Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing chitin oligosaccharide or chitosan oligosaccharide by using immobilized enzyme technology

A technology of immobilizing enzymes and chitooligosaccharides, applied in the field of chemical biology of renewable resources, can solve the problems of non-reusable, harsh reaction conditions, poor stability, etc., and achieve high affinity, good mechanical properties, and stable chemical properties.

Inactive Publication Date: 2007-09-26
WUHAN UNIV
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since enzymes are composed of proteins, their high-level structures are very sensitive to their environment, have poor stability, harsh reaction conditions, and cannot be reused.
In addition, the hydrolyzate contains a certain complex of sugar and enzyme protein, which can cause pyrogen reaction, so it is not suitable to be used in food, medicine and other fields

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: first the FeSO of 0.5mol / L 4 ·7H 2 O and 0.5mol / L FeCl 3 ·6H 2 O was mixed at a molar ratio of 1:2, 5 mol / L NaOH was added, and vigorously stirred until an oily magnetic fluid suspension was obtained. Dissolving chitosan in an acetic acid solution with a volume percentage concentration of 1-5% is made into a chitosan solution with a weight-to-volume ratio of 3%. In 50mL of the chitosan solution, add 3mL of the magnetic fluid and stir evenly. Add 50mL of liquid paraffin, drop in the emulsifier span80, stir at high speed to make the system into an emulsion, add glutaraldehyde with a volume of 1% of the system volume as a crosslinking agent, and pour the emulsion that has fully reacted at 35-45°C into the other In 5 times the volume of NaOH / ethanol (1:1) mixture, keep stirring for a period of time, discard the oil layer and water layer, wash off the adsorbate on the particle surface, and obtain a black magnetic shell with a particle size of 20-30um and a r...

Embodiment 2

[0014] Embodiment 2: first the FeSO of 0.25mol / L 4 ·7H 2 O and 0.25mol / L FeCl 3 ·6H 2 O was mixed at a molar ratio of 1:2, 5 mol / L NaOH was added, and vigorously stirred until an oily magnetic fluid suspension was obtained. Dissolve chitosan in an acetic acid solution with a concentration of 1-5% by volume to form a chitosan solution with a weight-volume ratio of 4%. Add 10mL of magnetic fluid to 50mL of the chitosan solution, stir evenly, and add 50mL of liquid paraffin, drop emulsifier span80, stir at high speed to make the system into an emulsion, add a volume ratio of 3% glutaraldehyde as a cross-linking agent, and pour the fully reacted emulsion at 35-45°C into 5 times the volume of NaOH / ethanol (1:1) mixed solution, keep stirring for a period of time, discard the oil layer and water layer, wash off the adsorbate on the surface of the particles, and obtain black magnetic chitosan microspheres with a more uniform particle size. Dissolve the cellulase with a weight rat...

Embodiment 3

[0015] Embodiment 3: first the FeSO of 1mol / L 4 ·7H 2 O and 1mol / L FeCl 3 ·6H 2 O was mixed at a molar ratio of 1:2, 5 mol / L NaOH was added, and vigorously stirred for 30 min to obtain an oily magnetic fluid suspension. 50mL 4% (w / v) chitosan solution, mixed with 3mL magnetic fluid, stirred evenly, added 50mL liquid paraffin, dripped emulsifier span80, stirred at high speed to make the system emulsion-like, added 4% glutaraldehyde by volume as Cross-linking agent, pour the fully reacted emulsion at 35-45°C into 10 times the volume of NaOH / ethanol (1:1) mixture, keep stirring for a period of time, discard the oil layer and water layer, and wash away the particles on the surface Adsorbate, to obtain black magnetic chitosan microspheres with uniform particle size. Dissolve cellulase with a weight ratio of 0.3 to the immobilized carrier in 0.05-0.4mol / L, HAc-NaAc buffer solution with a pH of 4.6-5.6, shake fully at room temperature, filter to remove the enzyme solution, and th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparing method of case oligosaccharide or Chitosan oligosaccharide with immobilized enzyme technology, which comprises the following steps: choosing reversal floating cross bridging method to produce magnetic chitose micro ball of multinuclear structure; using the micro ball as solidifying carrier; fixing cellulose on the micro ball as solidifying enzyme; using the solidifying enzyme to hydrolyze chitose; controlling the reacting time according to needs; getting the products. This method utilizes solidifying enzyme technology to increase stability of temperature and pH, which can be fir for food and medicine domain.

Description

technical field [0001] The invention relates to a method for preparing chitooligosaccharides or chitosan oligosaccharides by immobilized enzyme technology, which belongs to the field of chemical biology of renewable resources. Background technique [0002] Chitooligosaccharides and chitosan oligosaccharides are heteropolyoligosaccharides linked by N-acetyl-D-glucosamine or D-glucosamine through β-1,4-glycosidic bonds, which are produced by hydrolysis of chitin and chitosan A class of oligomers. In recent years, with the deepening of research, especially oligosaccharides with a molecular weight of less than 10,000 have shown their unique physiological activities and physicochemical properties. At present, the method of preparing chitooligosaccharides is mostly enzymatic hydrolysis, and chitosan and cellulose have similar chemical structures, so cellulase is often used as a non-specific chitosan hydrolase to degrade chitosan. However, since enzymes are composed of proteins, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12P19/04
Inventor 杜予民冯涛
Owner WUHAN UNIV