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Tobacco gamma-ray and mutagenesis idioplasm innovation and breed selecting and cultivating technique

A gamma ray and tobacco technology, applied in the fields of seed and rhizome treatment, plant genetic improvement, botany equipment and methods, etc., can solve the problems of narrow dose range and limited guiding significance, and achieve clear and strong radiation absorbed dose and dose rate Practicality, the effect of improving breeding efficiency

Inactive Publication Date: 2008-05-07
GUIZHOU TOBACCO SCI INST
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AI Technical Summary

Problems solved by technology

Existing data report: the appropriate dose of tobacco γ-ray mutagenic seeds is 50-1,000Gy (Tong Daoru. Tobacco Breeding. Beijing: China Agricultural Press, 1997: 269), because this dose range is too large, it is not suitable for practical application. limited guidance
But so far, a relatively narrow dose range and a treatment dose and corresponding technical system that can induce mutations efficiently have not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Embodiment The present invention is carried out according to the following steps:

[0017] The first step is the selection of mutagenic materials: choose K326, which has good appearance and internal chemical quality, and resistance to various diseases, but has fewer leaves, poor upper leaf opening, and low yield when planted in Guizhou Province. The air-dried seeds of the variety were used as mutagenic materials. The purpose of mutagenesis is to select and breed K326 mutation material with more leaves and better opening of upper tobacco leaves.

[0018] The second step, seed treatment: about 10,000 K326 air-dried seeds were equilibrated in a desiccator filled with 50% glycerol monowater solution for 7 days, and then irradiated at a dose rate of 0.8Gy / min and an absorbed dose of 350Gy to obtain M 1 generation seeds.

[0019] In the third step, M 1 Planting and management of generations: M 1 The sowing, seedling raising, transplanting and field management of the new g...

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Abstract

The invention discloses a tobacco Gamma ray seed quality induction mutation innovation and variety selection technology. The technology comprises: firstly, material selection, air dried seeds of the tobacco cultivating variety, which has better comprehensive traits and strong adaptability, are selected; secondly, seed treatment, the dosage of Gamma ray is 350Gy, the dosage rate is 0.8 to 1Gy, and the treated seed number is more than 10 000; thirdly, M1 planting and management, the planting density is enlarged, and the appropriate planting group is 5000 to 8000 plants; the seeds are harvested according to the method of one tree with less grains, and the seeds are mixed according to the seed type; fourthly, M2 planting and management, the appropriate planting group is 8000 to 10000 plants; fifthly, M3 and the sequent treatment, the single plant selected from the M2 generation is planted into the M3 generation plant line, each plant line has 150 to 200 plants. The technology has the following advantages: firstly, the radiation absorption dosage and the dosage rate are clear, thereby being advantageous for the actual operation and the enhancement of the selection rate; secondly, the selection technology integrates the simplicity and the practicability into a whole, and the selection effect and the efficiency are enhanced to the maximum degree under the minimum group scope and investment.

Description

technical field [0001] The invention relates to a seed treatment method, in particular to tobacco germplasm innovation and variety breeding technology. technical background [0002] The use of physical factors such as electromagnetic radiation and ion radiation to induce mutations for crop germplasm innovation and variety breeding is an important part of the peaceful use of atomic energy. Due to the high mutation frequency and wide mutation spectrum of this method, it can break the chain of traits and accelerate the mutation of traits. It has been widely used in germplasm innovation and new variety breeding of crops due to its stability and other advantages. In terms of treating tobacco seeds with γ-rays, there are technical solutions that have applied for patents, such as Chinese patent application No. 97119883.7 "Gamma-ray irradiation treatment process for flue-cured tobacco seeds", No. breeding methods", etc. Appropriate mutagen dosage and breeding technique are the key...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H1/04A01C1/00
Inventor 任学良李明海王轶史跃伟
Owner GUIZHOU TOBACCO SCI INST
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