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Method for synthesizing EGCG fatty acid ester catalyzed by immobilized enzyme

A technology for immobilizing lipase and fatty acid ester, applied in the field of enzyme catalysis, can solve problems such as the difficulty of EGCG phenolic hydroxyl reaction, and achieve the effects of being conducive to continuous production, increasing solubility, and simple separation and purification

Inactive Publication Date: 2007-12-12
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The invention adopts fatty acid vinyl ester with higher reactivity as an acyl donor, and solves the problem that lipase catalyzes the difficult reaction of EGCG phenolic hydroxyl

Method used

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  • Method for synthesizing EGCG fatty acid ester catalyzed by immobilized enzyme
  • Method for synthesizing EGCG fatty acid ester catalyzed by immobilized enzyme
  • Method for synthesizing EGCG fatty acid ester catalyzed by immobilized enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Take EGCG monomer 1.00g (2.18mmol), vinyl acetate 0.188g (2.18mmol), dissolve in 10ml ethyl acetate, add immobilized lipase 0.5g, under the condition of temperature 30 ℃, in the shaker (180r / min), reacted for 120 hours, and the EGCG conversion rate was 20%; the immobilized lipase was taken out, and the reaction solution was filtered, concentrated under reduced pressure, and desolvated to obtain 0.24 g of tan powdery EGCG acetate.

Embodiment 2

[0031]Take EGCG monomer 1.00g (2.18mmol), vinyl acetate 0.188g (2.18mmol), dissolve in 10ml ethyl acetate, add immobilized lipase 1g, under the condition of temperature 40 ℃, in the shaker (180r / min), reacted for about 120 hours, and the EGCG conversion rate was 25%; the immobilized lipase was taken out, and the reaction solution was filtered, concentrated under reduced pressure, and desolventized to obtain 0.30 g of tan powdery EGCG acetate.

Embodiment 3

[0033] Take EGCG monomer 1.00g (2.18mmol), vinyl acetate 0.188g (2.18mmol), dissolve in 10ml tetrahydrofuran, add immobilized lipase 2.0g, under the condition of temperature 40 ℃, in the shaker (180r / min ), reacted for 120 hours, and the EGCG conversion rate was 30%; the immobilized lipase was taken out, and the reaction solution was filtered, concentrated under reduced pressure, and desolvated to obtain 0.35 g of tan powdery EGCG acetate.

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Abstract

The invention relates to a method for preparing EGCG fatty acid ester by using immobilized lipase, belonging to enzyme catalysis field. It comprises following steps: taking catechin EGCG monomer and aliphatic acid or fatty acid vinyl ester containing 2- 18 carbon atoms as raw material, mixing with sifted organic solvent and immobilized lipase according to molar ratio of 1: 1- 10, the enzyme consumption amount is 0.5- 3 times of that of EGCG, reacting for 36- 200 hours at 30 -50 Deg. C, taking out catalyst, filtering raction liquid, decompressing and condensing, removing solvent and drying and getting EGCG fat lipase. The invention is characterized by good selectivity, high conversion rate, non toxic reaction solvent, definite EGCG lipase component, high content of monosubstituted product, good anti- oxidizing performance and stable structure.

Description

technical field [0001] The invention relates to a method for synthesizing EGCG fatty acid ester catalyzed by immobilized lipase, in particular to a method for synthesizing EGCG fatty acid ester by catalyzing reaction with immobilized microbial lipase in a non-aqueous phase system, belonging to the field of enzyme catalysis. Background technique [0002] Tea polyphenols in green tea have been widely used in food, cosmetics and other industries because of their strong free radical scavenging ability and antioxidant activity. They are a rare natural antioxidant. At the same time, tea polyphenols have important physiological and pharmacological functions such as anti-cancer, anti-aging, anti-radiation, lowering blood sugar and blood lipids. The development and utilization of tea polyphenols and their derivatives have attracted great attention at home and abroad, and many documents and invention patents have disclosed their applications as antioxidants, anticancer and antiviral d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/72
Inventor 王芳马习东谭天伟邓利聂开立
Owner BEIJING UNIV OF CHEM TECH
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