Legionella pneumophila biochemical reaction identification method

Abstract

The invention relates to an appraising method for legionella pneumophilia biochemical reaction. The invention comprises four steps of tests: hippurate hydrolyzing test, nitrate deacidizing test, urease test and gelatin liquefaction test. The key technique is that an L-cysteine is respectively added into the culture medium of the hippurate hydrolyzing test, the nitrate deacidizing test, the urease test and the gelatin liquefaction test of the biochemical reaction. The invention can be applied to appraising legionella pneumophilia of the environmental water, air-conditioning cooling water, condensate water, phlegm, gas pipe suction liquid, lung tissue, etc. The invention can be applied to disease prevention and control system, Entry-Exit Quarantine Bureau, technical supervision department, environmental protection, hospital and other industries. The invention has the advantages of simple technique, easy operation, fast testing speed and high efficiency. The invention provides a novel scientific method for quickly detecting and appraising legionella pneumophilia.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a biochemical reaction of Legionella pneumophila, in particular to an identification method for the biochemical reaction of Legionella pneumophila. Background technique [0002] The biochemical reaction of microorganisms refers to the use of chemical reactions to determine the metabolites of microorganisms. It is often used to identify some microorganisms that are not easy to distinguish in form and other aspects. It is one of the important basis for the classification and identification of microorganisms. Different types of bacteria, due to the different metabolic enzymes in their cells, have great differences in the absorption and utilization of nutrients, decomposition, excretion and production of synthetic products. The biochemical test of bacteria is to detect whether a certain bacteria can use A certain (some) substance is extremely metabolized and synthesized to a certai...

AI Technical Summary

Problems solved by technology

At present, many identification methods used in China are based on the characteristics of the growth of this bacteria on different media, according to the colony morphology, Gram staining, the characteristics of growth on different media, serological identification, and PCR methods. There are not many identifications of biochemical reactions. The reason is that the existing biochemical reactions are difficult to confirm. At present, there is no commercial product specifically for the identification of specific biochemical reactions of Legionella pneumophila in China.