Method of accelerating human hypodermic fat stem cell differentiation and maturation and application thereof

A kind of stem cell differentiation and adipose stem cell technology, which can be used in skin trauma treatment and plastic surgery. The field of human subcutaneous adipose stem cells can solve the problems of cell survival and unfavorable culture

Inactive Publication Date: 2007-10-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

However, liposuction has a process of heating and liquefying subcutaneous fat, which is detrimental to the survival and cultivation of cells

Method used

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  • Method of accelerating human hypodermic fat stem cell differentiation and maturation and application thereof

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Embodiment 1

[0018] 1. Isolation, culture and expansion of adipose-derived stem cells

[0019] 1. The method of primary culture of adipose stem cells is as follows: Adult subcutaneous fat tissue comes from the gynecological surgery of Changhai Hospital. Use a sterile 50ml centrifuge tube to cut about 15ml of subcutaneous fat in the operating room, keep it at 37°C, and arrive within 30 minutes In the cell laboratory, use ophthalmic scissors on the ultra-clean workbench to cut adipose tissue into 0.3×0.3cm sized particles, and try to remove microvessels, connective tissue and electrocoagulation scars, and rinse the cut tissue with 0.1mM phosphate buffered saline (PBS) After 3 times, transfer it to a penicillin vial, digest with 0.1% collagenase I, and at the same time cut it repeatedly with ophthalmic scissors until it becomes a paste, transfer the paste tissue into a 50ml centrifuge tube, place it on a shaker at 37°C, 170rpm, continue digestion for 30min, and then centrifuge at 1000rpm for 1...

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Abstract

The invention relates to a stem cell and a gene engineering technique field. Embryo stem cell is provided with multidirectional differential energy, but the research and application has a plurality of ethic problems so that the research of adult stem cell with the same multidirectional differential energy is concerned, wherein the researchers attach importance to ADSC with abundant resources and a convenient extraction. FGF10 is an adult fiber cell growth factor which only expresses white fat tissue and has the important effect for differentiating and maturing fat tissue. The invention constructs a fat stem cell line by the external culture and a recombinant vector expressing adult fiber cell growth factor10 (FGF10), exogenesis expression FGF10 accelerates differentiation and mature of ADSC by verification of external and internal experiment. The method has application prospect in the cure of skin injury, shaping and beauty field.

Description

technical field [0001] The invention relates to the technical field of stem cells and genetic engineering, in particular to a method for using a recombinant human fibroblast growth factor 10 (FGF10) expression vector to act on human subcutaneous fat stem cells to differentiate and mature them, and its application in skin wound treatment and plastic surgery Applications in cosmetology. Background technique [0002] Human embryonic stem cells (hES) are a type of cell line obtained from the pre-implantation inner cell mass (ICM) with multilineage differentiation potential and can develop into various cells. Because of its pluripotency, it has become an important source of materials for various researches such as tissue engineering and organ transplantation. However, there are ethical issues in the research and application of embryonic stem cells. Therefore, the research on adult stem cells has attracted increasing attention in recent years. Among them, adipose tissue is rich i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08A61K35/12C12N5/10
Inventor 刘厚奇张迅轶
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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