Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method
A fish collagen peptide, fish skin technology, applied in protein food processing, animal protein processing, food science and other directions
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Embodiment 1
[0034] Embodiment 2: 5000 kilograms of frozen fish bones are naturally thawed at room temperature, the fish bones are cleaned with clear water, drained, rinsed with warm water, and the cleaned fish skins are minced with a meat grinder. The minced fish aggregates are transferred to the emulsification tank, and 5-8 times the hot alkaline water ((pH8~9, 50~60°C)) is added for emulsification treatment. The emulsified material is put into the enzymatic hydrolysis tank. Heating makes Raise the temperature of the material to 50°C, and adjust the pH to 8.5 with the dissolved concentrated NaOH solution. Add enzyme A, and add enzyme A at an enzyme amount of 2000 to 10,000 units per gram of protein. During enzymolysis, keep the temperature at 50°C, and the pH Keep it at about 8.5, and continue enzymatic hydrolysis for 3 hours. Then cool the material in the enzymatic hydrolysis tank to about 40°C, adjust the pH to about 7, and add enzyme B at an enzyme amount of 5,000 to 10,000 units per gra...
Embodiment 2
[0035] According to the present invention, the molecular weight distribution of the fish collagen peptide is analyzed and determined by high performance gel filtration chromatography, and the molecular weight distribution diagram is shown in FIG. 1 . When the fish bone and skin raw materials, enzyme preparation and hydrolysis conditions are stable, the quality of the obtained fish collagen peptide is consistent, and the molecular weight distribution of the collagen peptide in the product is also relatively stable, which is shown in its characteristic molecular weight distribution diagram .
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