Biological molecule high flux quantitative detection method

Abstract

The invention disclose the method of a high pass and fixed quantity detect of bimolecular, it use the method of coding molecule to achieve high pass and fixed quantity detect, it can markedly improve the efficiency of correlation application within the field of the research biology, the prove of clinic and eatable sanitation, the detect of environment and the prove of medical jurisprudence etc.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-throughput quantitative detection method for biomolecules. Background technique [0002] In biological research and clinical diagnosis, it is often necessary to know how many biomolecules and their contents are contained in research or clinical specimens. Before the advent of high throughput detection methods (high throughput detection methods), it was necessary to know how many biomolecules existed in a sample, and how many times the sample needed to be tested. The number of specimens is often limited, it is difficult to perform too many tests on them, and it is extremely difficult to obtain a large amount of test information. Therefore, high-throughput quantitative detection technology can not only greatly improve the detection efficiency of biological research and experimental diagnosis, but also greatly improve the information abundance that can be obtained fr...

AI Technical Summary

Problems solved by technology

[0007] Although xMAP technology has some significant advantages over Biochips technology, such as almost no steric hindrance effect, fast hybridization reaction and high efficiency; the repeatability is greatly improved, xMAP can greatly improve the detection of multiple microspheres of the same color by repeated detection However, xMAP technology also has some significant deficiencies: (1) The detection throughput is limited. Due to the fluorescent coding, a maximum of 100 polypropylene microspheres of different colors can be obtained. xMAP’s The detection throughput cannot exceed 100 kinds of TM / test; (2) Application development is difficult and costly. For each type of TM detected by xMAP, two different BREs need to be developed, which increases the difficulty, cycle and time of application development. (3) There is no accurate basis for quantitative analysis. In xMAP technology, the fluorescence intensity of each microsphere-TM-fluorescent reporter complex can be measured, and multiple same-color The proportion of microspheres bound with fluorescent reporter molecules in the microspheres, this information can be used to analyze the content of the corresponding TM, but because the amount of cross-linked BRE in each microsphere cannot be exactly the same, according to Microsphere-TM- There will be a large error in the quantification of the fluorescence intensity of the fluorescent reporter molecular complex. Due to the randomness of sampling, the microspheres collected for each analysis are different, so the proportion of luminescent microspheres for each test cannot be exactly the same. Therefore, xMAP can only conduct quantitative analysis in a statistical sense based on the above two indicators, but cannot give a quantitative formula with direct relationship

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