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Non-filling type nucleic acid extraction device and its preparing method and application

A non-filled, nucleic acid technology, which is applied to non-filled nucleic acid extraction devices and its application in nucleic acid extraction, can solve the problems of high processing costs and difficult to make plastic substrates.

Active Publication Date: 2010-06-09
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of chip has good repeatability and stable performance, but the disadvantage is that the processing cost is expensive
Since the PECVD silicon oxide layer needs to be made at high temperature (generally greater than 350 degrees Celsius), this method can only be made on high melting point materials such as silicon, and it is difficult to make it on plastic substrates
So far, there are no reports or applications of non-filled nucleic acid extraction chips based on plastic

Method used

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  • Non-filling type nucleic acid extraction device and its preparing method and application
  • Non-filling type nucleic acid extraction device and its preparing method and application
  • Non-filling type nucleic acid extraction device and its preparing method and application

Examples

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Effect test

Embodiment 1

[0047] Example 1. Preparation of sandwich-type non-filled nucleic acid extraction device and detection of its nucleic acid extraction effect

[0048] 1. Preparation of sandwich-type non-filled nucleic acid extraction device

[0049] In this embodiment, the commonly used plastic material PMMA (polymethyl methacrylate) is used as a base material to prepare a sandwich-type non-filled nucleic acid extraction device. The sandwich-type non-filled nucleic acid extraction device consists of two plastic substrate layers (one layer is cover body, one layer is the bottom plate) and an intermediate layer in the middle, the three-dimensional view of its decomposed structure is shown in figure 2 , its preparation method comprises the following steps:

[0050] 1) First process the plastic substrate layer and the middle layer into such figure 2 The shape and size shown: The length of the plastic substrate layer is 75mm, the width is 25mm, and the thickness is 1mm. One of them is used as t...

Embodiment 2

[0061] Example 2. Preparation of Pipeline Non-Filled Nucleic Acid Extraction Device and Detection of Its Nucleic Acid Extraction Effect

[0062] 1. Preparation of pipeline-type non-filled nucleic acid extraction device

[0063] 1. Micromachining of pipes

[0064] Compared with other ways of processing pipelines with polymer materials (hot pressing, pouring, injection molding, LIGA, etc.), micromachining is a faster and cheaper way to process microstructures with polymer materials. In addition, since there is no need to make a mold, the produced graphics do not need to be limited by the mold graphics, so this method has the advantage of being flexible and easy to use, and the drawings can be modified at any time to produce different graphics. prepared by micromachining Image 6 The shown pipeline-type non-filled nucleic acid extraction device (also known as pipeline-type PMMA nucleic acid extraction chip) of the present invention has a size of 104mm×70mm. The production meth...

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Abstract

The invention discloses a preparing method of non-filling type nucleic acid extracting device and application, which comprises the following steps: providing a plastic base material to produce the product; composing with at least one construction unit; setting the construction unit include a cap body of plastic material and a model adsorptive support; setting pipe and beard or cavity on the modeladsorptive support; applying closely with cap body; setting silicon oxide gel layer on the inwall of pipe and beard or cavity; setting at least one sampling hole and one out-sampling hole on the cap body or model adsorptive support corresponding position of pipe and beard or cavity. This invention possesses simple preparing method and cheap cost, which possesses wide using prospect.

Description

technical field [0001] The invention relates to a non-filled nucleic acid extraction device in the field of microfluidic chips and its preparation method and application, in particular to a non-filled nucleic acid extraction device made of plastic as a base material and its application in nucleic acid extraction. Background technique [0002] In recent years, biochip technology for nucleic acid analysis has developed rapidly. Currently, most of the reported nucleic acid analysis chips are used to analyze pure nucleic acid samples, which are extracted and purified by conventional laboratory techniques. Therefore, if you want to miniaturize the routine nucleic acid extraction steps in the laboratory, it is very important to construct a nucleic acid extraction chip, which will help to build a miniature full analysis that integrates sample extraction, biochemical reactions, and result detection in a true sense. system. [0003] Nucleic acid extraction refers to the process of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12Q1/68C07H21/00
Inventor 刘惯超邢婉丽郭旻
Owner CAPITALBIO CORP
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