Method for preparing gene medication carrier by using chitosan modified liposome

A technology of drug delivery carrier and chitosan, applied in the field of gene drug delivery carrier preparation, can solve the problems of low transfection efficiency and low cytotoxicity, and achieve the effects of simple preparation process, good stability and reasonable design

Inactive Publication Date: 2007-10-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan has little cytotoxicity, but when chitosan is used alone as a gene carrier, its transfection efficiency is lower than that of liposome carrier

Method used

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  • Method for preparing gene medication carrier by using chitosan modified liposome
  • Method for preparing gene medication carrier by using chitosan modified liposome
  • Method for preparing gene medication carrier by using chitosan modified liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Take 10 to 200 mg of soybean phospholipids and 5 to 100 mg of cholesterol to 7.5 to 150 mL of chloroform, add 2.5 to 50 mL of distilled water, and prepare liposomes by reverse evaporation. Specifically, it is vortexed for 10-30 minutes to make it into milk, and the chloroform is removed by vacuum rotary evaporation on a rotary evaporator to obtain a liposome suspension. The liposome solution is ultrasonically treated with a 100~500W probe, working time is 1~3 seconds, gap time is 2~4 seconds, cycle time is 30~240 times, ultrasonic condition is ice bath, squeezed through membrane, pore size is 0.1~0.22 μm. Take chitosan with a molecular weight of 450kDa and dissolve it in glacial acetic acid with a concentration of 0.1% to 1%. The final concentration of the chitosan solution is 0.2% to 1.0%. The liposome solution is slowly injected into the chitosan solution for 1-10 minutes, and then stirred for 0.5-2 hours to obtain chitosan modified liposomes.

Embodiment 2

[0014] Example 2 Take 10 to 200 mg of lecithin and 5 to 100 mg of cholesterol to 7.5 to 150 mL of chloroform, add 2.5 to 50 mL of distilled water, and prepare liposomes by reverse evaporation. The liposome solution is ultrasonically treated with a 100~500W probe, working time is 1~3 seconds, gap time is 2~4 seconds, cycle time is 30~240 times, ultrasonic condition is ice bath, squeezed through membrane, pore size is 0.1~0.22 μm. Take chitosan with a molecular weight of 900kDa and dissolve it in glacial acetic acid with a concentration of 0.1% to 1%. The final concentration of the chitosan solution is 0.2% to 1.0%. The liposome solution is slowly injected into the chitosan solution for 1-10 minutes, and then stirred for 0.5-2 hours to obtain chitosan modified liposomes.

[0015] Prepared by the thin film dispersion method. Specifically: taking 10 to 200 mg of soybean phospholipids and 5 to 100 mg of cholesterol in 7.5 to 150 mL of chloroform and placing them in a pear-shaped bottle...

Embodiment 4

[0016] Example 4 Take 10 to 200 mg of lecithin and 5 to 100 mg of cholesterol to 7.5 to 150 mL of chloroform, prepare according to the above-mentioned film dispersion method, and add 2.5 to 50 mL of distilled water for ultrasonic hydration. The liposome solution is ultrasonically treated with a 100~500W probe, working time is 1~3 seconds, gap time is 2~4 seconds, cycle time is 30~240 times, ultrasonic condition is ice bath, squeezed through membrane, pore size is 0.1~0.22 μm. Take chitosan with a molecular weight of 900kDa and dissolve it in glacial acetic acid with a concentration of 0.1% to 1%. The final concentration of the chitosan solution is 0.2% to 1.0%. The liposome solution is slowly injected into the chitosan solution for 1-10 minutes, and then stirred for 0.5-2 hours to obtain chitosan modified liposomes.

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Abstract

The invention discloses a preparing method with chitose to modify liposome to prepare gene give medicine carrier, which comprises the following steps: building-up film material with phosphatide and cholesterol; adopting counter-current evaporating method or thin film dispersing method; getting liposome solution through probe hypersonic and squeezing film; allocating chitose solution with various molecular weight; hatching the chitose solution and liposome solution; getting the liposome. This invention can used as gene give medicine carrier, which possesses good internal application prospect.

Description

Technical field [0001] The invention belongs to a method for preparing gene delivery carriers, and relates to a method for preparing liposomes modified with chitosan. The modified liposomes can be used as gene delivery carriers. Background technique [0002] With the completion of the Human Genome Project, people have a deeper understanding of the genetic material basis of diseases. Gene therapy has become a promising treatment method, which is considered to treat hereditary congenital diseases and malignant tumors. , Infectious diseases and other most promising treatment options. [0003] Gene therapy refers to the use of gene transfer technology to introduce a target gene into target cells to express the gene to obtain a specific function, thereby achieving the purpose of treatment. The primary technical problem faced by gene therapy is the construction of gene vectors. Liposomes are enclosed particles with a double-layer membrane. As gene carriers, liposomes are mainly used to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K9/127A61K48/00
Inventor 高建青陈金亮何彩丽赵青青梁文权
Owner ZHEJIANG UNIV
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