Method for detecting volatile and semi-volatile organic acid in tobacco leaf or cut tobacco

Abstract

The detection method of the volatile and semi-volatile organic acid in tobacco leaf or thread is provided in invention. The character is in that: it uses the accelerating solvent extraction device (ASE200, Dionex, USA). In the condition of setting pressure, temperature and time, the volatile and semi-volatile organic acid of C1-C18, which is derived from the N,O-BSTFA and detected by the GC-MS. The extracting solvent is little and the time is short. The method is simple and has good repeatability, high detection sensitivity; also it can detect the volatile and semi-volatile organic acid at the same time. So it is proper for detecting big batch of samples.

Description

technical field [0001] The invention belongs to a method for measuring chemical components, in particular to a method for measuring volatile and semi-volatile organic acids in tobacco leaves or shredded tobacco. In this method, the Dionne 200 accelerated solvent extraction device is used to extract C1-C18 volatile organic acids and higher fatty acids at a time under a certain temperature and pressure. ) for analytical determination. By adopting the analysis method, the volatile and semi-volatile organic acids can be measured simultaneously, which changes the previous situation that different analysis methods are used for the determination of the volatile and semi-volatile organic acids. technical background [0002] The organic acids in tobacco we usually refer to mainly refer to organic acids other than amino acids. Generally, organic acids are divided into volatile, semi-volatile and non-volatile organic acids according to the strength of volatility. Usually, lower fatty...

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Problems solved by technology

Wherein, solid-phase microextraction, headspace separation are suitable for qualitative analysis of volatile organic acids, and the reproducibility is not good for quantitative analysis (Chen Zhangyu, Tobacco Science and Technology, 2000 (8)); For the higher formic acid in tobacco , acetic acid, propionic acid, butyric acid and other low-molecular-weight fatty acids, because of their strong water solubility and strong volatility, it is difficult to accurately quantitatively analyze them and higher fatty acids at the same time by using traditional steam distillation and organic solvent extraction methods. The liquid chromatography and capillary electrophoresis method are also difficult to do this; and other analysis methods use many organic solvents, the analysis time is long, the process is complicated, and the loss of volatile components is large (Li Yun, low-level fatty acids in domestic flue-cured tobacco and oriental tobacco Research