. For example, in the clinical diagnosis of endocrine diseases, it is often necessary to perform multiple determinations on the levels of hormones and their metabolites secreted by the entire pituitary-gonadal axis and related glands; organ transplantation In typing, forensic individual identification and paternity testing, it is more necessary to analyze multiple sites of human histocompatibility ...

AI Technical Summary

Problems solved by technology

Secondly, because the system uses particles as solid phase carriers, the scattering of excitation light interferes more seriously with the detector, which further weakens the signal-to-noise ratio and forces the use of photomultiplier tubes with lower sensitivity. Tube, PMT), the signal is detected by photocurrent amplification, and the Single Photon Counter (Single Photon Counter) with higher sensitivity and wider linear range cannot be used

Third, since the sample detection tube of the flow cytometer only allows a single cell to pass through, only particles with a diameter of about 4 microns (μM) can be used when selecting carriers, which creates a big bottleneck: on the one hand, for Improving the efficiency of immunization and nucleic acid binding must require the addition of a large number of particles (about millions per sample) to increase the surface area; on the other hand, due to the limitations of the detection tube diameter, liquid flow rate and detector sensitivity, even the most advanced flow cytometry The cytometer can only detect up to 2,000 particles per second, so it is impossible to detect all the particles. In practical applications, each item can only detect about 50 particles, which only accounts for a small part of the reaction carriers; Such small particles are difficult to achieve good uniformity in size, shape, fluorescence intensity, etc., which greatly increases the experimental error and reduces the sensitivity. Therefore, in the actual application of the instrument, even if the processed particles are processed Expensive pre-screening, also often misclassified

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