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Method for preparing low-lactose whey powder containing galactooligosaccharide

A technology of galacto-oligosaccharides and whey powder, which is applied in the direction of whey, dairy products, fungi, etc., can solve the problems of time-consuming, labor-intensive, high cost, cumbersome purification process, etc.

Active Publication Date: 2010-05-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of these two methods are that the purification process of the enzyme is cumbersome, time-consuming and labor-intensive, the yield of the enzyme protein is low, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0010] A preparation method of low-lactose whey powder containing galacto-oligosaccharides, the steps are as follows:

[0011] (1) Inoculate one of the lactic acid bacteria strains producing transglycosyl β-galactosidase in the MRS medium for activation, and inoculate the activating bacteria in the enzyme-producing fermentation broth with an inoculation amount of 1-10% (v / v) Medium, 30 ~ 45 ℃ static culture for 16 ~ 24 hours;

[0012] The above-mentioned MRS medium, its components are: glucose 10g / L, tryptone 10g / L, beef extract 10g / L, yeast extract 5g / L, diammonium citrate 2g / L, dipotassium hydrogen phosphate 2g / L, MgSO 4 ·7H 2 O 0.58g / L, MnSO 4 ·H 2 0.25g / L, Tween 80 is 1mL / L;

[0013] The above enzyme-producing fermentation medium depends on the selection of lactic acid bacteria strains, and its components at least contain: 5-20% volume ratio of 10 Baumé wort as the base medium, 1-10g / L peptone, 1-10g / L Beef extract and 1-10g / L yeast powder;

[0014] (2) Centrifuge t...

Embodiment 1

[0026] The lactic acid bacteria strain producing transglycosyl β-galactosidase is Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842.

[0027] After inoculating Lactobacillus delbrueckii subsp.bulgaricus ATCC 11842 into MRS medium for activation, inoculate 2% (v / v) inoculum in the enzyme-producing fermentation medium, and stand at 37°C Cultivate for 18 hours, after the end of the cultivation, centrifuge at 10,000 rpm for 10 minutes, and collect the lactic acid bacteria cells.

[0028] The formula of the above enzyme-producing fermentation medium is 10% 10 Baumé wort as the base medium, supplemented with 5g / L peptone, 3g / L beef extract and 5g / L yeast powder.

[0029] Take 1g of the above-prepared lactic acid bacteria cells and suspend them in 3mL of 50mmol / L potassium phosphate buffer solution with pH 7.0 to make a lactic acid bacteria cell suspension. In the lactic acid bacteria cell suspension, the wall is broken at 37°C for 1 hour, and the obtained suspension is the cru...

Embodiment 2

[0038] As described in Example 1, the difference is that the transglycosyl β-galactosidase-producing lactic acid bacteria strain used is Lactobacillus reuteri (Lactobacillus reuteri) ATCC 55148.

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Abstract

The invention relates to a simple and rapid preparation method for the low lactose whey powder with oligomerization galactose, which comprises a bacterial strain choice, culture and enzyme production,a collection of lactic acid bacteria cell, a glycosylation B galactose enzyme solution is prepared by utilizing the bacteriolysis enzyme wall-broken bacteria cell, and the whey powder is processed byutilizing the enzyme solution. The invention has the advantages that: the lactose content of the whey powder can be lowered greatly, and the high-content and oligomerization galactose can be made; the process is simple and reasonable, thereby the complex purification process of enzyme protein can be avoided with time saving and labor saving; the production costs are lowered; so the utility modelhas vast industrialized application prospect.

Description

technical field [0001] The invention relates to a preparation method of low-lactose whey powder, in particular to a preparation method of low-lactose whey powder containing galacto-oligosaccharide. technical background [0002] 60-80% of whey powder is lactose, which is mainly used in the production of dairy products in the food industry. The results of clinical tolerance tests show that 50-100% of people who ingest 50 grams of lactose will experience symptoms of lactose indigestion, that is, lactose intolerance. Lactose intolerance refers to the lack of lactase (also known as β-galactosidase) in the body, resulting in lactose cannot be digested and absorbed in the small intestine and directly transported to the large intestine, causing fermentation and hydrolysis under the action of bacteria, resulting in intestinal A large amount of gas such as water and carbon dioxide in the intestinal tract accumulates, and stimulates the intestinal mucosa to cause a series of symptoms ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C21/02C12N1/14
Inventor 肖敏李正义卢丽丽
Owner SHANDONG UNIV