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Method for extracting effective component of bamboo leaf and petiole

A technology of active ingredients and extraction methods, applied in the field of extraction of active ingredients of plants, can solve the problems of large energy consumption and few enzymatic hydrolysis techniques

Inactive Publication Date: 2009-01-07
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing methods for extracting active ingredients in bamboo leaves and bamboo petioles seldom use enzymatic hydrolysis technology. In order to ensure a higher extraction rate, a large amount of water is required to extract active ingredients such as polysaccharides, flavonoids, and proteins, and a large amount of water is required. energy to remove large amounts of water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select 1,500 kg of bamboo leaves and bamboo petioles. The bamboo leaves and bamboo petioles are subjected to conventional pretreatment, cleaned to remove impurities, branches, stems, and air-dried. Fully mash the leaves and stems in a mashing machine, pass through a 10-mesh sieve, and add water after the pretreatment Extract, filter, obtain filtrate a and filter residue b; Filtrate a and filter residue b further extract each active ingredient, it is characterized in that: the solid-liquid ratio between the pretreated bamboo leaf bamboo petiole and the amount of water added is: 1: 25; The extraction process adopts the addition of 0.5% cellulase to carry out enzymatic hydrolysis, the conditions of fiber enzymatic hydrolysis: pH is 4.7, temperature is 40 degrees centigrade, time is 2 hours.

[0025] The filtrate a undergoes a filtration step, specifically ultrafiltration first. The ultrafiltration adopts an ultrafiltration membrane with a molecular weight cut-off of 10,000....

Embodiment 2

[0029] A proteolysis step is also added to the water extraction process, and the proteolysis conditions are as follows: the amount of protease is 2%, the pH is 4.5, the temperature is 45 degrees Celsius, and the time is 1 hour. All the other are with embodiment 1.

[0030] After testing, the extraction rate of polysaccharides reaches 5.3%. The extraction rate of flavonoids is 6.2%. The yield of flavonoids in the proteolysis step is increased, and the extraction rate of polysaccharides is improved.

Embodiment 3

[0032] After the pretreatment, no enzyme is added, and water is directly added to the bamboo petiole of the bamboo leaf for water extraction. The extraction temperature of the water extraction is 60° C., the extraction solid-liquid ratio between the pretreated bamboo leaf and bamboo petiole and the amount of water added is 1:100, and after extraction for 5.5 hours, the pH value is controlled at 6.5. Filtrate a was filtered, heated and concentrated for 10 hours to remove most of the water, and then entered into other steps, and all the others were the same as in Example 1.

[0033] In order to obtain a flavonoid yield of 3.481%, a large amount of water must be used to dissolve and leach enough flavonoids, and the extraction solid-liquid ratio is as high as 1:100. However, in order to remove a large amount of water, it takes a long time to heat and evaporate, which consumes a lot of energy.

[0034]After the pretreatment, no enzyme is added, and water is directly added to the b...

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Abstract

The present invention relates to the extraction of effective components in plant material, and is especially the extraction process of effective components in bamboo leaf and bamboo leaf stem. Bamboo leaf and bamboo leaf stem after pre-treatment are extracted with water in 8-70 times and filtered to obtain filtrate and filter cake, and the filtrate and the filter cake are further extracted separately to obtain the effective components. The present invention features that during water extraction, cellulase as enzymolysing agent is added to reduce water consumption and power consumption greatly. The present invention has also the advantages of high extraction efficiency, high product yield, high product purity and no environmental pollution.

Description

technical field [0001] The invention relates to a method for extracting effective components of plants, in particular to a method for extracting effective components in bamboo leaves and bamboo petioles. Background technique [0002] Bamboo is a perennial evergreen plant of Poaceae (Poaceae) Bambooideae (Bambusoideae). There are about 60 genera and more than 1,200 species in the world. They are mainly distributed in Asia. China is known as the "Kingdom of Bamboo". All account for about 1 / 3 of the world. There are about 70 or 80 kinds of economic bamboo species cultivated in my country. Among them, moso bamboo is an excellent bamboo species in my country with the highest economic value. At present, my country's bamboo forest area has reached more than 4.4 million hectares. Widely used in light industry, food and other industries. [0003] According to Chinese literature, bamboo leaves are light in nature, slightly astringent, cold, sweet and bitter in taste, and have the funct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P17/06
Inventor 高荫榆游海陈芩阮榕生陈才水杨柏云陈文伟何小立
Owner NANCHANG UNIV