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Wood hub erythrina arborescens water-alcohol extraction, medicine composition and method for producing these substances

A composition and drug technology, applied in the field of Erythrina by-products for preparing anti-anxiety drugs, can solve problems such as no pharmaceutical methods

Inactive Publication Date: 2007-10-24
圣保罗州立大学儒利奥清真学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although there are many methods, so far, the active ingredient of Erythrina japonica has not been isolated or developed into an anxiolytic agent through chemical synthesis, nor is there any method for preparing the agent

Method used

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  • Wood hub erythrina arborescens water-alcohol extraction, medicine composition and method for producing these substances
  • Wood hub erythrina arborescens water-alcohol extraction, medicine composition and method for producing these substances
  • Wood hub erythrina arborescens water-alcohol extraction, medicine composition and method for producing these substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1-Preparation and fractionation of extracts using plant materials

[0022] Collect flowers from adult trees in the winter in Rifaina County (SP). By using ethanol / water (EtOH / H 2 O) (7:3) dipping method The fresh plant material (6kg) is treated for 7 days for extraction. The extract was then filtered and concentrated by means of a rotary evaporator to obtain 292 g of dry hydroalcoholic extract. Then carry out the biological monitoring fractionation and separation of chemical composition. Then the acid-base extraction is carried out, with the purpose of optimizing the separation of Erythrina alkaloids. To achieve this purpose, the dry hydroalcoholic extract (120g) was dissolved in an aqueous solution of acetic acid (10%), and chloroform (CHCl 3 ) Perform liquid / liquid extraction. The chloroform phase was separated from the water phase and the solvent was evaporated to give fraction 1 (7.83 g). Then, use ammonium hydroxide (NH 4 OH) Alkalize the water phase, then use ...

Embodiment 2

[0023] Example 2-Chromatography, Instrument Operation and Spectrometry

[0024] Use Degree solvent for "analysis". For analysis, silica thin layer chromatography (CCD), CHCl 3 / Methanol (MeOH) (9:1) is used as the solvent system. The Dragendorf test of the alkaloid in F2 was positive, and the F2 was subjected to open-column chromatography (CCA) (diameter 5 cm, height 15 cm). For CCA (0,035-0,070mm, Φ6ηm), silica gel is used as a stationary phase, CHCl 3 / MeOH (10:0-8:2) was used as the mobile phase. For separation, 670 mg of F2 was used, and about 20 ml of 101 fractions were collected. The 101 fraction was carried out as mobile phase CHCl 3 / MeOH (7:1) analytical CCD and Dragendorf analysis showed that the 101 fraction was divided into fraction A (FA-136.2mg) (1-27), fraction B (FB-93.4mg) (28- 50), Fraction C (FC-148.3 mg) (51-69), Fraction D (FD-284.5 mg) (70-101). To separate and purify alkaloids, preparative thin-layer chromatography (CCDP) was used, fluoresceine was used as a...

Embodiment 3

[0030] Example 3-Pharmacological Evaluation

[0031] Swiss mice weighing 25-35 g obtained from the Central Animal Laboratory of Sao Paulo State University (UNESP / Araraquara) were used. The animals were divided into 10-12 animals / groups and raised in polypropylene cages filled with sawdust at the bottom, and adequate food and water were given. Keep the animal laboratory at a constant temperature of 22±1°C, control the light for a 12-hour cycle, from 7:00am to 7:00pm, and keep the humidity between 50-60%. The pharmacological evaluation was performed with the extract, standard drugs and carrier. Therefore, in addition to the use of F2 (3, 6, 10, 17 and 30 mg / kg) and the alkaloids Erythrina, erythravine and 11-OH-erythravine (3 and 10 mg / kg), freeze-dried hydroalcohol was also used The extract (50, 100, 200 and 400 mg / kg) was administered by oral ingestion using gavage. The standard drug used is diazepam (DZP) (by intraperitoneal administration, i.p) at a dose of 2 mg / kg. All solution...

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Abstract

To eliminate the contention with other packet communication service, when a logical connection necessary to provide a service supporting high-speed data communication referred to as an MBMS is configured on an Iu interface, in a mobile communications system. As a logical connection established between an SGSN 12 of CN10 and a radio controller 22 in a core network (CN) 10, a PS (packet-switching processing) functional connection 121 for an existing packet communication service and a connection 122 for a new MBMS service, offering high-speed data communication are provided separately and independently from each other. This eliminates the contention between different types of services of the PS service and the MBMS service, to thereby enable executing processings of these different types of services, without being conscious of mutual processings. In addition, release processing of the connection may be executed independently of each service, thereby eliminating the complexity of processings in the SGSN 12.

Description

Technical field [0001] The present invention relates to molecules that act on the cholinergic and / or serotonergic system. More specifically, the present invention relates to Erythrina by-products that can be used to prepare anti-anxiety drugs. A pharmaceutical composition containing the molecule and a method for preparing the pharmaceutical composition are also provided. Background technique [0002] Erythrina mulungu (Papilionaceae-Leguminoseae) is a woody plant with red flowers (10-14 meters high) that grows in the semi-deciduous broad Leaf forests and bushlands mainly in the western region of São Paulo State and the Minas Delta (LORENZI, 1992). The bark of this plant is used by the locals as a tranquilizer and tranquilizer. It is generally called mulungu, coral tree, coral wood hub and coral shrub (race names include capa-homem, suiná-suiná, tiricoiro, etc.) (LORENZI, 1992). Brazil produces eight species of Erythrina: E. mulungu, E. velurina, E. crita-galli, E. poeppigiana, E....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K31/4748
CPCA61K31/4748A61K36/48A61P23/00A61P25/08A61P25/20A61P25/22A61P29/00A61P43/00
Inventor B·范德兰·达·席尔瓦R·L·努涅斯·迪·索撒F·J·奥塔维沃·阿佩尔西多
Owner 圣保罗州立大学儒利奥清真学院