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Homotype semicystinol diagnostic kit and homotype semicystinol concentration investigating method

A technology of homocysteine ​​and diagnostic kits, which is applied in the field of medical inspection and measurement, and can solve problems such as complicated operation, long test time, and large variation of test data.

Inactive Publication Date: 2007-12-19
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The homocysteine ​​diagnostic reagent of this embodiment is a single reagent, including:

[0043] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 50mmol / L

[0045] 5-Methyltetrahydrofolate 10mmol / L

[0046]Methionine synthase 6000U / L

[0047] Amino acid oxidase 10000U / L

[0048] 10-(Carboxymethyl-nitroacetyl)-3,7-bis(dimethylamino)phenothiazine

[0049] 2mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 660nm, test sub-wavelength 800nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, reaction direction is Positive reaction (rising reaction), the detection method is the endpoint method, the delay time is about 0 minu...

Embodiment 2

[0054] The homocysteine ​​diagnostic reagent of this embodiment is a dual reagent, including:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Amino acid oxidase 15000U / L

[0059] 10-(Carboxymethyl-nitroacetyl)-3,7-bis(dimethylamino)phenothiazine

[0060] 3mmol / L

[0061] Reagent 2

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] 5-Methyltetrahydrofolate 16mmol / L

[0065] Methionine synthase 10000U / L

[0066] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0067] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 660nm, test sub-wavelength 800nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 20, reaction direction is Positive ...

Embodiment 3

[0069] Embodiment three homocysteine ​​diagnostic reagents are three reagents, including:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] 10-(Carboxymethyl-nitroacetyl)-3,7-bis(dimethylamino)phenothiazine

[0074] 2mmol / L

[0075] Reagent 2

[0076] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0077] Stabilizer 50mmol / L

[0078] Amino acid oxidase 14000U / L

[0079] Reagent 3

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 50mmol / L

[0082] 5-Methyltetrahydrofolate 12mmol / L

[0083] Methionine synthase 8000U / L

[0084] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0085] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 660nm, t...

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Abstract

A diafnosis kit of homocysteine consists of compositions as buffer solution; 5-methyltetrahydrofolate; methyltetrahydrodioperin-homocysteine- methyltransferase; amino acid oxidase; 10 ( carboxymethyl-N2 -acetyl) -3, 7-dual ( dimethylamino) phenothiazine (DA-67) and stabilizer. Its method for determining concentration of homosteine is also disclosed.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnostic kit, and at the same time, the invention also relates to a method for measuring the concentration of homocysteine, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic methods superseded...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78G01N33/50G01N33/52G01N33/68C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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