Laser double-mode micro-volume sample analyzing method and its device
A sample analysis and micro-volume technology, applied in the field of analytical chemical instruments, can solve the problem of difficulty in obtaining macromolecules and small molecules at the same time, and achieve the effects of low daily maintenance costs, accurate results, and simple device operation.
Inactive Publication Date: 2008-06-11
WUHAN UNIV
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Problems solved by technology
However, due to the limitations of commercial detectors, it is difficult to simultaneously obtain information on large and small molecules, as well as substances with and without fluorescent and light-absorbing properties
Method used
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[0023]The optical structure of the detection device used in the laser dual-mode microvolume sample analysis method based on laser thermal lens and laser retroreflection interference technology in the present invention is shown in Figure 1, at least including an optical detection modulation part, a capillary electrophoresis part and a signal acquisition and processing part, wherein The semiconductor laser 1, optical chopper 2, polarizer 3, beam splitter 4, beam splitter 6, focusing lens 9, micro detection cell 10, high-pass filter 11 and photoelectric detection device 12 in the optical detection modulation part are arranged in sequence , and the centers are all located on the same horizontal line. The beam splitter 4 is provided with a thermal lens detection laser 5 in the vertical direction. The capillary of protective layer; Lock-in amplifier 19 is provided between computer 21 and photodetection device 8 in the signal acquisition processing part, is provided with lock-in ampli...
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Abstract
The invention discloses a laser dual-mode microvolume sample analyzing method based on laser thermal lens and laser retroreflection interference technique and the used device. Under the wavelength of the used laser, test the signal of the substance with light adsorbing characteristic by the laser thermal lens testing mode to each component through liquid phase separation, and test the signal of the substance without fluorescent and light adsorbing characteristic by the laser retroreflection interference test mode. The invention doesn't need to conduct any sample deriving and collecting processing procedures. Because the required checking sample poor is micro-checking pool, the needed sample volume is very small and can reach nano flow at lowest. The used device in the invention is simple to operate, which has low daily maintaining fee, quick testing speed and accurate result, and can be widely used in the characterization and quantification testing to the need checking system including various different chemical substances.
Description
technical field [0001] The invention relates to a micro-volume sample analysis method and a device used, in particular to an analysis method and a device for simultaneously detecting a micro-volume sample by using laser thermal lens and laser retroreflection interference technology, which belong to the field of optics and also belong to the field of analytical chemical instruments. Background technique [0002] Liquid phase separation and analysis instruments are the most rapidly developed type of analytical instruments in recent years, including high performance liquid chromatography (HPLC), capillary electrophoresis (CE), ion chromatography, supercritical fluid chromatography (SFC) and microfluidic chips and other instruments , This type of instrument has the characteristics of high efficiency, rapidity, low sample consumption and low reagent consumption. It can be used for the separation and analysis of inorganic, organic and biological macromolecules, and has been widely...
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Patent Timeline
Login to View More IPC IPC(8): G01N21/00G01N21/17G01N21/45G01N30/74G01N30/78
CPCG01N21/171G01N21/45G01N21/6402G01N2021/1712
Inventor 胡继明熊博邓延倬
Owner WUHAN UNIV