Tobacco gamma-ray and mutagenesis idioplasm innovation and breed selecting and cultivating technique
A gamma ray and tobacco technology, applied in the fields of seed and rhizome treatment, plant genetic improvement, botany equipment and methods, etc., can solve the problems of narrow dose range and limited guiding significance, and achieve clear and strong radiation absorbed dose and dose rate Practicality, the effect of improving breeding efficiency
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[0016] Embodiment The present invention is carried out according to the following steps:
[0017] The first step is the selection of mutagenic materials: choose K326, which has good appearance and internal chemical quality, and resistance to various diseases, but has fewer leaves, poor upper leaf opening, and low yield when planted in Guizhou Province. The air-dried seeds of the variety were used as mutagenic materials. The purpose of mutagenesis is to select and breed K326 mutation material with more leaves and better opening of upper tobacco leaves.
[0018] The second step, seed treatment: about 10,000 K326 air-dried seeds were equilibrated in a desiccator filled with 50% glycerol monowater solution for 7 days, and then irradiated at a dose rate of 0.8Gy / min and an absorbed dose of 350Gy to obtain M 1 generation seeds.
[0019] In the third step, M 1 Planting and management of generations: M 1 The sowing, seedling raising, transplanting and field management of the new g...
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