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Method for preparing sodium L-aspartate by bio-enzyme

A technology of sodium aspartate and aspartase, which is applied in the field of preparing sodium L-aspartate, can solve the problems of high production cost, environmental pollution, and high price of L-aspartic acid, and achieve production cost Low, no environmental pollution effect

Inactive Publication Date: 2008-02-27
ANHUI HUAHENG BIOTECH
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Problems solved by technology

This method has following shortcoming: the one, and production cost is high, and the price of finished product L-aspartic acid is higher, has adverse effect to popularization of product; The production of raw material L-aspartic acid sodium requires a large amount of liquid ammonia and sulfuric acid to produce a large amount of wastewater with high ammonia nitrogen content. Every ton of L-aspartic acid produced will produce more than ten tons of wastewater, of which sulfuric acid Ammonia content up to 5% or more

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  • Method for preparing sodium L-aspartate by bio-enzyme
  • Method for preparing sodium L-aspartate by bio-enzyme
  • Method for preparing sodium L-aspartate by bio-enzyme

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Embodiment Construction

[0010] Known by reaction equation of the present invention, the molar concentration ratio of fumaric acid, sodium hydroxide and ammonia should be 1: 1: 1, but in actual production process, in order to improve the conversion rate of fumaric acid, often increase sodium hydroxide and The amount of ammonia, but the amount of the two increases too much and it will cause waste and difficulty in separating L-sodium aspartate, so the molar concentration ratio of fumaric acid, sodium hydroxide and ammonia is controlled at 1:1:1~ 1:2:2, as a preferred version of the present invention, the molar concentration ratio of fumaric acid, sodium hydroxide and ammonia in the substrate solution is 9:10:10, such a ratio can make the conversion of fumaric acid The efficiency is the largest, and the residual amount of sodium hydroxide and ammonia is small, which will not cause waste, and is convenient to separate and obtain L-sodium aspartate from the reaction solution.

[0011] The pH value of the ...

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Abstract

The present invention relates to a method for preparing sodium aspartate by utilizing biological enzyme catalysis one-step process. Said method includes the following steps: mixing fumaric acid, sodium hydroxide and ammonia according to the mole ratio of 1:1:1-1:2:2 to obtain substrate solution whose pH value is 8.0-9.0, then adding fermentation liquor containing aspartase into said substrate solution, the volume ratio of the described substrate solution and fermentation liquor is 16:1-16:3, making reaction for 12-20hr under the condition of normal pressure and 37deg.C-40deg.C, then making separation so as to obtain the invented sodium aspartate.

Description

technical field [0001] The invention relates to a method for preparing sodium L-aspartate in one step through biological enzyme catalysis. Background technique [0002] Sodium L-aspartate is an amino acid umami agent, the second most used amino acid umami agent after sodium glutamate. It is a replacement product of monosodium glutamate. It can be used alone or combined with nucleic acid And other series of seasonings are used together, and it has the special effect of relieving human fatigue, and the demand is large and the market is vast. [0003] The methods for producing sodium L-aspartate both at home and abroad at present all adopt the chemical synthesis method, and the raw materials used are L-aspartic acid and sodium hydroxide. This method has following shortcoming: the one, and production cost is high, and the price of finished product L-aspartic acid is higher, has adverse effect to popularization of product; The production of raw material L-aspartic acid sodium r...

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Application Information

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IPC IPC(8): C12P13/20A23L1/227A23L27/21
Inventor 黄建坡郭恒华蒋光玉唐思青郑向辉
Owner ANHUI HUAHENG BIOTECH
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