Remedy for corneal failure

A technology of cornea and corneal sensitivity, applied in sensory diseases, nervous system diseases, peptide/protein components, etc., can solve problems such as not found

Inactive Publication Date: 2008-08-27
SENJU PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0054] However, no reports were found that teach the presence of somatostatin receptors in the trigeminal nerve (corneal nerve) or the promotion of axon extension of the trigeminal nerve (corneal nerve) by somatostatin

Method used

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  • Remedy for corneal failure
  • Remedy for corneal failure
  • Remedy for corneal failure

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0124] Experimental Example 1 Expression of Somatostatin Receptor in Rabbit Trigeminal Nerve

[0125] 1) Animals used

[0126] Japanese white rabbits (body weight 2.0 kg) purchased from Fukusaki Rabbit Warren were used.

[0127] 2) Test method

[0128] Celactal (xylazine): ketamine injection (ketamine hydrochloride) = 0.5: 1 intramuscular injection (0.9 mL / kg) was given to Japanese white rabbits for general anesthesia. After cardiac perfusion with saline, the retina and trigeminal nerve were harvested separately. RNA was extracted from each tissue by the AGPC method using TRIzol reagent (manufactured by GIBCO BRL), genomic DNA was removed by DNase treatment, and a reverse transcription reaction was performed using SuperScript II (manufactured by GIBCO BRL). The cDNA was amplified under the reaction conditions described in Table 1 using Platinum Taq DNA polymerase (GIBCO BRL). As primers, the rabbit somatostatin receptor SSTR2 gene-specific primer (hereinafter referred to a...

experiment example 2

[0134]Axon extension promotion in cultured rabbit trigeminal nerve cells (in vitro experiment)

[0135] 1) Animals used

[0136] Japanese white rabbits (2-3 days old) purchased from Fukusaki Rabbit Warren were used.

[0137] 2) Test substance

[0138] As test substances, somatostatin (manufactured by CALBIOCHEM, Lot B33795) and NGF (NGF-7S, manufactured by Sigma) were used. Test substances were dissolved in phosphate buffered saline (PBS) to 100 μM somatostatin, 20 μg / mL NGF-7S. Prepared reagents were stored at -80°C and dissolved before use.

[0139] 3) Test method

[0140] Trigeminal neurons were isolated as reported by Chan et al. (Kuan Y. Chan and Richard H. Haschke. Exp. Eye Res. 41:687-699, 1985). More specifically, after the rabbit heart was perfused with saline under ether anesthesia, the trigeminal ganglion was excised, and the trigeminal ganglion was dispersed using a nerve dispersion solution (SUMITOMOBAKELITE Co., Ltd.), and the cells were plated on polymer-co...

experiment example 3

[0152] Functional changes in corneal sensitivity after corneal nerve dissection in rabbits (in vitro assay)

[0153] 1) Animals used

[0154] Male Japanese white rabbits (body weight 1.5kg-2.0kg) purchased from Fukusaki Rabbit Warren were used.

[0155] 2) Test substance

[0156] As test substances, somatostatin (manufactured by CALBIOCHEM, Lot B33795) and NGF (NGF-7S, manufactured by Sigma) were used. The test substance was dissolved in the vehicle shown below and used for the test.

[0157] Carrier formulation

[0158] Sodium chloride 0.9g

[0159] Sodium dihydrogen phosphate dihydrate 0.1g

[0160] Appropriate amount of sodium hydroxide

[0161] (pH 7.0)

[0162] Appropriate amount of distilled water for injection

[0163]

[0164] Total 100mL

[0165] 3) Test method

[0166] Celactal (xylazine): ketamine injection (ketamine hydrochloride) = 0.5: 1 intramuscular injection (0.9 mL / kg) was given to the r...

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Abstract

It is intended to provide a drug of a novel type for restoring the corneal sense after keratotomy or improving the dry eye symptom. Application of a somatostatin receptor agonist is useful in improving corneal dysesthesia following cataract operation, following LASIK operation, or associating corneal nerve degeneration such as neuroparalytic keratitis, corneal ulcer or diabetic keratopathy, and the dry eye symptom.

Description

technical field [0001] The present invention relates to a corneal nerve axon extension promoter comprising a somatostatin receptor antagonist, and a corneal nerve axon-based extension for restoring or improving corneal sensitivity or treating dry eye or corneal Agents for epithelial defects. Background technique [0002] Since the corneal nerve is severed by corneal surgical procedures such as laser photorefractive keratotomy (PRK), laser-assisted in situ keratectomy (LASIK), keratoplasty, etc., corneal sensitivity is said to usually decrease for about 3 weeks to 1 year. As a result of reduced function of corneal sensitivity, patients blink less frequently after corneal surgery, problematically exhibiting symptoms of dry eye. In dry eye patients, lacrimal hypofunction produces corneal hyposensitivity, which, in combination with additional lacrimal hypofunction, may exacerbate corneal surface disorders. However, currently restoration of corneal sensitivity after corneal sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P25/00A61P27/02A61P43/00A61K38/22A61K38/31
Inventor 高山美子中村义邦井上淳东光佳
Owner SENJU PHARMA CO LTD
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