Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method

Abstract

This invention provides to a method for culturing degradation bacteria of long-residual herbicide clomazone. The method comprises: uniformly mixing soil samples with long-term application of long-residual herbicide clomazone, weighing 10 g, adding into 200 mg / L clomazone-rich culture solution 50 Ml, culturing in a shaking table of 120 rpm at 30 deg.C for 2 months, adding fresh culture solution 10 mL per week, adding clomazone concentration for each time (200, 400, 800, 1600, 2000, 3000, 4000 and 5000 mg / L, respectively), culturing for 1 week after the clomazone concentration in the culture solution is up to 5000 mg / L, coating on the inorganic salt culture medium containing 200 mg / L clomazone, picking up single colony, streaking for purifying, and storing purified bacteria. The bacteria can degrade long-residual herbicide clomazone, thus can be used for biological repairing of polluted soil.

Description

(1) Technical field [0001] The present invention relates to a biological preparation, the invention also relates to a method for preparing the biological preparation, and the present invention also relates to an application method of the biological preparation. (2) Background technology [0002] The use of chemical pesticides has greatly promoted the development of agricultural modernization, restored a large number of losses caused by weed damage and other reasons for agricultural production, and played a vital role in promoting the modernization of agricultural production. However, pesticides are foreign substances to the environment. After they enter the ecosystem, they must have an impact on the ecosystem. [0003] Herbicides with high activity and long residual effect, such as atrazine, chlorsulfuron-methyl, chlorimuron-methyl, imazethapyr, ethametsulfuron, clomazone, etc., have been widely used in Northeast my country in recent years. The increase in production and inc...

AI Technical Summary

Problems solved by technology

At present, only glyphosate-resistant soybeans, cotton, corn, wheat, rapeseed, and sugar beets are commercialized, and there are still great controversies about genetically modified products and foods; and it requires a lot of research funds and manpower, and it takes a long time The research process is still far away from the practical application

③The use of safeners involves the release of another chemical substance into the environment, which will cause secondary pollution

④ Use endogenous plant growth regulators (gibberellin, indole acetic acid, brassinolide and Kangkai, etc.) to alleviate the phytotoxicity of herbicides, but cannot purify the soil ⑤ Utilize microbial degradation from soil, It is an effective way to eliminate the pollution of long-residual herbicides

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