Preparation method and usage for novel lipopeptide type biosurfactant surfactin

A biological surface and lipopeptide technology, applied in the field of microorganisms, can solve the problems of unreachable commercial use and low yield, and achieve the effects of no environmental pollution and stable and simple fermentation process.

Inactive Publication Date: 2007-09-26
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, due to the low yield, it cannot be used commercially, so

Method used

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Embodiment Construction

[0026] 1. Screen and isolate Bacillus subtilis B.s with certain surface activity (the surface tension of Landy fermentation broth is 30.3mN / m) from the soil as the original strain.

[0027] 2. The ion implantation experiment of the original strain was carried out on the low-energy ion beam cell modification technology and device. The patent number of the device is 93103361.6. Use N + Pulse irradiation treatment, after 20keV, 1.3×10 14 N + / cm 2 ~5.2×10 15 N + / cm 2 , different doses of N + Injection, the process is: the sample is placed on a water-cooled sterile target platform, B.s is made into a bacterial suspension, and 0.1mL of the bacterial suspension is spread on a Φ90mm blank plate, and air-dried in an ultra-clean bench to make a bacterial film for use; with different doses of N + After the irradiation treatment, the bacterial film was eluted with 1mL sterile water, cultured in the blood plate solid medium for 24 hours, and then picked to remove the single colon...

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Abstract

The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g/L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.

Description

technical field [0001] The invention relates to the field of microbes, more specifically to a method for producing lipopeptide biosurfactin surfactin using a strain of Bacillus subtilis, and relates to its application range. Background technique [0002] Biosurfactants are metabolites produced by microorganisms under certain conditions with both hydrophilic and hydrophobic structures. In addition to having the same or similar physical and chemical properties as chemically synthesized surfactants, biosurfactants also have complex structures, strong specificity, low toxicity, biodegradability, and environmental friendliness, and can be produced by biological fermentation of cheap agricultural and sideline products. Biosurfactants also have pharmacological effects and physiological activities such as antibacterial, antiviral, and antitumor. Biosurfactants have been widely used in the fields of petroleum, food, cosmetics and pharmacy. [0003] In 1968, Arima et al. discovered ...

Claims

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Application Information

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IPC IPC(8): C12P21/02B01F17/30A61K8/64A01N25/30A23L1/03C11D3/18C12R1/125A23L29/00C09K23/30
Inventor 贡国鸿刘清梅袁成凌陈华樊永红周伟姚黎明王军郑之明吴李君吴跃进余增亮
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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