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Milk product with specific immunity of anti-enterobacter sakazakii and the preparing method thereof

The technology of Enterobacter sakazakii and Enterobacter sakata is applied in the field of immune milk products containing IgY specific for Enterobacter sakazakii and its preparation, which can solve the problems of remaining Enterobacter sakazakii and other problems, and achieve stable product quality and performance. Effect

Active Publication Date: 2007-09-26
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the above-mentioned defects of existing milk products, the purpose of the present invention is to solve the problem of remaining pathogenic bacteria such as Enterobacter sakakigi in existing milk products, and provide a method that can effectively eliminate Enterobacter sakagi or simultaneously effectively eliminate Enterobacter sakagi New immune milk products for infants and young children against Enterobacter, Salmonella, Escherichia coli and infantile rotavirus attacks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] 1. Preparation of specific immune milk powder against Enterobacter sakafikita

[0032] 1. Production of anti-Enterobacter sakafi specific IgY or anti-Enterobacter sakafi specific composite IgY

[0033] 1.1. According to the residual bacteria detected in ordinary infant milk powder by the inspection and quarantine department in recent years and the pathogens obtained from infected children, the four common types of Enterobacter sakagii and Salmonella were screened and determined as representative pathogenic bacteria. Escherichia coli and infantile rotavirus are the main pathogens of infantile diarrhea.

[0034] 1.2. Respectively cultivate Enterobacter sakakiki and Salmonella, Escherichia coli and infant rotavirus

[0035] 1.2.1. Enterobacter sakagii:

[0036] Purchase four representative species of Enterobacter sakagii from the American Virus Center (ATCC), numbered as follows:

[0037] I.No.BAA-894: A species proposed in 2001 from the human body infected with Enterob...

Embodiment 1

[0128] Example 1--preparation of anti-Enterobacter sakafiki specificity chicken IgY by pure water extraction method

[0129] 1.1. Purchase four representative Enterobacter sakagii from the American Virus Center (ATCC), the numbers are as follows:

[0130] I.No.BAA-894: A species proposed in 2001 from a human infected with Enterobacter sakagii by eating milk powder in the United States

[0131] II.No.29004: Enterobacter cloacae parasitic in the cloaca (Enterobacter cloacae) [this species was obtained and cultivated by the US CDC from clinical children]

[0132]III.No.29544: Enterobacter flavus parasitic in children [this species was raised and cultivated from clinical children's larynx]

[0133] IV.No.51329: Standard strain of Enterobacter sakagii;

[0134] 1.2. Then cultivated by the following method respectively; then purified by sucrose density gradient ultracentrifugation or gel column chromatography to obtain purified four representative Enterobacter sakagii respectively...

Embodiment 2

[0151] Example 2--preparation of anti-Enterobacter sakafikita specific composite IgY by pure water extraction

[0152] 2.1. Purchase the most representative Enterobacter sakagii from the American Virus Center (ATCC), number: No.BAA-894;

[0153] 2.2. Then use the following methods to cultivate:

[0154] Medium: ATCC#3 Vegetable Soup: Nutritious Vegetable Soup (BD234000)

[0155] ATCC#3 Agar: Nutrient Agar (BD213000)

[0156] Environment: Temperature: 30°C

[0157] Atmospheric environment: oxygenated

[0158] Cultivation steps:

[0159] [1] Open the vial according to the attached instructions

[0160] [2] Use a #3 vegetable soup (5-6mL), suck 0.5-1.0mL with a dropper or 1mL with a pipette, and drop it into a small tube to soak the whole bacterial ball.

[0161] [3] Aseptically draw the same amount back into the vegetable broth. Shake well.

[0162] [4] Take a few drops from the vegetable broth and seed into another vegetable broth or #3 tilted agar or / and agar...

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Abstract

The invention relates to a method for preparing enterobacteria specific immunity milk product which contains enterobacteria specific IgY antibody. The preparation comprises that first cultivating single enterobacteria or various viruses, preparing single or composite antigen, then using the antigen to process high-immunity ejection into egg-laying bird, checking the immunity egg, preparing enterobacteria specific IgY or enterobacteria specific composite IgY, purifying, filtering into the final product, mixing the product with some milk powder, to obtain immunity milk product. The invention can effectively resolve the problem of infant milk powder that polluted by enterobacteria and salmonella.

Description

technical field [0001] The invention relates to a novel immune milk product, in particular to an immune milk product containing anti-Enterobacter sakafiki specific IgY and a preparation method thereof. Background technique [0002] On November 1, 2002, the US Food and Drug Administration (FDA) detected Enterobacter sakazakii from infant formula produced by Wyeth and recalled some of the company's products from the market. Subsequently, inspection agencies in other countries also successively detected pathogenic bacteria such as Enterobacter sakagii in infant milk powder produced by Mead Johnson and other large milk powder factories. [0003] This is a warning to humans: infant formula milk powder is not a sterile product and may contain pathogenic bacteria, such as Enterobacter sakagi and Salmonella. [0004] The World Health Organization (WHO) pointed out that in 2004, two underweight babies in France died in hospital after drinking infant formula contaminated with bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/152A61K35/20
CPCY02A50/30
Inventor 包晟王长安蔡婷英
Owner SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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