Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing biological flocculant using Proteus mirabilis

A technology of Proteus mirabilis and biological flocculant, which is applied in the field of preparation of biological flocculant to achieve the effect of reducing cost, shortening start-up time and low cost

Inactive Publication Date: 2009-08-26
TONGJI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports on the preparation of bioflocculants by Proteus mirabilis at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing biological flocculant using Proteus mirabilis
  • Method for preparing biological flocculant using Proteus mirabilis
  • Method for preparing biological flocculant using Proteus mirabilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This implementation mode is carried out according to the following steps:

[0025] A. Inclined preservation and transfer of strains

[0026] The composition (g / L) of the slant culture medium for the preserved strains is: beef extract 5, agar 20, peptone 12, NaCl 6, pH 7.2, sterilized (121° C., 30 min). In an aseptic operating environment, the strains of Proteus mirabilis were placed on the slant medium, cultured in a constant temperature incubator at 30°C for 2 days, stored in a refrigerator at 6°C, and used within 1 day.

[0027] B. Preparation of seed solution

[0028] In an aseptic operating environment, the strains on the slant medium were transferred to the seed medium (universal fermentation medium). The composition of the seed medium (g / L): glucose 22, urea 0.6, yeast extract 0.6, K 2 HPO 4 6. KH 2 PO 4 3, NaCl 0.2, (NH 4 ) 2 SO 4 0.3, MgSO 4 ·7H 2 O 0.3, pH 7.2, sterilized (121°C, 30min). The inoculated seed medium was cultured in a shaker incubator...

Embodiment 2

[0040] A. Inclined preservation and transfer of strains

[0041] Composition (g / L) of the slant culture medium for preserved strains: beef extract 3, agar 15, peptone 8, NaCl 4, pH 7.0, sterilized (115° C., 20 min). In an aseptic operating environment, the strains of Proteus mirabilis were placed on the slant medium, cultured in a constant temperature incubator at 25°C for 1 day, stored in a refrigerator at 2°C, and used within 1 day.

[0042] B. Preparation of seed solution

[0043] In an aseptic operating environment, the strains on the slant medium were transferred to the seed medium (universal fermentation medium). Composition of seed medium (g / L): glucose 18, urea 0.4, yeast extract 0.4, K 2 HPO 4 4. KH 2 PO 4 1, NaCl0.05, (NH 4 ) 2 SO 4 0.1, MgSO 4 ·7H 2 O 0.1, pH 6.8, sterilized (115°C, 20min). The inoculated seed medium was cultured in a shaker incubator at a temperature of 20°C and a shaker speed of 150r / min for 2 days, stored in a refrigerator at 2°C, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Utilize Proteus mirabilis to prepare the method for biological flocculant, relate to a kind of preparation method of biological flocculant, its process is as follows: A, Proteus mirabilis (Proteus mirabilis) is transferred to the slant culture medium, and preserved in refrigerator 2~6 ℃; B. Inoculate the preserved strains into the seed medium, and then culture them in a shaker incubator at 25-30°C to prepare the seed liquid; C. Inoculate the obtained seed liquid into cheap Put it in the production medium, then put it into the shaker, 25~30℃, 120~140r / min, 1~2 days to get the production medium fermentation liquid of the strain, and pass the fermentation liquid through a 4000~6000r / min centrifuge centrifuge Separation for 20-40 minutes, remove the precipitate, the supernatant is the liquid bio-flocculant, measure the flocculation rate; D, extract and purify the liquid bio-flocculant to obtain pure bio-flocculant. The invention has the advantages of low cultivation cost, short production cycle, high flocculation efficiency of 92.88-93.13% as measured, no secondary pollution after use, adaptability to large-scale production and industrial application, and has good popularization and application value.

Description

technical field [0001] The invention relates to a preparation method of a biological flocculant, in particular to a method for preparing a biological flocculant by using Proteus mirabilis. Background technique [0002] Flocculants are substances that can coagulate and precipitate suspended particles that are not easy to settle in liquids. They are widely used in industrial water treatment, domestic sewage treatment, food production and fermentation industries. Flocculants can generally be divided into three categories: ①Inorganic flocculants, such as aluminum sulfate, polyaluminum chloride, ferrous sulfate and alum; ②Synthetic organic polymer flocculants, such as polyethylene oxide, polystyrene sulfonate , polyacrylamide derivatives and polyethyleneimine, etc.; ③ natural organic polymer flocculants, such as cellulose, starch, polyglucosamine, sodium alginate and biological flocculants. Many inorganic flocculants are widely used due to their good flocculation effect and low ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/52C12N1/20C12R1/37
Inventor 夏四清王学江张志强杨阿明
Owner TONGJI UNIV